Simulating CO2 leakage into marine environments

Jerry Blackford of the Plymouth Marine Laboratory leads the UK Research Council funded Quantifying and Monitoring Potential Ecosystem Impacts of Geological Carbon Storage (QICS) program, and is a founding member of the new UK CCS Research Centre leading the environment research team. Here he talks to Muriel Cozier about how the world's first experiment to simulate a CO2 leak from underground storage in a marine environment will go a long way toward improving our understanding of a series of complex interactions.

2015 David Cushing Prize

The prize honors the memory of David Cushing, Founding Editor of Journal of Plankton Research. It is awarded annually for the best paper by an early career stage scientist published in the journal during the previous year. The prize helps foster the interesting and high-quality papers by young scientists that David Cushing so actively supported. The 2015 David Cushing Prize has been awarded to Bingzhang Chen for his paper, “Patterns of thermal limits of phytoplankton” (J. Plankton Res. 37, 285–292) Bingzhang Chen obtained his PhD with a major in Marine Environmental Science at the Hong Kong University of Science and Technology under the supervision of Dr Hongbin Liu in 2008. He also worked with Dr Zoe Finkel and Dr Andrew Irwin in Mount Allison University, Canada, from 2009 to 2010. During this time, he started to learn the R language and entered the field of data analysis and programming. This experience has been proved very useful for his later work.

2015 David Cushing Prize

The prize honors the memory of David Cushing, Founding Editor of Journal of Plankton Research. It is awarded annually for the best paper by an early career stage scientist published in the journal during the previous year. The prize helps foster the interesting and high-quality papers by young scientists that David Cushing so actively supported. The 2015 David Cushing Prize has been awarded to Bingzhang Chen for his paper, “Patterns of thermal limits of phytoplankton” (J. Plankton Res. 37, 285–292) Bingzhang Chen obtained his PhD with a major in Marine Environmental Science at the Hong Kong University of Science and Technology under the supervision of Dr Hongbin Liu in 2008. He also worked with Dr Zoe Finkel and Dr Andrew Irwin in Mount Allison University, Canada, from 2009 to 2010. During this time, he started to learn the R language and entered the field of data analysis and programming. This experience has been proved very useful for his later work.

DNA from historical and trophy samples provides insights into white shark population origins and genetic diversity

Characterizing genetic variation by retrospective genotyping of trophy or historical artifacts from endangered species is an important conservation tool. Loss of genetic diversity in top predators such as the white shark Carcharodon carcharias remains an issue, exacerbated in this species by declining, sometimes isolated philopatric populations. We successfully sequenced mitochondrial DNA (mtDNA) D-loop from osteodentine of contemporary South African white shark teeth (from 3 jaws), and from 34 to 129 yr old dried cartilage and skin samples from 1 Pacific Ocean and 5 Mediterranean sharks. Osteodentine-derived sequences from South African fish matched those derived from an individual’s finclips, but were generally of poorer quality than those from skin and cartilage of historical samples. Three haplotypes were identified from historical Mediterranean samples (n = 5); 2 individuals had unique sequences and 3 shared the contemporary Mediterranean haplotype. Placement of previously undescribed mtDNA haplotypes from historical material within both the Mediterranean and Pacific clades fits with the accepted intra-specific phylogeny derived from contemporary material, verifying our approaches. The utility of our methodology is in its provision of additional genetic resources from osteodentine (for species lacking tooth pulp) and cartilage of rare and endangered species held in often uncurated, contemporary and historical dry collections. Such material can usefully supplement estimates of connectivity, population history, and stock viability. We confirm the depauperate haplotype diversity of historical Mediterranean sharks, consistent with founding by a small number of Pacific colonizers. The consequent lack of diversity suggests serious challenges for the maintenance of this top predator and the Mediterranean ecosystem.

DNA from historical and trophy samples provides insights into white shark population origins and genetic diversity

Characterizing genetic variation by retrospective genotyping of trophy or historical artifacts from endangered species is an important conservation tool. Loss of genetic diversity in top predators such as the white shark Carcharodon carcharias remains an issue, exacerbated in this species by declining, sometimes isolated philopatric populations. We successfully sequenced mitochondrial DNA (mtDNA) D-loop from osteodentine of contemporary South African white shark teeth (from 3 jaws), and from 34 to 129 yr old dried cartilage and skin samples from 1 Pacific Ocean and 5 Mediterranean sharks. Osteodentine-derived sequences from South African fish matched those derived from an individual’s finclips, but were generally of poorer quality than those from skin and cartilage of historical samples. Three haplotypes were identified from historical Mediterranean samples (n = 5); 2 individuals had unique sequences and 3 shared the contemporary Mediterranean haplotype. Placement of previously undescribed mtDNA haplotypes from historical material within both the Mediterranean and Pacific clades fits with the accepted intra-specific phylogeny derived from contemporary material, verifying our approaches. The utility of our methodology is in its provision of additional genetic resources from osteodentine (for species lacking tooth pulp) and cartilage of rare and endangered species held in often uncurated, contemporary and historical dry collections. Such material can usefully supplement estimates of connectivity, population history, and stock viability. We confirm the depauperate haplotype diversity of historical Mediterranean sharks, consistent with founding by a small number of Pacific colonizers. The consequent lack of diversity suggests serious challenges for the maintenance of this top predator and the Mediterranean ecosystem.