Dinner Prelude to the Nebraska Great Gardens Symposium

It’s a pleasure to welcome you to the University of Nebraska here in Lincoln. I’m delighted to be asked to join you for this dinner prelude to your Nebraska Great Gardens Symposium tomorrow. I’m also pleased to be able to welcome you to East Campus tonight, and to the Institute of Agriculture and Natural Resources, and I understand you’ll be meeting at the City Campus Union tomorrow. I am glad you have the opportunity to visit both campuses, and I hope you will come back when they are in bloom. As a newcomer to Nebraska myself – my wife Virginia and I arrived on a cold, snowy day last December, and I began my position here January 2 – I’m certainly looking forward to the arrival of spring. I have been told what a joy it is to be on this campus when plants and trees are in bloom, and when that special sense of spring renewal surrounds us.

The Nebraska State Grange

Thank you so much for inviting my wife Virginia and me to be with you today. It delights me to talk about land-grant universities in general, and about the land-grant university mission we take so very seriously in the Institute of Agriculture and Natural Resources in particular, because I am so proud of the way our faculty and staff continually strive to contribute to and improve the economic and societal well-being of rural Nebraska, as well as all of Nebraska.

Development of an Electrochemical Insulin Sensor Based on a High Affinity DNA Sequence Found in the Insulin-linked Polymorphic Region

The detection of pertinent biomarkers has the potential provide an early indication of disease progression before considerable damage has been incurred. A decrease in an individual’s sensitivity to insulin, which may be quantified as the ratio of insulin to glucose in the blood after a glucose pulse, has recently been reported as an early predictor of insulin-dependent diabetes mellitus. Routine measurement of insulin levels is therefore desirable in the care of diabetes-prone individuals. A rapid, simple, and reagentless method for insulin detection would allow for wide-spread screenings that provide earlier signs of diabetes onset. The aim of this thesis is to develop a folding-base electrochemical sensor for the detection of insulin. The sensor described herein consists of a DNA probe immobilized on a gold disc electrode via an alkanethiol linker and embedded in an alkanethiol self-assembled monolayer. The probe is labeled with a redox reporter, which readily transfers electrons to the gold electrode in the absence of insulin. In the presence of insulin, electron transfer is inhibited, presumably due to a binding-induced conformational or dynamic change in the DNA probe that significantly alters the electron-tunneling pathway. A 28-base segment of the insulin-linked polymorphic region that has been reported to bind insulin with high affinity serves as the capture element of the DNA probe. Three probe constructs that vary in their secondary structure and position of the redox label are evaluated for their utility as insulin-sensing elements on the electrochemical platform. The effects of probe modification on secondary structure are also evaluated using circular dichroism spectroscopy.