Cooperative Proxying: An Approach to Reduce Network Energy Waste

A network connected host is expected to generate/respond to applications and protocols specific messages. Billions of Euro of electricity is wasted to keep idle hosts powered up 24/7 just to maintain network presence. This short paper describes the design of our cooperative Network Connectivity Proxy (NCP) that can impersonate sleeping hosts and responds to packets on their behalf as they were connected and fully operational. Thus, NCP is in fact an efficient approach to reduce network energy waste.

Integrase mutants defective for interaction with LEDGF/p75 are impaired in chromosome tethering and HIV-1 replication

The insertion of a DNA copy of its RNA genome into a chromosome of the host cell is mediated by the viral integrase with the help of mostly uncharacterized cellular cofactors. We have recently described that the transcriptional co-activator LEDGF/p75 strongly interacts with HIV-1 integrase. Here we show that interaction of HIV-1 integrase with LEDGF/p75 is important for viral replication. Using multiple approaches including two-hybrid interaction studies, random and directed mutagenesis, we could demonstrate that HIV-1 virus harboring a single mutation that disrupts integrase-LEDGF/p75 interaction, resulted in defective HIV-1 replication. Furthermore, we found that LEDGF/p75 tethers HIV-1 integrase to chromosomes and that this interaction may be important for the integration process and the replication of HIV-1.