Development and application of DNA techniques for validating and improving pinniped diet estimates

Polymerase chain reaction techniques were developed and applied to identify DNA from .40 species of prey contained in fecal (scat) soft-part matrix collected at terrestrial sites used by Steller sea lions (Eumetopias jubatus) in British Columbia and the eastern Aleutian Islands, Alaska. Sixty percent more fish and cephalopod prey were identified by morphological analyses of hard parts compared with DNA analysis of soft parts (hard parts identified higher relative proportions of Ammodytes sp., Cottidae, and certain Gadidae). DNA identified 213 prey occurrences, of which 75 (35%) were undetected by hard parts (mainly Salmonidae, Pleuronectidae, Elasmobranchii, and Cephalopoda), and thereby increased species occurrences by 22% overall and species richness in 44% of cases (when comparing 110 scats that amplified prey DNA). Prey composition was identical within only 20% of scats. Overall, diet composition derived from both identification techniques combined did not differ significantly from hard-part identification alone, suggesting that past scat-based diet studies have not missed major dietary components. However, significant differences in relative diet contributions across scats (as identified using the two techniques separately) reflect passage rate differences between hard and soft digesta material and highlight certain hypothesized limitations in conventional morphological-based methods (e.g., differences in resistance to digestion, hard part regurgitation, partial and secondary prey consumption), as well as potential technical issues (e.g., resolution of primer efficiency and sensitivity and scat subsampling protocols). DNA analysis of salmon occurrence (from scat soft-part matrix and 238 archived salmon hard parts) provided species-level taxonomic resolution that could not be obtained by morphological identification and showed that Steller sea lions were primarily consuming pink (Oncorhynchus gorbuscha) and chum (Oncorhynchus keta) salmon. Notably, DNA from Atlantic salmon (Salmo salar) that likely originated from a distant fish farm was also detected in two scats from one site in the eastern Aleutian Islands. Overall, molecular techniques are valuable for identifying prey in the fecal remains of marine predators. Combining DNA and hard-part identification will effectively alleviate certain predicted biases and will ultimately enhance measures of diet richness, fisheries interactions (especially salmon-related ones), and the ecological role of pinnipeds and other marine predators, to the benefit of marine wildlife conservationists and fisheries managers.

Information and Communication Technologies for Educational Development: The Case of Cybercafés at Obafemi Awolowo University, Ile-Ife, Nigeria

This study examines the use of Cybercafés/Internet resources and the evaluation of their usefulness. About eight Cybercafés located in the university community were used in this study. Questionnaires, interviews with the Cybercafé owners, staff and users as well as personal observations made during inspection of these cafés were used in this study. The data were analysed according to the background of the Internet users. The richness and high speed, accuracy, and authority were used by users to judge the quality of the Internet. Information such as the establishment of the café's facilities, membership and the future of the Cybercafés were also looked into. Finally, one can clearly see that the dominating impact of digital technology has crossed the Rubicon of controversy. The result of the survey shows that forty percent of the users learnt to use the internet by self instruction, thirty five percent learnt from colleagues or friends. Those in the sciences use the internet the most, the channel mostly used in obtaining information is the search engines. A large number of students, faculties and researchers make use of the internet in obtaining information. Many of those of those users make use of the Cybercafés in the university community.