Cell survival responses after exposure to modulated radiation fields.

In the present study survival responses were determined in cells with differing radiosensitivity, specifically primary fibroblast (AG0-1522B), human breast cancer (MDA-MB-231), human prostate cancer (DU-145) and human glioma (T98G) cells, after exposure to modulated radiation fields delivered by shielding 50% of the tissue culture flask. A significant decrease (P < 0.05) in cell survival was observed in the shielded area, outside the primary treatment field (out-of-field), that was lower than predicted when compared to uniform exposures fitted to the linear-quadratic model. Cellular radiosensitivity was demonstrated to be an important factor in the level of response for both the in- and out-of-field regions. These responses were shown to be dependent on secretion-mediated intercellular communication, because inhibition of cellular secreted factors between the in- and out-of-field regions abrogated the response. Out-of-field cell survival was shown to increase after pretreatment of cells with agents known to inhibit factors involved in mediating radiation-induced bystander signaling (aminoguanidine, DMSO or cPTIO). These data illustrate a significant decrease in survival out-of-field, dependent upon intercellular communication, in several cell lines with varying radiosensitivity after exposure to a modulated radiation field. This study provides further evidence for the importance of intercellular signaling in modulated exposures, where dose gradients are present, and may inform the refinement of established radiobiological models to facilitate the optimization of advanced radiotherapy treatment plans.

Effects of external axial magnetic field on fast electron propagation

A scheme employing an external axial magnetic field is proposed to diagnose the intrinsic divergence of laser-generated fast electron beams, and this is studied numerically with hybrid simulations. The maximum beam radius of fast electrons increases with the initial divergence and decreases with the amplitude of the axial magnetic field. It is indicated that the intrinsic divergence of fast electrons can be inferred from measurements of the beam radius at different depth under the axial field. The proposed scheme here may be useful for future fast ignition experiments and in other applications of laser-generated fast electron beams. (C) 2011 American Institute of Physics. [doi:10.1063/1.3630925]

Spectral modification of laser-accelerated proton beams by self-generated magnetic fields

Target normal measurements of proton energy spectra from ultrathin (50-200 nm) planar foil targets irradiated by 10(19) W cm(-2) 40 fs laser pulses exhibit broad maxima that are not present in the energy spectra from micron thickness targets (6 mu m). The proton flux in the peak is considerably greater than the proton flux observed in the same energy range in thicker targets. Numerical modelling of the experiment indicates that this spectral modification in thin targets is caused by magnetic fields that grow at the rear of the target during the laser-target interaction.

Temporally and spatially resolved measurements of multi-megagauss magnetic fields in high intensity laser-produced plasmas

We report spatially and temporally resolved measurements of self-generated multi-megagauss magnetic fields produced during ultrahigh intensity laser plasma interactions. Spatially resolved measurements of the magnetic fields show an asymmetry in the distribution of field with respect to the angle of laser incidence. Temporally resolved measurements of the self-generated third harmonic suggest that the strength of the magnetic field is proportional to the square root of laser intensity (i.e., the laser B-field) during the rise of the laser pulse. The experimental results are compared with numerical simulations using a particle-in-cell code which also shows clear asymmetry of the field profile and similar magnetic field growth rates and scalings.

Laboratory measurements of 0.7 GG magnetic fields generated during high-intensity laser interactions with dense plasmas

We report measurements of ultrahigh magnetic fields produced during intense (similar to10(20) Wcm(-2) mum(2)) laser interaction experiments with solids. We show that polarization measurements of high-order vuv laser harmonics generated during the interaction (up to the 15th order) suggest the existence of magnetic field strengths of 0.7+/-0.1 GG in the overdense plasma. Measurements using higher order harmonics indicate that denser regions of the plasma can be probed. This technique may be useful for measurements of multi-GG level magnetic fields which are predicted to occur at even higher intensities.