Host Specificity of Calyptospora funduli (Apicomplexa: Calyptosporidae) in Atheriniform Fishes

Calyptospora funduli has a broad host specificity, infecting at least 7 natural and 10 additional experimental definitive hosts, all atheriniform fishes within 5 families, but most in the genus Fundulus. Barriers, apparently innate ones, prevent any development of C. funduli in perciform fishes but allow incomplete or abnormal development of the parasite in a few unnatural atheriniform hosts. In the freshwater species Fundulus olivaceus and Fundulus notti, these abnormalities consisted of asynchronous development, degeneration of the parasite in early stages of development, and the formation of numerous macrophage aggregates. Rivulus marmoratus has the ability to eliminate infections with a granulomatous inflammatory response. Additional barriers that limit natural infections of C. funduli in other hosts include feeding behavior, environmental conditions, and geographic isolation.

Survivable Waveband Switching in WDM Mesh Networks under Dedicated Path-Protection

This paper considers the problem of dedicated path-protection in wavelength-division multiplexed (WDM) mesh networks with waveband switching functionality under shared risk link group (SRLG) constraints. Two dedicated path protection schemes are proposed, namely the PBABL scheme and the MPABWL scheme. The PBABL scheme protects each working waveband-path through a backup waveband-path. The MPABWL scheme protects each working waveband-path by either a backup waveband-path or multiple backup lightpaths. Heuristic algorithms adopting random optimization technique are proposed for both the schemes. The performance of the two protection schemes is studied and compared. Simulation results show that both the heuristics can obtain optimum solutions and the MPABWL scheme leads to less switching and transmission costs than the PBABL scheme.

Same-Destination-Intermediate Grouping vs. End-to-End Grouping for Waveband Switching in WDM Mesh Networks

We investigate waveband switching (WBS) with different grouping strategies in wavelength-division multiplexing (WDM) mesh networks. End-to-end waveband switching (ETEWBS) and same-destination-intermediate waveband switching (SD-IT-WBS) are analyzed and compared in terms of blocking probability and cost savings. First, an analytical model for ETEWBS is proposed to determine the network blocking probability in a mesh network. For SD-IT-WBS, a simple waveband switching algorithm is presented. An analytical model to determine the network blocking probability is proposed for SD-IT-WBS based on the algorithm. The analytical results are validated by comparing with simulation results. Both results match well and show that ETE-WBS slightly outperforms SD-IT-WBS in terms of blocking probability. On the other hand, simulation results show that SD-IT-WBS outperforms ETE-WBS in terms of cost savings.

A Novel Cost-Efficient On-Line Intermediate Waveband-Switching Scheme in WDM Mesh Networks

Waveband switching (WBS) is an important technique to save switching and transmission cost in wavelength -division multiplexed (WDM) optical networks. A cost-efficient WBS scheme would enable network carriers to increase the network throughput (revenue) while achieving significant cost savings. We identify the critical factors that determine the WBS network throughput and switching cost and propose a novel intermediate waveband switching (IT-WBS) algorithm, called the minimizing-weighted-cost (MWC) algorithm. The MWC algorithm defines a cost for each candidate route of a call. By selecting the route with the smallest weighted cost, MWC balances between minimizing the call blocking probability and minimizing the network switching cost. Our simulations show that MWC outperforms other wavelength/waveband switching algorithms and can enhance the network throughput at a reduced cost.

Discontinuous Waveband Switching in WDM Optical Networks

Routing techniques used in wavelength routed optical networks (WRN) do not give an efficient solution with Waveband routed optical networks (WBN) as the objective of routing in WRN is to reduce the blocking probability and that in WBN is to reduce the number of switching ports. Routing in WBN can be divided two parts, finding the route and grouping the wavelength assigned into that route with some existing wavelengths/wavebands. In this paper, we propose a heuristic for waveband routing, which uses a new grouping strategy called discontinuous waveband grouping to group the wavelengths into a waveband. The main objective of our algorithm is to decrease the total number of ports required and reduce the blocking probability of the network. The performance of the heuristic is analyzed using simulation on a WBN with non-uniform wavebands.

