Sexism and Imperialism in Mozart's Don Giovanni, Puccini's Madama Butterfly, and Blitzstein's Regina: A Performance Study

This dissertation project comprises three major operatic performances and an accompanying document; a performance study which surveys aspects of sexism and imperialism as represented in three operas written over the last three centuries by examining the implications of prejudice through research as well as through performances of the major roles found in the operas. Mr. Eversole performed the role of Sharpless in the 2014 Castleton Festival production of Madama Butterfly (music by Giacomo Puccini, libretto by Luigi Illica and Giuseppe Giacosa), conducted by Bradley Moore. In 2015, Mr. Eversole sang the title role in four performances of Mozart and Da Ponte’s Don Giovanni with the Maryland Opera Studio at the Clarice Smith Performing Arts Center, conducted by Craig Kier. Also as part of the Maryland Opera Studio 2015-16 season, Mr. Eversole appeared as Oscar Hubbard in four performances of Marc Blitzstein’s Regina, an adaptation of Lillian Hellman’s 1939 play, The Little Foxes. These performances were also conducted by Craig Kier. The accompanying research document discusses significant issues of cultural, geographical, and sexual hegemony as they relate to each opera. It examines the plots and characters of the operas from a postcolonial and feminist perspective, and takes a moral stance against imperialism, sexism, domestic abuse, and in general, the exploitation of women and of the colonized by the socially privileged and powerful. Recordings of all three operas can be accessed at the University of Maryland Hornbake Library. They are: Giacomo Puccini’s Madama Butterfly (the role of Sharpless) July 20, 2014, Castleton Festival production, Bradley Moore, Conductor Castleton, Virginia Wolfgang Amadeus Mozart’s Don Giovanni (title role) November 22nd, 2015, Maryland Opera Studio, Craig Kier, Conductor Clarice Smith Performing Arts Center, UMD Marc Blitzstein’s Regina, (Oscar Hubbard) April 8th, 8016, Maryland Opera Studio, Craig Kier, Conductor Clarice Smith Performing Arts Center, UMD

Drug Delivery through the Blood-Brain Barrier through Use of Vascular Cell Adhesion Molecule 1- Targeted Nanoparticles

The blood brain barrier (BBB) is a semi-permeable membrane separating the brain from the bloodstream, preventing many drugs that treat neurological diseases, such as Alzheimer’s and Parkinson’s, from reaching the brain. Our project aimed to create a novel drug delivery system targeting the brain during neural inflammation. We developed a cationic solid lipid nanoparticle (CSLN) complex composed of cationic nanoparticles, biotin, streptavidin, and anti-vascular cell adhesion molecule-1 (anti- VCAM-1) antibodies. The anti-VCAM-1 antibody is used to target VCAM-1, a cell adhesion protein found on the BBB endothelium. VCAM-1 expression is elevated in the presence of inflammatory molecules, such as tumor necrosis factor-alpha (TNF- α). Through the use of a simple BBB model, results showed that our novel drug delivery system experienced some level of success in targeting the brain inflammation due to increasing TNF-α concentrations. This is promising for drug delivery research and provides support for VCAM-1 targeting using more robust and complex BBB models.