1 resultado para in-silico
em Digital Commons @ DU | University of Denver Research
Filtro por publicador
- Aberdeen University (1)
- Abertay Research Collections - Abertay University’s repository (1)
- Aberystwyth University Repository - Reino Unido (3)
- Academic Archive On-line (Stockholm University; Sweden) (1)
- Acceda, el repositorio institucional de la Universidad de Las Palmas de Gran Canaria. España (2)
- Adam Mickiewicz University Repository (3)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (19)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (4)
- ARCA - Repositório Institucional da FIOCRUZ (3)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (17)
- Archimer: Archive de l'Institut francais de recherche pour l'exploitation de la mer (2)
- Archive of European Integration (3)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (6)
- Aston University Research Archive (43)
- Biblioteca de Teses e Dissertações da USP (5)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (28)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (8)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (5)
- Bioline International (3)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (52)
- Boston University Digital Common (1)
- Brock University, Canada (1)
- CaltechTHESIS (2)
- Cambridge University Engineering Department Publications Database (3)
- CentAUR: Central Archive University of Reading - UK (19)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (9)
- CORA - Cork Open Research Archive - University College Cork - Ireland (8)
- Deakin Research Online - Australia (16)
- Department of Computer Science E-Repository - King's College London, Strand, London (5)
- Digital Commons - Michigan Tech (1)
- Digital Commons @ DU | University of Denver Research (1)
- Digital Commons at Florida International University (4)
- DigitalCommons@The Texas Medical Center (10)
- Doria (National Library of Finland DSpace Services) - National Library of Finland, Finland (1)
- Duke University (6)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (7)
- FAUBA DIGITAL: Repositorio institucional científico y académico de la Facultad de Agronomia de la Universidad de Buenos Aires (2)
- FUNDAJ - Fundação Joaquim Nabuco (1)
- Glasgow Theses Service (2)
- Greenwich Academic Literature Archive - UK (1)
- Helda - Digital Repository of University of Helsinki (9)
- Illinois Digital Environment for Access to Learning and Scholarship Repository (1)
- Indian Institute of Science - Bangalore - Índia (22)
- Institutional Repository of Leibniz University Hannover (3)
- Instituto Gulbenkian de Ciência (1)
- Instituto Nacional de Saúde de Portugal (3)
- Lume - Repositório Digital da Universidade Federal do Rio Grande do Sul (1)
- National Center for Biotechnology Information - NCBI (9)
- Nottingham eTheses (2)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (1)
- Publishing Network for Geoscientific & Environmental Data (1)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (50)
- Queensland University of Technology - ePrints Archive (197)
- RCAAP - Repositório Científico de Acesso Aberto de Portugal (2)
- RDBU - Repositório Digital da Biblioteca da Unisinos (1)
- ReCiL - Repositório Científico Lusófona - Grupo Lusófona, Portugal (1)
- Repositorio Academico Digital UANL (1)
- Repositório Alice (Acesso Livre à Informação Científica da Embrapa / Repository Open Access to Scientific Information from Embrapa) (3)
- Repositorio de la Universidad de Cuenca (1)
- Repositório Digital da UNIVERSIDADE DA MADEIRA - Portugal (1)
- Repositorio Institucional da UFLA (RIUFLA) (1)
- Repositório Institucional da Universidade de Aveiro - Portugal (4)
- Repositório Institucional da Universidade de Brasília (2)
- Repositório Institucional da Universidade Estadual de São Paulo - UNESP (2)
- Repositório Institucional da Universidade Federal do Rio Grande - FURG (1)
- Repositorio Institucional de la Universidad de Málaga (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (87)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (1)
- SAPIENTIA - Universidade do Algarve - Portugal (12)
- Savoirs UdeS : plateforme de diffusion de la production intellectuelle de l’Université de Sherbrooke - Canada (2)
- School of Medicine, Washington University, United States (1)
- Universidad de Alicante (1)
- Universidad del Rosario, Colombia (7)
- Universidad Politécnica de Madrid (11)
- Universidade Complutense de Madrid (1)
- Universidade de Lisboa - Repositório Aberto (3)
- Universidade Federal de Uberlândia (1)
- Universidade Federal do Pará (2)
- Universidade Federal do Rio Grande do Norte (UFRN) (29)
- Universita di Parma (4)
- Universitat de Girona, Spain (2)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (2)
- Université de Lausanne, Switzerland (3)
- Université de Montréal (4)
- Université de Montréal, Canada (33)
- University of Queensland eSpace - Australia (19)
- University of Southampton, United Kingdom (1)
- WestminsterResearch - UK (2)
Resumo:
Fragile X syndrome (FXS) is the most common form of inherited mental retardation in humans. FXS is caused by loss of the Fragile X Mental Retardation Protein (FMRP), an important regulator of neuronal mRNA translation. Patients with FXS display cognitive deficits including memory problems. Protein synthesis-dependent long-term changes in synaptic plasticity are involved in the establishment and maintenance of long-term memory. One prevalent theory of FXS pathology predicts that FMRP is required to negatively regulate the translation of important mRNAs at the synapse. We are investigating microRNAs (miRNAs) as a potential regulator of synaptic FMRP-regulated mRNAs that have previously been described as being crucial to the process of synaptic plasticity. The general hypothesis underlying this thesis is that FMRP may negatively regulate the expression of futsch (the Drosophila homologue of the microtubule-associated protein gene MAP1B) via the miRNA pathway. The first step we took in testing this hypothesis was to confirm that futsch is subject to miRNA-mediated translational control. Using in silico target analysis, we predicted that several neuronally expressed miRNAs target the futsch mRNA 3'UTR and repress expression of Futsch protein. Then, using an in vitro luciferase reporter system, we showed that miR-315 and members of the miR-9 family selectively down-regulated futsch reporter translation. We have confirmed by site- directed mutagenesis that the miRNA interaction with the futsch 3'UTR is specific to the miRNA seed region binding site. Interestingly, reduction of FMRP levels by RNAi had no effect on futsch 3'UTR reporter expression. Together, these data suggest regulation of futsch expression by the miRNA pathway might be independent of FMRP activity. However, additional experiments need to be completed to confirm these preliminary results.