A Generic Autonomous Clustering-Based Heterogeneous Waveband Switching Architecture in WDM Networks

Heterogeneous waveband switching (HeteroWBS) in WDM networks reduces the network operational costs. We propose an autonomous clustering-based HeteroWBS architecture to support the design of efficient HeteroWBS algorithms under dynamic traffic requests in such a network.

A Graph Model for DynamicWaveband Switching in WDM Mesh Networks

We investigate the problem of waveband switching (WBS) in a wavelength-division multiplexing (WDM) mesh network with dynamic traffic requests. To solve the WBS problem in a homogeneous dynamic WBS network, where every node is a multi-granular optical cross-connect (MG-OXC), we construct an auxiliary graph. Based on the auxiliary graph, we develop two heuristic on-line WBS algorithms with different grouping policies, namely the wavelength-first WBS algorithm based on the auxiliary graph (WFAUG) and the waveband-first WBS algorithm based on the auxiliary graph (BFAUG). Our results show that the WFAUG algorithm outperforms the BFAUG algorithm.

Integrated Intermediate Waveband and Wavelength Switching for Optical WDM Mesh Networks

As wavelength-division multiplexing (WDM) evolves towards practical applications in optical transport networks, waveband switching (WBS) has been introduced to cut down the operational costs and to reduce the complexities and sizes of network components, e.g., optical cross-connects (OXCs). This paper considers the routing, wavelength assignment and waveband assignment (RWWBA) problem in a WDM network supporting mixed waveband and wavelength switching. First, the techniques supporting waveband switching are studied, where a node architecture enabling mixed waveband and wavelength switching is proposed. Second, to solve the RWWBA problem with reduced switching costs and improved network throughput, the cost savings and call blocking probabilities along intermediate waveband-routes are analyzed. Our analysis reveals some important insights about the cost savings and call blocking probability in relation to the fiber capacity, the candidate path, and the traffic load. Third, based on our analysis, an online integrated intermediate WBS algorithm (IIWBS) is proposed. IIWBS determines the waveband switching route for a call along its candidate path according to the node connectivity, the link utilization, and the path length information. In addition, the IIWBS algorithm is adaptive to real network applications under dynamic traffic requests. Finally, our simulation results show that IIWBS outperforms a previous intermediate WBS algorithm and RWA algorithms in terms of network throughput and cost efficiency.

The Raccoon, a New Host for Microphallus sp., with Additional Notes on M. ovatus from Turtles

Discusses the raccoon, a new host for Microphallus sp., with additional notes on M. ovatus from turtles.

Killer Whales and Marine Mammal Trends in The North Pacific—A Re-Examination of Evidence for Sequential Megafauna Collapse and the Prey-Switching Hypothesis

Springer et al. (2003) contend that sequential declines occurred in North Pacific populations of harbor and fur seals, Steller sea lions, and sea otters. They hypothesize that these were due to increased predation by killer whales, when industrial whaling’s removal of large whales as a supposed primary food source precipitated a prey switch. Using a regional approach, we reexamined whale catch data, killer whale predation observations, and the current biomass and trends of potential prey, and found little support for the prey-switching hypothesis. Large whale biomass in the Bering Sea did not decline as much as suggested by Springer et al., and much of the reduction occurred 50–100 yr ago, well before the declines of pinnipeds and sea otters began; thus, the need to switch prey starting in the 1970s is doubtful. With the sole exception that the sea otter decline followed the decline of pinnipeds, the reported declines were not in fact sequential. Given this, it is unlikely that a sequential megafaunal collapse from whales to sea otters occurred. The spatial and temporal patterns of pinniped and sea otter population trends are more complex than Springer et al. suggest, and are often inconsistent with their hypothesis. Populations remained stable or increased in many areas, despite extensive historical whaling and high killer whale abundance. Furthermore, observed killer whale predation has largely involved pinnipeds and small cetaceans; there is little evidence that large whales were ever a major prey item in high latitudes. Small cetaceans (ignored by Springer et al.) were likely abundant throughout the period. Overall, we suggest that the Springer et al. hypothesis represents a misleading and simplistic view of events and trophic relationships within this complex marine ecosystem.

