Solid-bound, proton-driven, fluorescent ‘off–on–off’ switches based on PET (photoinduced electron transfer)

Anthracene-based, H+-driven, ‘off–on–off’ fluorescent PET (photoinduced electron transfer) switches are immobilized on organic and inorganic polymeric solids in the form of Tentagel® and silica, respectively. The environment of the organic bead displaces apparent switching thresholds towards lower pH values whereas the Si–O- groups of silica electrostatically cause the opposite effect. These switches are ternary logic gate tags, one of which can be particularly useful in strengthening molecular computational identification (MCID) of small solid objects.

Highly CO2 sensitive extruded fluorescent plastic indicator film based on HPTS

Highly-sensitive optical fluorescent extruded plastic films are reported for the detection of gaseous and dissolved CO2. The pH-sensitive fluorescent dye used is 8-Hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS, PTS-) coated on the surface of hydrophilic fumed silica and the base is tetrabutylammonium hydroxide (TBAH). The above components are used to create an HPTS pigment (i.e. HPTS/SiO2/TBAH) with a high CO2 sensitivity (%CO2(S=1/2) = 0.16%) and fast 50% response (t50↓) = 2 s and recovery (t50↑) = 5 s times. Highly CO2-sensitive plastic films are then fabricated, via the extrusion of the HPTS pigment powder in low-density polyethylene (LDPE). As with the HPTS-pigment, the luminescence intensity (at 515 nm) and absorbance (at 475 nm) of the HPTS plastic film decreases as the %CO2 in the ambient gas phase increases. The HPTS plastic film exhibits a high CO2 sensitivity, %CO2(S=1/2), of 0.29%, but a response time ˂2 min and recovery time ˂40 min, which is slower than that of the HPTS pigment. The HPTS plastic film is very stable under ambient conditions, (with a shelf life ˃ six month when stored in the dark but under otherwise ambient conditions). Moreover, the HPTS-film is stable in water, salt solution and even in acid (pH=2), and in each of these media it can be used to detect dissolved CO2.

The uptake of silica during the spring bloom in the North East Atlantic Ocean

A full understanding of the biogeochemical cycling of silica in the North Atlantic is hampered by a lack of estimates of silica uptake by phytoplankton. We applied the ${}^{32}\text{Si}$ radiotracer incubation technique to determine silica uptake rates at 10 sites during the UK-(Natural Environment Research Council) Faroes-Iceland-Scotland hydrographic and environmental survey (FISHES) cruise in the Northeast Atlantic, May 2001. Column silica uptake rates ranged between 6 and 166 mmol Si $\text{m}^{-2}\ \text{d}^{-1}$; this data set was integrated with concurrent hydrographic, chemical, and primary productivity data to explain these changes in silica uptake in terms of the progress of the spring bloom. In order to interpret data covering a relatively large spatial and temporal scale, we used mean photic zone silica concentration as a proxy time-series measure of diatom bloom progression. Both absolute and specific silica uptake rates were highest at dissolved silica concentrations >2 mmol $\text{L}^{-1}$. Si and C uptake were vertically decoupled at those stations where surface silica was strongly depleted. Absolute primary productivity was not strongly correlated with dissolved silica concentrations, owing to either exhaustion of silica at diatom-dominated stations or to dominance of the community by other phytoplankton. Silica uptake as a function of increased substrate concentration was linear up to 25 $\mu \text{mol}\ \text{L}^{-1}$; we consider some possible reasons for the nonhyperbolic response.

[Rh-2(COD)(2)(Dppm)(mu(2)-Cl)]BF4: Precursor for a selective hydrogenation catalyst and its recycling by silica entrapment

The synthesis of [Rh-2(COD)(2)(dppm)(mu(2)-Cl)] BF4 (1) (COD) 1,5-cyclooctadiene, dppm) bis(diphenylphosphino) methane) from simple precursors is reported. This is a rare example of a dirhodium complex with an open [Rh-2(mu(2)-dppm)(mu(2)-Cl)] core. The complex has been used to affect the hydrogenation of styrene and benzo[b] thiophene with total selectivity and competitive rates of reaction. The recycling of the catalyst has been achieved by the entrapment of 1 in silica by a sol-gel method to produce a recyclable solid catalyst.

Statistical modelling of cement slurries for self compacting SIFCON containing silica fume

This paper reports a study carried out to develop a self-compacting fibre reinforced concrete containing a high fibre content with slurry infiltrated fibre concrete (SIFCON). The SIFCON was developed with 10% of steel fibres which are infiltrated by self-compacting cement slurry without any vibration. Traditionally, the infiltration of the slurry into the layer of fibres is carried out under intensive vibration. A two-level fractional factorial design was used to optimise the properties of cement-based slurries with four independent variables, such as dosage of silica fume, dosage of superplasticiser, sand content, and water/cement ratio (W/C). Rheometer, mini-slump test, Lombardi plate cohesion meter, J-fibre penetration test, and induced bleeding were used to assess the behaviour of fresh cement slurries. The compressive strengths at 7 and 28 days were also measured. The statistical models are valid for slurries made with W/C of 0.40 to 0.50, 50 to 100% of sand by mass of cement, 5 to 10% of silica fume by mass of cement, and SP dosage of 0.6 to 1.2% by mass of cement. This model makes it possible to evaluate the effect of individual variables on measured parameters of fresh cement slurries. The proposed models offered useful information to understand trade-offs between mix variables and compare the responses obtained from various test methods in order to optimise self-compacting SIFCON.

Detection of a single DNA base-pair mismatch using an anthracene-tagged fluorescent probe

A novel anthracene-tagged oligonucleotide can discriminate between a fully-matched DNA target sequence and one with a single mismatching base-pair through a remarkable difference in fluorescence emission intensity upon duplex formation.