1 resultado para On the Origin of Species
em QSpace: Queen's University - Canada
Filtro por publicador
- Repository Napier (1)
- Aberdeen University (1)
- Acceda, el repositorio institucional de la Universidad de Las Palmas de Gran Canaria. España (5)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (1)
- Applied Math and Science Education Repository - Washington - USA (1)
- Aquatic Commons (54)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (2)
- Archimer: Archive de l'Institut francais de recherche pour l'exploitation de la mer (1)
- Archive of European Integration (21)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (2)
- Aston University Research Archive (11)
- Avian Conservation and Ecology - Eletronic Cientific Hournal - Écologie et conservation des oiseaux: (1)
- Biblioteca de Teses e Dissertações da USP (1)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (24)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (11)
- Biblioteca Digital de la Universidad Católica Argentina (1)
- Biodiversity Heritage Library, United States (5)
- Bioline International (7)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (29)
- Boston University Digital Common (1)
- Brock University, Canada (1)
- Bucknell University Digital Commons - Pensilvania - USA (1)
- Bulgarian Digital Mathematics Library at IMI-BAS (2)
- CaltechTHESIS (2)
- Cambridge University Engineering Department Publications Database (1)
- CentAUR: Central Archive University of Reading - UK (26)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (47)
- Cochin University of Science & Technology (CUSAT), India (20)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (1)
- CORA - Cork Open Research Archive - University College Cork - Ireland (4)
- Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest (5)
- Dalarna University College Electronic Archive (1)
- Deakin Research Online - Australia (25)
- Digital Archives@Colby (1)
- Digital Commons - Montana Tech (1)
- Digital Commons at Florida International University (9)
- DigitalCommons - The University of Maine Research (1)
- DigitalCommons@The Texas Medical Center (3)
- DigitalCommons@University of Nebraska - Lincoln (4)
- Digitale Sammlungen - Goethe-Universität Frankfurt am Main (1)
- Diposit Digital de la UB - Universidade de Barcelona (1)
- DRUM (Digital Repository at the University of Maryland) (2)
- Duke University (3)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (7)
- Helda - Digital Repository of University of Helsinki (4)
- Illinois Digital Environment for Access to Learning and Scholarship Repository (1)
- Indian Institute of Science - Bangalore - Índia (27)
- Instituto Politécnico de Bragança (1)
- Instituto Politécnico do Porto, Portugal (2)
- Memorial University Research Repository (1)
- Ministerio de Cultura, Spain (2)
- National Center for Biotechnology Information - NCBI (22)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (19)
- Portal de Revistas Científicas Complutenses - Espanha (1)
- Publishing Network for Geoscientific & Environmental Data (18)
- QSpace: Queen's University - Canada (1)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (27)
- Queensland University of Technology - ePrints Archive (221)
- Repositório Alice (Acesso Livre à Informação Científica da Embrapa / Repository Open Access to Scientific Information from Embrapa) (2)
- Repositório Científico da Universidade de Évora - Portugal (5)
- Repositório Científico do Instituto Politécnico de Lisboa - Portugal (1)
- Repositório digital da Fundação Getúlio Vargas - FGV (1)
- Repositório Institucional da Universidade de Aveiro - Portugal (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (105)
- SAPIENTIA - Universidade do Algarve - Portugal (8)
- Universidad de Alicante (8)
- Universidad Politécnica de Madrid (9)
- Universidade do Algarve (1)
- Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP) (5)
- Universidade Federal do Pará (3)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (2)
- Université de Lausanne, Switzerland (1)
- Université de Montréal, Canada (1)
- University of Michigan (91)
- University of Queensland eSpace - Australia (16)
- University of Washington (1)
Resumo:
Every aerobic organism expresses cytochrome c oxidase to catalyze reduction of molecular oxygen to water, and takes advantage of this energy releasing reaction to produce an electrochemical gradient used in cellular energy production. The protein SCO (Synthesis of cytochrome c oxidase) is a required assembly factor for the oxidase, conserved across many species. SCO is implicated in the assembly of one of two copper centres (ie., CuA) of cytochrome oxidase. The exact mechanism of SCO’s participation in CuA assembly is not known. SCO has been proposed to bind and deliver copper, or alternatively to act in reductive preparation of the CuA site within the oxidase. In this body of work, the strength and stability of Cu(II) binding to Bacillus subtilis SCO is explored via electronic absorption and fluorescence spectroscopies and by calorimetric methods. An equilibrium dissociation constant (Kd) of 3.5x10-12 M was determined as an upper limit for the BsSCO-Cu(II) interaction, via differential scanning calorimetry. In the first reported case for a SCO homolog, dissociation kinetics of Cu(II) from BsSCO were characterized, and found to be dependent on both ionic strength and the presence of free Cu(II) in solution. Further differential scanning calorimetry experiments performed at high ionic strength support a two-step model of BsSCO and Cu(II) binding. The implications of this model for the BsSCO-Cu(II) interaction are presented in relation to the mechanism of interaction between SCO and the CuA site of cytochrome c oxidase.