134 resultados para Fecal contamination indicators


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The detection of oxygen using optical sensors is of increasing interest, especially in modified atmosphere food packaging (MAP), in which the package, usually containing food, is flushed with a gas, such as carbon dioxide or nitrogen. This tutorial review examines the ideal properties of an oxygen optical sensor for MAP and compares them with those developed to date, including the most recent advances. The basic technologies underpinning the different indicator types are described, examples given and their potential for application in MAP assessed. This tutorial review should be of interest to the MAP industry and researchers in optical sensors and oxygen sensing.

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Zeranol, an oestrogenic growth promoter in food animals, is banned within the European Union (EU). However, commercially available immunoassay kits for zeranol cross-react with toxins formed by naturally occurring Fusarium spp. fungi, leading to false-positive screening results. This paper describes the validation of a specificity enhanced, rapid dry reagent time-resolved fluoroimmunoassay (TR-FIA) for zeranol (recovery 99%, limit of detection 1.3 ng ml(-1)) demonstrating that up to 150 ng ml(-1) of Fusarium spp. toxins in urine do not lead to false-positive results. This assay will assist EU Member States to implement Council Directive 961 23\EC, which requires states to monitor for potential abuses of zeranol. A similar TR-FIA for the Fusarium spp. toxin a-zearalenol, using the same sample extract, is also described (recovery 68%, limit of detection 5.6 ng ml(-1)). Only the addition of diluted sample extract is required to perform these dry-reagent TR-FIAs, the results being available within 1 h of extract application. The EU-funded project 'Natural Zeranol' (FAIR5-CT97-3443) will use these fluoroimmunoassays to screen bovine urine in four Member States to gather data on the seasonality of Fusarium spp. toxin contamination of urine and the incidence of zeranol screening test positives.

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Sustainable development comprises of three dimensions. The three dimensions are the environment, the social and the economic. There have been many indicators used to measure the three dimensions of sustainability. For example air pollution, consumption of natural resources, quality of open space, noise, equity and opportunities and economic benefits from transport and land use. Urban areas constitute the most crucial factor in the sustainability. Urban systems affect and are affected by natural systems beyond their physical boundaries and in general the interdependence between the urban system and the regional and global environment is not reflected in urban decision making. The use of energy in the urban system constitutes the major element in the construction and function of urban areas. Energy impacts across the boundaries of the three dimensions of sustainability. The objective of this research is to apply energy-use-indicators to the urban system as a measure of sustainability. This methodology is applied to a case study in the United Kingdom.