ATTACHMENT, GROWTH AND PERSISTENCE OF CRONOBACTER ON GRANULAR ACTIVATED CARBON FILTERS

Several Cronobacter outbreaks have implicated contaminated drinking water. This study assessed the impact of granular activated carbon (GAC) on the microbial quality of the water produced. A simulated water filter system was installed by filling plastic columns with sterile GAC, followed by sterile water with a dilute nutrient flowing through the column at a steady rate. Carbon columns were inoculated with Cronobacter on the surface, and the effluent monitored for Cronobacter levels. During a second phase, commercial faucet filters were distributed to households for 4-month use. Used filters were backwashed with sterile peptone water, and analyzed for Cronobacter, total aerobic plate count, coliform bacteria and Enterobacteriaceae. Cronobacter colonized the simulated GAC and grew when provided minimal levels of nutrients. Backwashed used filters used in home settings yielded presumptive Escherichia coli, Pseudomonas and other waterborne bacteria. Presumptive Cronobacter strains were identified as negative through biochemical and genetic test.

Water Quality Impacts due to the Addition of Biosolids-Derived Compost to Bioretention

Bioretention is a common stormwater control measure (SCM). While compost, combined with other bioretention soil media (BSM), has the potential for increased pollutant and water uptake and storage, it also may leach harmful nutrients. Limited information is available on the use of compost in SCMs. Therefore, this project seeks to analyze the impacts of the addition of biosolids-derived compost to bioretention. To accomplish this, bioretention mesocosm column studies were conducted to determine the leaching effects of 15%, 30%, and 30% tap water-washed compost, mixed with standard BSM. Synthetic storm runoff was applied to the columns and the effluent was analyzed for total nitrogen (N), phosphorus (P), and their speciation. All three columns leached N and P with maximum total N concentrations of 2,200, 2,100, and 300 mg-N/L and total P concentrations of 12, 4.9, and 4.6 mg-P/L for the 30%, 15%, and 30% washed mesocosms, respectively. Therefore, based on this study, it is not recommended that biosolids-derived compost be added to bioretention media.