Calibrated Polarisation Tilt Angle Recovery for Wireless Communications

In this paper, we show how the polarisation state of a linearly polarised antenna can be recovered through the use of a three-term error correction model. The approach adopted is shown to be robust in situations where some multipath exists and where the sampling channels are imperfect with regard to both their amplitude and phase tracking. In particular, it has been shown that error of the measured polarisation tilt angle can be improved from 33% to 3% and below by applying the proposed calibration method. It is described how one can use a rotating dipole antenna as both the calibration standard and as the polarisation encoder, thus simplifying the physical arrangement of the transmitter. Experimental results are provided in order to show the utility of the approach, which could have a variety of applications including bandwidth conservative polarisation sub-modulation in advanced wireless communications systems.

A Loop-mediated Isothermal Amplification Method for Rapid Direct Detection and Differentiation of Non-pathogenic and Verocytotoxigenic Escherichia coli in Beef and Bovine Faeces

The aim of this study was to develop a multiplex loop-mediated isothermal amplification (LAMP) method capable of detecting Escherichia coli generally and verocytotoxigenic E. coli (VTEC) specifically in beef and bovine faeces. The LAMP assay developed was highly specific (100%) and able to distinguish between E. coli and VTEC based on the amplification of the phoA, and stx1 and/or stx2 genes, respectively. In the absence of an enrichment step, the limit of detection 50% (LOD50) of the LAMP assay was determined to be 2.83, 3.17 and 2.83-3.17 log CFU/g for E. coli with phoA, stx1 and stx2 genes, respectively, when artificially inoculated minced beef and bovine faeces were tested. The LAMP calibration curves generated with pure cultures, and spiked beef and faeces, suggested that the assay had good quantification capability. Validation of the assay, performed using retail beef and bovine faeces samples, demonstrated good correlation between counts obtained by the LAMP assay and by a conventional culture method, but suggested the possibility of false negative LAMP results for 12.5-14.7% of samples tested. The multiplex LAMP assay developed potentially represents a rapid alternative to culture for monitoring E.coli levels in beef or faeces and it would provide additional information on the presence of VTEC. However, some further optimisation is needed to improve detection sensitivity.