POU2F1 activity regulates HOXD10 and HOXD11 promoting a proliferative and invasive phenotype in head and neck cancer

HOX genes are master regulators of organ morphogenesis and cell differentiation during embryonic development, and continue to be expressed throughout post-natal life. To test the hypothesis that HOX genes are dysregulated in head and neck squamous cell carcinoma (HNSCC) we defined their expression profile, and investigated the function, transcriptional regulation and clinical relevance of a subset of highly expressed HOXD genes. Two HOXD genes, D10 and D11, showed strikingly high levels in HNSCC cell lines, patient tumor samples and publicly available datasets. Knockdown of HOXD10 in HNSCC cells caused decreased proliferation and invasion, whereas knockdown of HOXD11 reduced only invasion. POU2F1 consensus sequences were identified in the 5' DNA of HOXD10 and D11. Knockdown of POU2F1 significantly reduced expression of HOXD10 and D11 and inhibited HNSCC proliferation. Luciferase reporter constructs of the HOXD10 and D11 promoters confirmed that POU2F1 consensus binding sites are required for optimal promoter activity. Utilizing patient tumor samples a significant association was found between immunohistochemical staining of HOXD10 and both the overall and the disease-specific survival, adding further support that HOXD10 is dysregulated in head and neck cancer. Additional studies are now warranted to fully evaluate HOXD10 as a prognostic tool in head and neck cancers.

Control Strategues for Automatic Generation Control over MTDC Grid

Increasingly in power systems, there is a trend towards the sharing of reserves and integration of markets over wide areas in order to enable increased penetration of renewable sources in interconnected power systems. In this paper, a number of simple PI and gain based Model Predictive Control algorithms are proposed for Automatic Generation Control in AC areas connected to Multi-Terminal Direct Current grids. The paper discusses how this approach improves the sharing of secondary reserves and could assist in achieving EU energy targets for 2030 and beyond.

The Pilot Maths Centre at the North West Regional College

This paper discusses the establishment in 2015 of the first Maths Centre in any Further and Higher Education College in Northern Ireland. In particular, it considers the rationale for its development, how it was set up and how it runs. It also presents initial figures and feedback, and plans for future developments.