The extradomain a of fibronectin enhances the efficacy of lipopolysaccharide defective Salmonella bacterins as vaccines in mice

The Extradomain A from fibronectin (EDA) has an immunomodulatory role as fusion protein with viral and tumor antigens, but its effect when administered with bacteria has not been assessed. Here, we investigated the adjuvant effect of EDA in mice immunizations against Salmonella enterica subspecies enterica serovar Enteritidis (Salmonella Enteritidis). Since lipopolysaccharide (LPS) is a major virulence factor and the LPS O-polysaccharide (O-PS) is the immunodominant antigen in serological diagnostic tests, Salmonella mutants lacking O-PS (rough mutants) represent an interesting approach for developing new vaccines and diagnostic tests to differentiate infected and vaccinated animals (DIVA tests). Here, antigenic preparations (hot-saline extracts and formalin-inactivated bacterins) from two Salmonella Enteritidis rough mutants, carrying either intact (SE Delta waaL) or deep-defective (SE Delta gal) LPS-Core, were used in combination with EDA. Biotinylated bacterins, in particular SE Delta waaL bacterin, decorated with EDAvidin (EDA and streptavidin fusion protein) improved the protection conferred by hot-saline or bacterins alone and prevented significantly the virulent infection at least to the levels of live attenuated rough mutants. These findings demonstrate the adjuvant effect of EDAvidin when administered with biotinylated bacterins from Salmonella Enteritidis lacking O-PS and the usefulness of BEDA-SE Delta waaL as non-live vaccine in the mouse model.

Influencia de patrones culturales en la alimentación durante la adolescencia

Los hábitos alimentarios de la juventud van cambiando y desarrollándose a lo largo del tiempo a causa de los patrones culturales y sociales que se establecen en el contexto. La adquisición de unos buenos hábitos es muy importante debido a que determina en gran medida nuestra salud y puede evitar la aparición de múltiples problemas sanitarios, entre los que encontramos la obesidad. Esta investigación trata de identificar, de acuerdo a la revisión bibliográfica, los factores que condicionan y definen el patrón alimentario de los y las jóvenes. En consecuencia, se han realizado cuestionarios a jóvenes de 15 y 16 años de Pamplona, durante un período de dos meses, con el fin de recabar información sobre las tendencias alimentarias en la muestra seleccionada para su posterior análisis.

The impact of investor sentiment on stock returns in emerging markets. The case of Central European markets

This is an accepted manuscript of an article published by Taylor & Francis in Eastern European Economics on July 2015, available online: http://dx.doi.org/10.1080/00128775.2015.1079139