The Capital Sculpture of Wells Cathedral, c. 1184-1210

The understudied capital sculpture of Wells Cathedral in Somerset, England (c. 1184-1210) provides ample opportunity of expanding the current scholarship and understanding of interior ecclesiastical sculpture in a West Country cathedral. While the Gothic style of architecture is typically understood as, according to Paul Binski (2014), rational in execution and reception, the capital sculpture at Wells Cathedral has been considered illogical in terms of both its iconography and location within the nave, transepts, and north porch. Utilizing Michael Camille’s post/anti-iconographical approach, this project examines the Wells figural capitals in five case studies: labour, Old and New Testament Scenes, animals and beast fables, busts, and monsters and hybrids. Each group of capitals will be approached with an understanding that this type of art was viewed by people of different classes and professions, with each viewer bringing their own personal experiences and abilities into how they could have read and understood these types of images. Therefore, the capitals at Wells must be read through layers of meaning and interpretation while also considering their locations within the cathedral and how they react and respond to surrounding figural capitals.

The roles of EFN-1 and seam cell V2 in the transmission of a stop cue for the posterior lateral microtubule in Caenorhabditis elegans

A functional nervous system requires the precise arrangement of all nerve cells and their neurites. To achieve this correct assembly, a myriad of molecular guidance cues works together to direct the outgrowth of neurites to their correct positions. The small nematode C. elegans provides the ideal model system to study the complex mechanisms of neurite guidance due to its relatively simple nervous system, composed of 302 neurons. I used two mechanosensory neurons, called the posterior lateral microtubule (PLM), to investigate the role of the ephrin and Eph receptor protein family in neurite termination in C. elegans. Activation of the C. elegans Eph receptor VAB-1 on the PLM growth cone is sufficient to cause PLM termination, but the identity and location of the activating ligand has not been established. In my thesis I investigated the ability of the ephrin ligand EFN-1 to activate VAB-1 to cause PLM termination when expressed on the same cell (in cis) and on opposing cells (in trans) to the receptor. I showed that EFN-1 is able to activate VAB-1 in cis and in trans to cause PLM termination. I also assessed the hypodermal seam cells as the source of the ephrin stop cue using fluorescently labelled and seam cell mutant transgenic worms. I found that although the PLM shows consistent termination on the seam cell V2 in wild type worms independent of PLM length, this process is not significantly disrupted in seam cell mutants. With this information I have created a new hypothesis that the PLM neurite is able the provide a positional cue for the developing seam cells, and have created a new transgenic strain which can be used to assess the impact of PLM and ALM cell ablation on seam cell position. My research is the first to demonstrate the ability of an ephrin ligand to activate its ephrin receptor in cis, and further research can investigate if this finding has in vivo applications.