A Novel Fiber Delay Line Buffering Architecture for Optical Packet Switching

Due to the lack of optical random access memory, optical fiber delay line (FDL) is currently the only way to implement optical buffering. Feed-forward and feedback are two kinds of FDL structures in optical buffering. Both have advantages and disadvantages. In this paper, we propose a more effective hybrid FDL architecture that combines the merits of both schemes. The core of this switch is the arrayed waveguide grating (AWG) and the tunable wavelength converter (TWC). It requires smaller optical device sizes and fewer wavelengths and has less noise than feedback architecture. At the same time, it can facilitate preemptive priority routing which feed-forward architecture cannot support. Our numerical results show that the new switch architecture significantly reduces packet loss probability.

On the Ecology and Distribution of Echinococcus spp. (Cestoda: Taeniidae), and Characteristics of Their Development in the Intermediate Host. II. Comparative Studies on the Development of Larval E. multilocularis Leuckart, 1863, in the Intermediate Host

This paper reports the results of a comparative study of the development of the larval Echinococcus multilocularis Leuckart, 1863), and associated tissue reaction in naturally and experimentally infected mammals representing 31 species. The histogenesis of the larval cestode was traced in detail in arvicoline rodents of several species, and interspecific differences were defined. In arvicoline rodents, the developing larva exhibited host-specific characteristics within about a month after infection was established. The tissue reaction in Microtus oeconomus was characterized by the production of a large quantity of detritus around the larva, and by the formation of a thick epithelioid zone. In one subspecies, M. oeconomus innuitus, development of the larva was retarded, and the detrital mass was often calcified; in another, M. oeconomus operarius, the detritus rarely became calcified and the larva proliferated more rapidly. In M. pennsylvanicus, the tissue reaction was minimal, and little detritus was present. The characteristics of the tissue reaction in M. montebelli placed it in an intermediate position between the aforementioned species. In Clethrionomys rutilus, a thin epithelioid zone and an outer zone of loose collagenous fibers composed the adventitial layer; exogenous budding was retarded in this vole. A minimal tissue reaction occurred in Lagurus curtatus. In Lemmus spp., larger cysts were characteristic, but areas of small-cystic proliferation were always present. Similar differences in species or subspecies of Citellus and Dicrostonyx were described. Lesions of alveolar bydatid disease in man also were studied. The invasive growth of the larval cestode in the human liver involves a process comparable to small-cystic proliferation in the natural intermediate hosts. Although the later stages of development of the larval cestode are inhibited in man, exogenous proliferation of vesicles continues for the life of the host. The lesion in man was compared with a morphologically similar formation produced by anomalous development of the larval E. granulosus in the bovine liver. The latter is distinguished by the absence of areas of small-cystic proliferation. Non-echinococcal lesions found in the tissues studied, some of which resembled foci caused by the larval E. multilocularis, were briefly discussed.

Host-Specificity of Myxoma Virus: Pathogenesis of South American and North American Strains of Myxoma Virus in Two North American Lagomorph Species

The pathogenesis of South American and North American myxoma viruses was examined in two species of North American lagomorphs, Sylvilagus nuttallii (mountain cottontail) and Sylvilagus audubonii (desert cottontail) both of which have been shown to have the potential to transmit the South American type of myxoma virus. Following infection with the South American strain (Lausanne, Lu), S. nuttallii developed both a local lesion and secondary lesions on the skin. They did not develop the classical myxomatosis seen in European rabbits (Oryctolagus cuniculus). The infection at the inoculation site did not resolve during the 20-day time course of the trial and contained transmissible virus titres at all times. In contrast, S. audubonii infected with Lu had very few signs of disseminated infection and partially controlled virus replication at the inoculation site. The prototype Californian strain of myxoma virus (MSW) was able to replicate at the inoculation site of both species but did not induce clinical signs of a disseminated infection. In S. audubonii, there was a rapid response to MSW characterized by a massive T lymphocyte infiltration of the inoculation site by day 5. MSW did not reach transmissible titres at the inoculation site in either species. This might explain why the Californian myxoma virus has not expanded its host-range in North America.

