Distribution, occurrence and biotoxin composition of the main shellfish toxin producing microalgae within European waters: A comparison of methods of analysis.

Harmful algal blooms (HABs) are a natural global phenomena emerging in severity and extent. Incidents have many economic, ecological and human health impacts. Monitoring and providing early warning of toxic HABs are critical for protecting public health. Current monitoring programmes include measuring the number of toxic phytoplankton cells in the water and biotoxin levels in shellfish tissue. As these efforts are demanding and labour intensive, methods which improve the efficiency are essential. This study compares the utilisation of a multitoxin surface plasmon resonance (multitoxin SPR) biosensor with enzyme-linked immunosorbent assay (ELISA) and analytical methods such as high performance liquid chromatography with fluorescence detection (HPLC-FLD) and liquid chromatography–tandem mass spectrometry (LC–MS/MS) for toxic HAB monitoring efforts in Europe. Seawater samples (n = 256) from European waters, collected 2009–2011, were analysed for biotoxins: saxitoxin and analogues, okadaic acid and dinophysistoxins 1/2 (DTX1/DTX2) and domoic acid responsible for paralytic shellfish poisoning (PSP), diarrheic shellfish poisoning (DSP) and amnesic shellfish poisoning (ASP), respectively. Biotoxins were detected mainly in samples from Spain and Ireland. France and Norway appeared to have the lowest number of toxic samples. Both the multitoxin SPR biosensor and the RNA microarray were more sensitive at detecting toxic HABs than standard light microscopy phytoplankton monitoring. Correlations between each of the detection methods were performed with the overall agreement, based on statistical 2 × 2 comparison tables, between each testing platform ranging between 32% and 74% for all three toxin families illustrating that one individual testing method may not be an ideal solution. An efficient early warning monitoring system for the detection of toxic HABs could therefore be achieved by combining both the multitoxin SPR biosensor and RNA microarray.

Distribution, occurrence and biotoxin composition of the main shellfish toxin producing microalgae within European waters: A comparison of methods of analysis.

Harmful algal blooms (HABs) are a natural global phenomena emerging in severity and extent. Incidents have many economic, ecological and human health impacts. Monitoring and providing early warning of toxic HABs are critical for protecting public health. Current monitoring programmes include measuring the number of toxic phytoplankton cells in the water and biotoxin levels in shellfish tissue. As these efforts are demanding and labour intensive, methods which improve the efficiency are essential. This study compares the utilisation of a multitoxin surface plasmon resonance (multitoxin SPR) biosensor with enzyme-linked immunosorbent assay (ELISA) and analytical methods such as high performance liquid chromatography with fluorescence detection (HPLC-FLD) and liquid chromatography–tandem mass spectrometry (LC–MS/MS) for toxic HAB monitoring efforts in Europe. Seawater samples (n = 256) from European waters, collected 2009–2011, were analysed for biotoxins: saxitoxin and analogues, okadaic acid and dinophysistoxins 1/2 (DTX1/DTX2) and domoic acid responsible for paralytic shellfish poisoning (PSP), diarrheic shellfish poisoning (DSP) and amnesic shellfish poisoning (ASP), respectively. Biotoxins were detected mainly in samples from Spain and Ireland. France and Norway appeared to have the lowest number of toxic samples. Both the multitoxin SPR biosensor and the RNA microarray were more sensitive at detecting toxic HABs than standard light microscopy phytoplankton monitoring. Correlations between each of the detection methods were performed with the overall agreement, based on statistical 2 × 2 comparison tables, between each testing platform ranging between 32% and 74% for all three toxin families illustrating that one individual testing method may not be an ideal solution. An efficient early warning monitoring system for the detection of toxic HABs could therefore be achieved by combining both the multitoxin SPR biosensor and RNA microarray.

Surface water subduction during a downwelling event in a semi-enclosed bay

The Ría de Vigo is a bay strongly influenced by upwelling-downwelling cycles along the adjacent coast of NW Iberia. Moored and ship-board observations during September 2006 showed that subduction, initially associated with an estuarine circulation, strengthened when a strong downwelling circulation, resulting from northward wind over the coastal ocean, was generated in the outer ría causing ambient waters to be advected outward in the lower layer. Incoming surface waters confined the estuarine circulation to the shallow interior and displaced isopleths downward through the water column at ∼10 m d−1. As the estuarine circulation retreated inward, strong flow convergence developed between middle and inner ria in the layer above 15 m, while divergence developed beneath. The convergence increased through the period of downwelling-favorable wind at a rate consistent with the observed isopleth displacement velocities. The coefficient of turbulent diffusion Kt, from a microstructure profiler, indicated that mixing was strong in the estuarine circulation and subsequently in the downwelling zone, where localized instabilities and temperature-salinity inversions were observed. During the downwelling, concentrations of phytoplankton, including potentially harmful species, increased, especially in the middle and inner ria, as a result of inward advection, subduction and the ability of the dinoflagellates to maintain their position in the water column by swimming. In the course of the 5 day event, the water mass of all but the innermost ría was flushed completely and replaced by waters originating in the coastally-trapped poleward flow along the Atlantic coastline.

Surface water subduction during a downwelling event in a semi-enclosed bay

The Ría de Vigo is a bay strongly influenced by upwelling-downwelling cycles along the adjacent coast of NW Iberia. Moored and ship-board observations during September 2006 showed that subduction, initially associated with an estuarine circulation, strengthened when a strong downwelling circulation, resulting from northward wind over the coastal ocean, was generated in the outer ría causing ambient waters to be advected outward in the lower layer. Incoming surface waters confined the estuarine circulation to the shallow interior and displaced isopleths downward through the water column at ∼10 m d−1. As the estuarine circulation retreated inward, strong flow convergence developed between middle and inner ria in the layer above 15 m, while divergence developed beneath. The convergence increased through the period of downwelling-favorable wind at a rate consistent with the observed isopleth displacement velocities. The coefficient of turbulent diffusion Kt, from a microstructure profiler, indicated that mixing was strong in the estuarine circulation and subsequently in the downwelling zone, where localized instabilities and temperature-salinity inversions were observed. During the downwelling, concentrations of phytoplankton, including potentially harmful species, increased, especially in the middle and inner ria, as a result of inward advection, subduction and the ability of the dinoflagellates to maintain their position in the water column by swimming. In the course of the 5 day event, the water mass of all but the innermost ría was flushed completely and replaced by waters originating in the coastally-trapped poleward flow along the Atlantic coastline.