Detection of 2-substituted cyclobutanones as irradiation products of lipid-containing foods: Synthesis and applications of cis- and trans-2-(tetradec-5'-enyl)cyclobutanones and 11-(2'-oxocyclobutyl)-undecanoic acid

cis- (3(cis)) and trans-2-(tetradec-5'-enyl)cyclobutanone (3(trans)) have been chemically synthesised and used in the unambiguous identification of the cis isomer 3(cis) in irradiated meat (example chicken) and fruit (example papaya). 11-(2'-Oxocyclobutyl)undecanoic acid 5 has been chemically synthesised, conjugated to bovine thyroglobulin and used to generate polyclonal antibodies in rabbits, which have been used in the development of an enzyme-linked immunosorbent assay for the detection of 2-substituted cyclobutanones in irradiated chicken meat.