INFLUENCE OF TYPE 2 BOVINE VIRAL DIARRHEA VIRUS NPRO ON ENHANCEMENT OF BOVINE RESPIRATORY SYNCYTIAL VIRUS REPLICATION MEDIATED BY ANTAGONISM OF HOST CELL INTERFERON TYPE I RESPONSES

Bovine viral diarrhea virus (BVDV) is a member of the genus Pestivirus, Family Flaviviridae. The virus can infect many species of animals of the order Artiodactyla. The BVDV genome encodes an auto protease, Npro, that degrades interferon regulatory factor-3 (IRF-3) reducing type I interferon (IFN-I) production from host cells. Bovine respiratory syncytial virus (BRSV) is a member of the genus Pneumovirus, Family Paramyxoviridae. Concurrent infection with BVDV and BRSV causes more severe respiratory and enteric disease than infection with either virus alone. Our hypothesis was that Npro modulates the innate immune responses to BVDV infection and enhances replication of BVDV or BRSV co-infection. The noncytopathic BVDV2 viruses NY93/c N- Npro 18 EGFP (a mutant with modified Npro fused with enhanced green fluorescent protein), NY93 infectious clone (NY93/c), wild-type NY93-BVDV2 (NY93-wt), and BRSV were evaluated in this study. The objectives of this study were: (1) to characterize the replication kinetics and IFN-I induction in Madin-Darby bovine kidney (MDBK) cells following infection with each of the BVDV isolates, and (2) to characterize the influence of BVDV-mediated IFN-I antagonism on enhancement of BRSV replication in bovine turbinate (BT) cells. NY93/c N- Npro 18 EGFP replicated 0.4 – 1.6 TCID50 logs lower than NY93-wt in MDBK cells. NY93/c N- Npro 18 EGFP-infected MDBK cells synthesized IFN-I significantly higher than NY93/c- and NY93-wt-infected MDBK cells. BT cells co-infected with NY93/c N- Npro 18 EGFP/BRSV or NY93-wt/BRSV were evaluated to determine the effects of co-infection on BRSV replication and IFN-I induction in BT cells. BRSV RNA levels in NY93-wt/BRSV co-infected BT cells were 2.49, 2.79, and 2.89 copy number logs significantly greater than in NY93/c N- Npro 18 EGFP/BRSV co-infected BT cells on days 5, 7, and 9 post-infection, respectively. BVDV RNA levels in NY93/c N- Npro 18 EGFP-infected BT cells were 1.64 – 4.38 copy number logs lower than in NY93-wt-infected BT cells. NY93/c N- Npro 18 EGFP single and co-infected BT cells synthesized IFN-I significantly higher than NY93-wt single and co-infected BT cells. In summary, these findings suggest: (1) NY93/c N- Npro 18 EGFP BVDV2 induced higher levels of IFN-I than BVDV2-wt and may be useful as a safer, replicating BVDV vaccine, and (2) Enhancement of BRSV infection by BVDV co-infection is mediated by antagonism of IFN-I.

Selection of Switching Sites in All-Optical Nework Topology Design

In this paper, we consider the problem of topology design for optical networks. We investigate the problem of selecting switching sites to minimize total cost of the optical network. The cost of an optical network can be expressed as a sum of three main factors: the site cost, the link cost, and the switch cost. To the best of our knowledge, this problem has not been studied in its general form as investigated in this paper. We present a mixed integer quadratic programming (MIQP) formulation of the problem to find the optimal value of the total network cost. We also present an efficient heuristic to approximate the solution in polynomial time. The experimental results show good performance of the heuristic. The value of the total network cost computed by the heuristic varies within 2% to 21% of its optimal value in the experiments with 10 nodes. The total network cost computed by the heuristic for 51% of the experiments with 10 node network topologies varies within 8% of its optimal value. We also discuss the insight gained from our experiments.