1 resultado para FREE CASH FLOW
em DI-fusion - The institutional repository of Université Libre de Bruxelles
Filtro por publicador
- KUPS-Datenbank - Universität zu Köln - Kölner UniversitätsPublikationsServer (1)
- Academic Research Repository at Institute of Developing Economies (1)
- Acceda, el repositorio institucional de la Universidad de Las Palmas de Gran Canaria. España (1)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (4)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (2)
- Aquatic Commons (4)
- Archive of European Integration (2)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (3)
- Aston University Research Archive (17)
- Biblioteca de Teses e Dissertações da USP (2)
- Biblioteca Digital - Universidad Icesi - Colombia (1)
- Biblioteca Digital | Sistema Integrado de Documentación | UNCuyo - UNCUYO. UNIVERSIDAD NACIONAL DE CUYO. (2)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (10)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (11)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (1)
- Bioline International (1)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (8)
- Brock University, Canada (1)
- Bucknell University Digital Commons - Pensilvania - USA (2)
- CaltechTHESIS (5)
- Cambridge University Engineering Department Publications Database (18)
- CentAUR: Central Archive University of Reading - UK (25)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (38)
- CiencIPCA - Instituto Politécnico do Cávado e do Ave, Portugal (1)
- Cochin University of Science & Technology (CUSAT), India (1)
- Coffee Science - Universidade Federal de Lavras (1)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (2)
- Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest (11)
- Deakin Research Online - Australia (29)
- DI-fusion - The institutional repository of Université Libre de Bruxelles (1)
- Digital Commons - Michigan Tech (1)
- Digital Commons at Florida International University (6)
- Digital Peer Publishing (1)
- Digital Repository at Iowa State University (2)
- DigitalCommons - The University of Maine Research (1)
- DigitalCommons@The Texas Medical Center (1)
- DigitalCommons@University of Nebraska - Lincoln (4)
- DRUM (Digital Repository at the University of Maryland) (3)
- Duke University (6)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (4)
- Glasgow Theses Service (2)
- Greenwich Academic Literature Archive - UK (17)
- Helda - Digital Repository of University of Helsinki (6)
- Indian Institute of Science - Bangalore - Índia (77)
- Instituto Politécnico de Leiria (2)
- Instituto Politécnico de Viseu (4)
- Instituto Politécnico do Porto, Portugal (8)
- Lume - Repositório Digital da Universidade Federal do Rio Grande do Sul (1)
- Massachusetts Institute of Technology (2)
- National Center for Biotechnology Information - NCBI (4)
- Nottingham eTheses (1)
- Publishing Network for Geoscientific & Environmental Data (15)
- QSpace: Queen's University - Canada (1)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (19)
- Queensland University of Technology - ePrints Archive (341)
- RCAAP - Repositório Científico de Acesso Aberto de Portugal (1)
- Repositório Científico da Universidade de Évora - Portugal (1)
- Repositório Científico do Instituto Politécnico de Lisboa - Portugal (5)
- Repositório Científico do Instituto Politécnico de Santarém - Portugal (1)
- Repositório digital da Fundação Getúlio Vargas - FGV (35)
- Repositório Digital da UNIVERSIDADE DA MADEIRA - Portugal (2)
- Repositório Institucional da Universidade de Aveiro - Portugal (1)
- Repositório Institucional da Universidade Estadual de São Paulo - UNESP (2)
- Repositorio Institucional de la Universidad de Málaga (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (40)
- Repositorio Institucional Universidad de Medellín (1)
- Repositorio Institucional Universidad EAFIT - Medelin - Colombia (9)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (3)
- SAPIENTIA - Universidade do Algarve - Portugal (1)
- Savoirs UdeS : plateforme de diffusion de la production intellectuelle de l’Université de Sherbrooke - Canada (1)
- The Scholarly Commons | School of Hotel Administration; Cornell University Research (2)
- Universidad del Rosario, Colombia (20)
- Universidad Politécnica de Madrid (14)
- Universidade Complutense de Madrid (2)
- Universidade Federal do Pará (3)
- Universidade Federal do Rio Grande do Norte (UFRN) (2)
- Universidade Metodista de São Paulo (5)
- Universidade Técnica de Lisboa (3)
- Universita di Parma (1)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (1)
- Université de Lausanne, Switzerland (2)
- Université de Montréal, Canada (2)
- University of Michigan (5)
- University of Queensland eSpace - Australia (10)
Resumo:
Intracellular cytokine staining combined with flow cytometry is one of a number of assays designed to assess T-cell immune responses. It has the specific advantage of enabling the simultaneous assessment of multiple phenotypic, differentiation and functional parameters pertaining to responding T-cells, most notably, the expression of multiple effector cytokines. These attributes make the technique particularly suitable for the assessment of T-cell immune responses induced by novel tuberculosis vaccines in clinical trials. However, depending upon the particular nature of a given vaccine and trial setting, there are approaches that may be taken at different stages of the assay that are more suitable than other alternatives. In this paper, the Tuberculosis Vaccine Initiative (TBVI) TB Biomarker Working group reports on efforts to assess the conditions that will determine when particular assay approaches should be employed. We have found that choices relating to the use of fresh whole blood or peripheral blood mononuclear cells (PBMC) and frozen PBMC; use of serum-containing or serum-free medium; length of stimulation period and use of co-stimulatory antibodies can all affect the sensitivity of intracellular cytokine assays. In the case of sample material, frozen PBMC, despite some loss of sensitivity, may be more advantageous for batch analysis. We also recommend that for multi-site studies, common antibody panels, gating strategies and analysis approaches should be employed for better comparability.