Processos de inclusão: uma análise do percurso formativo de alunos surdos do IFB Campus Gama

Dissertação apresentada para obtenção do grau de Mestre em Educação Social e Intervenção Comunitária

SnaPshot based genotyping of the RYR1 mutation in Portuguese breeds of pigs

The porcine stress syndrome or malignant hyperthermia is an inherited autosomic recessive disease, which results in neuromuscular disorders leading to death in homozygous individuals and is associated with deterioration of meat quality. The defect in susceptible animals results from modifications in the calcium release channel or Ryanodine Receptor (RYR1), with a mutation leading to a C to T transition in nucleotide 1843 of the gene. The objective of this work was to develop a method based on analysis of SNPs to detect the mutation described in the RYR1 locus in pigs, and study polymorphisms of the gene in four exotic (Large White, Landrace, Duroc and Pietrain) and three native (Bísaro, Alentejano and Malhado de Alcobaça) breeds of pigs in Portugal. The method was successful in identifying the mutation by analysis of SNPs, and results indicate a high incidence of the mutant allele in Pietrain (0.75) and, to a lesser degree, in Malhado de Alcobaça (0.34) and Landrace (0.28); frequencies in Alentejano, Bísaro and Large White ranged between 0.04 and 0.09. These results suggest the need to establish breeding programs aimed at eliminating the susceptibility allele from those populations.

Factores que influenciam o peso e crescimento de cabritos da raça Murciana-granadina, em aleitamento artificial

A exploração caprina de leite tem evoluído no sentido de alguma intensificação, com recurso a raças de elevado potencial produtivo, de que é exemplo a raça Murciana- Granadina. O leite constitui a principal fonte de receita destas explorações. Complementarmente, vendem animais para carne e, as de melhor nível genético, animais para reprodutores. Analisaram-se os pesos de 241 cabritos da raça Murciana-Granadina, numa exploração comercial, com o objectivo de quantificar os pesos e crescimento de cabritos, e identificar os factores que os influenciam. Os cabritos foram aleitados artificialmente, em regime ad libitum, com leite de substituição comercial, dispondo ainda de concentrado comercial, feno de luzerna e palha. Os cabritos foram pesados ao nascimento e, posteriormente, semanalmente, até aos 60 dias de idade. Calcularam-se os respetivos pesos ajustados, bem como os ganhos médios diários, a diferentes idades padrão. Procedeu-se a uma análise de variância com um modelo linear que incluiu os efeitos da época de parto, tipo de parto, sexo e idade da cabra. Foram registados pesos superiores nos partos simples e duplos, relativamente aos triplos, e nos machos, relativamente às fêmeas. Os ganhos médios diários, a partir do mês de idade, registaram valores inferiores na época inverno-primavera, comparativamente com a época primavera-verão. Dairy goat farming has evolved towards intensification, with increased use of high milk-yielding breeds, including the Murciano-Granadina breed. Milk is the main source of farm income. Secondary income sources are the sale of animals for meat and, in genetically superior herds, the sale of breeding animals. The weights of 241 commercial farms artificially reared Murciano-Granadina kids were analyzed with the objective of quantifying weight and growth and identifying variation factors. Kids were artificially reared to weaning, on ad libitum commercial milk replacer, commercial concentrate, lucerne hay and straw. Kids were weighed at birth and at weekly intervals until 60 days of age. Age adjusted weights and growth-rates were calculated. A variance analysis was performed with a model including the effects of season of birth, number of kids per kidding, sex and age of dam. Single and twin-born kids had higher weights than triplets, and males had higher weights than females. Average daily gain after one month of age was lower for kids born in winter-spring than for those born in spring-summer

Genetic structure, relationships and admixture with wild relatives in native pig breeds from Iberia and its islands

Background: Native pig breeds in the Iberian Peninsula are broadly classified as belonging to either the Celtic or the Mediterranean breed groups, but there are other local populations that do not fit into any of these groups. Most of the native pig breeds in Iberia are in danger of extinction, and the assessment of their genetic diversity and population structure, relationships and possible admixture between breeds, and the appraisal of conservation alternatives are crucial to adopt appropriate management strategies. Methods: A panel of 24 microsatellite markers was used to genotype 844 animals representing the 17 most important native swine breeds and wild populations existing in Portugal and Spain and various statistical tools were applied to analyze the results. Results: Genetic diversity was high in the breeds studied, with an overall mean of 13.6 alleles per locus and an average expected heterozygosity of 0.80. Signs of genetic bottlenecks were observed in breeds with a small census size, and population substructure was present in some of the breeds with larger census sizes. Variability among breeds accounted for about 20% of the total genetic diversity, and was explained mostly by differences among the Celtic, Mediterranean and Basque breed groups, rather than by differences between domestic and wild pigs. Breeds clustered closely according to group, and proximity was detected between wild pigs and the Mediterranean cluster of breeds. Most breeds had their own structure and identity, with very little evidence of admixture, except for the Retinto and Entrepelado varieties of the Mediterranean group, which are very similar. Genetic influence of the identified breed clusters extends beyond the specific geographical areas across borders throughout the Iberian Peninsula, with a very sharp transition from one breed group to another. Analysis of conservation priorities confirms that the ranking of a breed for conservation depends on the emphasis placed on its contribution to the betweenand within-breed components of genetic diversity. Conclusions: Native pig breeds in Iberia reveal high levels of genetic diversity, a solid breed structure and a clear organization in well-defined clusters.