46 resultados para biological data
Filtro por publicador
- Repository Napier (1)
- ABACUS. Repositorio de Producción Científica - Universidad Europea (1)
- Aberdeen University (10)
- Aberystwyth University Repository - Reino Unido (5)
- Acceda, el repositorio institucional de la Universidad de Las Palmas de Gran Canaria. España (3)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (10)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (6)
- Applied Math and Science Education Repository - Washington - USA (1)
- Aquatic Commons (67)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (2)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (2)
- Aston University Research Archive (16)
- Avian Conservation and Ecology - Eletronic Cientific Hournal - Écologie et conservation des oiseaux: (1)
- Biblioteca de Teses e Dissertações da USP (1)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (22)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (9)
- Biblioteca Digital de la Universidad Católica Argentina (1)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (1)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (23)
- Boston University Digital Common (1)
- Brock University, Canada (5)
- Bucknell University Digital Commons - Pensilvania - USA (1)
- Bulgarian Digital Mathematics Library at IMI-BAS (4)
- CaltechTHESIS (2)
- Cambridge University Engineering Department Publications Database (12)
- CentAUR: Central Archive University of Reading - UK (15)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (3)
- Cochin University of Science & Technology (CUSAT), India (5)
- Collection Of Biostatistics Research Archive (3)
- CORA - Cork Open Research Archive - University College Cork - Ireland (5)
- Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest (2)
- Dalarna University College Electronic Archive (1)
- Deakin Research Online - Australia (43)
- Digital Commons - Michigan Tech (2)
- Digital Commons at Florida International University (7)
- Digital Peer Publishing (2)
- Digital Repository at Iowa State University (1)
- DigitalCommons - The University of Maine Research (1)
- DigitalCommons@The Texas Medical Center (13)
- DRUM (Digital Repository at the University of Maryland) (1)
- Duke University (16)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (12)
- Helda - Digital Repository of University of Helsinki (4)
- Indian Institute of Science - Bangalore - Índia (22)
- Institutional Repository of Leibniz University Hannover (1)
- Instituto Nacional de Saúde de Portugal (2)
- Martin Luther Universitat Halle Wittenberg, Germany (1)
- Memorial University Research Repository (2)
- National Center for Biotechnology Information - NCBI (26)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (28)
- Publishing Network for Geoscientific & Environmental Data (279)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (46)
- Queensland University of Technology - ePrints Archive (73)
- ReCiL - Repositório Científico Lusófona - Grupo Lusófona, Portugal (1)
- Repositório Científico da Universidade de Évora - Portugal (1)
- Repositório Digital da UNIVERSIDADE DA MADEIRA - Portugal (1)
- Repositório Institucional da Universidade de Aveiro - Portugal (2)
- Repositório Institucional da Universidade de Brasília (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (74)
- SAPIENTIA - Universidade do Algarve - Portugal (3)
- Universidad de Alicante (3)
- Universidad Politécnica de Madrid (12)
- Universidade de Lisboa - Repositório Aberto (3)
- Universidade Federal do Pará (4)
- Universita di Parma (1)
- Universitat de Girona, Spain (2)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (1)
- Université de Lausanne, Switzerland (3)
- Université de Montréal, Canada (3)
- University of Connecticut - USA (1)
- University of Michigan (2)
- University of Queensland eSpace - Australia (23)
- University of Southampton, United Kingdom (1)
- University of Washington (2)
Resumo:
Microneedle technology provides the opportunity for the delivery of DNA therapeutics by a non-invasive, patient acceptable route. To deliver DNA successfully requires consideration of both extra and intracellular biological barriers. In this study we present a novel two tier platform; i) a peptide delivery system, termed RALA, that is able to wrap the DNA into nanoparticles, protect the DNA from degradation, enter cells, disrupt endosomes and deliver the DNA to the nucleus of cells ii) a microneedle (MN) patch that will house the nanoparticles within the polymer matrix, breach the skin's stratum corneum barrier and dissolve upon contact with skin interstitial fluid thus releasing the nanoparticles into the skin. Our data demonstrates that the RALA is essential for preventing DNA degradation within the poly(vinylpyrrolidone) (PVP) polymer matrix. In fact the RALA/DNA nanoparticles (NPs) retained functionality when in the MN arrays after 28days and over a range of temperatures. Furthermore the physical strength and structure of the MNs was not compromised when loaded with the NPs. Finally we demonstrated the effectiveness of our MN-NP platform in vitro and in vivo, with systemic gene expression in highly vascularised regions. Taken together this 'smart-system' technology could be applied to a wide range of genetic therapies.