Refuge Update – November/December 2005, Volume 2, Number 6

Table of Contents: Make Way for Ducklings, page 4 With help from refuge experts, roads and bridges can be built to accommodate wildlife. Katrina Heroes, pages 8-9 Extraordinary diaries from refuge staffers who were there when Katrina came calling. Focus on…Reaching Youth , page 10-15 Refuges give young people a chance to learn art, poetry, native culture, service – and stewardship. Nisqually: Growing and Restoring, page 17 The Outstanding Refuge Plan of 2005 opens the door to the largest estuary restoration project in the Pacific Northwest.

American Lives: A Reader [Preface & Introduction]

Memoirs are as varied as human emotion and experience, and those published in the distinguished American Lives Series run the gamut. Excerpted from this series (called “splendid” by Newsweek) and collected here for the first time, these dispatches from American lives take us from China during the Cultural Revolution to the streets of New York in the sixties to a cabin in the backwoods of Idaho. In prose as diverse as the stories they tell, writers such as Floyd Skloot, Ted Kooser, Peggy Shumaker, and Lee Martin, among many others, open windows to their own ordinary and extraordinary experiences. John Skoyles tells how, for his Uncle Fred, a particular “Hard Luck Suit” imparted misfortune. Brenda Serotte describes a Turkish grandmother who made her living reading palms, interpreting cups, and prescribing poultices for the community. In “Son of Mr. Green Jeans,” Dinty W. Moore views fatherhood through the lens of pop culture. Janet Sternburg’s Phantom Limb muses on the dilemmas of a child caring for a parent. Whether evoking moments of death or disease, in family or marriage, history, politics, religion, or culture, these glimpses into singular American lives come together in a richly textured, colorful patchwork quilt of American life.

Politics and Trade in the Former Soviet Union

Fifteen independent countries emerged from the collapse of the Soviet Union in 1989-91. Aside from the Russian Federation, the former Soviet Republics lie in four geographic regions: the Caucasus (Armenia, Azerbaijan, Georgia); Central Asia (Kazakhstan, Kyrgyzstan, Tajikistan, Turkmenistan, and Uzbekistan); the Baltics (Estonia, Latvia, Lithuania); and Eastern Europe (Belarus, Moldova, Ukraine).

A macroscopic kinetic model for DNA polymerase elongation and high-fidelity nucleotide election

The enzymatically catalyzed template-directed extension of ssDNA/primer complex is an impor-tant reaction of extraordinary complexity. The DNA polymerase does not merely facilitate the insertion of dNMP, but it also performs rapid screening of substrates to ensure a high degree of fidelity. Several kinetic studies have determined rate constants and equilibrium constants for the elementary steps that make up the overall pathway. The information is used to develop a macro-scopic kinetic model, using an approach described by Ninio [Ninio J., 1987. Alternative to the steady-state method: derivation of reaction rates from first-passage times and pathway probabili-ties. Proc. Natl. Acad. Sci. U.S.A. 84, 663–667]. The principle idea of the Ninio approach is to track a single template/primer complex over time and to identify the expected behavior. The average time to insert a single nucleotide is a weighted sum of several terms, in-cluding the actual time to insert a nucleotide plus delays due to polymerase detachment from ei-ther the ternary (template-primer-polymerase) or quaternary (+nucleotide) complexes and time delays associated with the identification and ultimate rejection of an incorrect nucleotide from the binding site. The passage times of all events and their probability of occurrence are ex-pressed in terms of the rate constants of the elementary steps of the reaction pathway. The model accounts for variations in the average insertion time with different nucleotides as well as the in-fluence of G+C content of the sequence in the vicinity of the insertion site. Furthermore the model provides estimates of error frequencies. If nucleotide extension is recognized as a compe-tition between successful insertions and time delaying events, it can be described as a binomial process with a probability distribution. The distribution gives the probability to extend a primer/template complex with a certain number of base pairs and in general it maps annealed complexes into extension products.