IANR Day

We are called to great work, you and I. We are called to fulfill the mission of the land-grant university, and I consider that as much a distinct honor as it is a serious responsibility. I am so pleased to be able to meet that honor and that responsibility by working with all of you, and I’m delighted to talk with you today. While I haven’t had the opportunity to meet everyone in this room, I’ve certainly been glad for all the opportunities that have arisen to meet some of you. And I look forward to getting to know others. I am excited to be here in Nebraska, and I’m enthusiastic about all I think we can do, working together, to advance the mission of the Instituted and the University, and to be of service to the citizens of Nebraska.

Plattsmouth Rotary February Meeting (Farmer's Day Program)

Good afternoon. It's a pleasure to be with you here in Plattsmouth today, and I am particularly pleased to be part of your Farmer's Day program. Because I am so new to Nebraska and the University of Nebraska Institute of Agriculture and Natural Resources, I am trying to learn as much as I can about how the Institute connects with the state, annd how you feel we can be an even better partner with Nebraska. I'm curious about our work in each Nebraska county, and because I was coming to Cass County today, I asked those I work with for some figures on how the Institute is part of the lives of Cass Countians.

UAAD Founders' Day

Because today is the birthday of Thomas Edison who was born in 1847 in Ohio, I suppose that you would expect me as today's speaker to have a special obligation to be inventive. Some of you probably know that for several years, Edison's birthday has been observed as National Science Youth Day, which sounds to me like a rather interesting invention itself. Youth is not a science and science is certainly not a youth, but I am perfectly willing to go along with the national celebration and wish a happy birthday to any science youth that I might happen to encounter. I would encourage you to do the same.

Panhandle Research and Extension Center Field Day

Commodity trader. Financial analyst. Human resources director. Marketing analyst. Lending officer. Stockbroker. Public relations specialist. Zookeeper. Nutritionist. These are only a few of the varied careers recent College of Agricultural Sciences and Natural Resources graduates are pursuing these days. Exciting, fulfilling careers for which the College of Agricultural Sciences and Natural Resources - we call the college CASNR for short - provides a tremendous educational base that students can build on for their lives.

2006 East Campus Arbor Day Celebration

It cannot be lost on those of us present here today that Nebraska, the land of tall skies and wide prairies, where early settlers saw grass roll to the horizon broken only by the call of the lark, is the home of Arbor Day. Nebraska is, indeed, the tree planter state.

2007 East Campus Arbor Day Celebration

As we celebrate Arbor Day, it seems fitting to begin with a J. Sterling Morton quote. "The cultivation of trees is the cultivation of the good, the beautiful, and the ennobling in man,” Morton once said, adding, "and for one, I wish to see it become universal."

KRVN - Gudmundsen Field Day

John, the Institute of Agriculture and Natural Resources has various locations throughout Nebraska for research and educational purposes. One such location is the Gudmundsen Sandhills Laboratory, which this past week was the site for the annual Youth Field Day.

A macroscopic kinetic model for DNA polymerase elongation and high-fidelity nucleotide election

The enzymatically catalyzed template-directed extension of ssDNA/primer complex is an impor-tant reaction of extraordinary complexity. The DNA polymerase does not merely facilitate the insertion of dNMP, but it also performs rapid screening of substrates to ensure a high degree of fidelity. Several kinetic studies have determined rate constants and equilibrium constants for the elementary steps that make up the overall pathway. The information is used to develop a macro-scopic kinetic model, using an approach described by Ninio [Ninio J., 1987. Alternative to the steady-state method: derivation of reaction rates from first-passage times and pathway probabili-ties. Proc. Natl. Acad. Sci. U.S.A. 84, 663–667]. The principle idea of the Ninio approach is to track a single template/primer complex over time and to identify the expected behavior. The average time to insert a single nucleotide is a weighted sum of several terms, in-cluding the actual time to insert a nucleotide plus delays due to polymerase detachment from ei-ther the ternary (template-primer-polymerase) or quaternary (+nucleotide) complexes and time delays associated with the identification and ultimate rejection of an incorrect nucleotide from the binding site. The passage times of all events and their probability of occurrence are ex-pressed in terms of the rate constants of the elementary steps of the reaction pathway. The model accounts for variations in the average insertion time with different nucleotides as well as the in-fluence of G+C content of the sequence in the vicinity of the insertion site. Furthermore the model provides estimates of error frequencies. If nucleotide extension is recognized as a compe-tition between successful insertions and time delaying events, it can be described as a binomial process with a probability distribution. The distribution gives the probability to extend a primer/template complex with a certain number of base pairs and in general it maps annealed complexes into extension products.