31 resultados para Public Transport Technologies
Filtro por publicador
- Repository Napier (1)
- Aberdeen University (2)
- Aberystwyth University Repository - Reino Unido (2)
- Academic Archive On-line (Karlstad University; Sweden) (2)
- Academic Archive On-line (Mid Sweden University; Sweden) (1)
- Academic Archive On-line (Stockholm University; Sweden) (1)
- Academic Research Repository at Institute of Developing Economies (1)
- Acceda, el repositorio institucional de la Universidad de Las Palmas de Gran Canaria. España (2)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (4)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (4)
- Aquatic Commons (3)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (1)
- Archive of European Integration (14)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (4)
- Aston University Research Archive (8)
- B-Digital - Universidade Fernando Pessoa - Portugal (1)
- Biblioteca de Teses e Dissertações da USP (2)
- Biblioteca Digital | Sistema Integrado de Documentación | UNCuyo - UNCUYO. UNIVERSIDAD NACIONAL DE CUYO. (3)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (2)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (1)
- Bioline International (1)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (12)
- Boston University Digital Common (1)
- Brock University, Canada (1)
- Bulgarian Digital Mathematics Library at IMI-BAS (1)
- Cambridge University Engineering Department Publications Database (5)
- CamPuce - an association for the promotion of science and humanities in African Countries (2)
- CentAUR: Central Archive University of Reading - UK (15)
- Cochin University of Science & Technology (CUSAT), India (3)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (48)
- CORA - Cork Open Research Archive - University College Cork - Ireland (6)
- Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest (2)
- Dalarna University College Electronic Archive (4)
- Deakin Research Online - Australia (70)
- Digital Commons @ DU | University of Denver Research (2)
- Digital Commons at Florida International University (4)
- Digital Peer Publishing (1)
- DigitalCommons@The Texas Medical Center (3)
- DigitalCommons@University of Nebraska - Lincoln (3)
- Doria (National Library of Finland DSpace Services) - National Library of Finland, Finland (1)
- DRUM (Digital Repository at the University of Maryland) (2)
- FUNDAJ - Fundação Joaquim Nabuco (1)
- Gallica, Bibliotheque Numerique - Bibliothèque nationale de France (French National Library) (BnF), France (15)
- Greenwich Academic Literature Archive - UK (3)
- Helda - Digital Repository of University of Helsinki (3)
- Indian Institute of Science - Bangalore - Índia (7)
- Institutional Repository of Leibniz University Hannover (1)
- Instituto Politécnico de Viseu (2)
- Instituto Politécnico do Porto, Portugal (2)
- Instituto Superior de Psicologia Aplicada - Lisboa (1)
- Lume - Repositório Digital da Universidade Federal do Rio Grande do Sul (1)
- Portal de Revistas Científicas Complutenses - Espanha (4)
- Publishing Network for Geoscientific & Environmental Data (1)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (31)
- Queensland University of Technology - ePrints Archive (240)
- RDBU - Repositório Digital da Biblioteca da Unisinos (1)
- Repositório Científico do Instituto Politécnico de Lisboa - Portugal (8)
- Repositório digital da Fundação Getúlio Vargas - FGV (11)
- Repositório Institucional da Universidade de Aveiro - Portugal (4)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (19)
- Royal College of Art Research Repository - Uninet Kingdom (1)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (7)
- SAPIENTIA - Universidade do Algarve - Portugal (1)
- Universidad de Alicante (2)
- Universidad del Rosario, Colombia (9)
- Universidad Politécnica de Madrid (46)
- Universidade de Lisboa - Repositório Aberto (1)
- Universidade Federal de Uberlândia (1)
- Universidade Federal do Pará (2)
- Universidade Federal do Rio Grande do Norte (UFRN) (8)
- Universidade Técnica de Lisboa (2)
- Universitat de Girona, Spain (1)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (5)
- Université de Lausanne, Switzerland (2)
- Université de Montréal (2)
- Université de Montréal, Canada (27)
- University of Canberra Research Repository - Australia (2)
- University of Michigan (178)
- University of Queensland eSpace - Australia (17)
- WestminsterResearch - UK (3)
Resumo:
Multidrug resistance arising from the activity of integral membrane transporter proteins presents a global public health threat. In bacteria such as Escherichia coli, transporter proteins belonging to the major facilitator superfamily make a considerable contribution to multidrug resistance by catalysing efflux of myriad structurally and chemically different antimicrobial compounds. Despite their clinical relevance, questions pertaining to mechanistic details of how these promiscuous proteins function remain outstanding, and the role(s) played by individual amino acid residues in recognition, binding and subsequent transport of different antimicrobial substrates by multidrug efflux members of the major facilitator superfamily requires illumination. Using in silico homology modelling, molecular docking and mutagenesis studies in combination with substrate binding and transport assays, we identified several amino acid residues that play important roles in antimicrobial substrate recognition, binding and transport by Escherichia coli MdtM, a representative multidrug efflux protein of the major facilitator superfamily. Furthermore, our studies suggested that 'aromatic clamps' formed by tyrosine and phenylalanine residues located within the substrate binding pocket of MdtM may be important for antimicrobial substrate recognition and transport by the protein. Such 'clamps' may be a structurally and functionally important feature of all major facilitator multidrug efflux proteins.