1 resultado para Pointers of performance
em ABACUS. Repositorio de Producción Científica - Universidad Europea
Filtro por publicador
- Repository Napier (2)
- ABACUS. Repositorio de Producción Científica - Universidad Europea (1)
- Aberystwyth University Repository - Reino Unido (5)
- Academic Research Repository at Institute of Developing Economies (1)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (8)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (1)
- Aquatic Commons (1)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (2)
- Archimer: Archive de l'Institut francais de recherche pour l'exploitation de la mer (1)
- Archive of European Integration (2)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (3)
- Aston University Research Archive (78)
- Biblioteca de Teses e Dissertações da USP (1)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (7)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (1)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (3)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (34)
- Boston University Digital Common (2)
- Brock University, Canada (12)
- Bucknell University Digital Commons - Pensilvania - USA (3)
- Bulgarian Digital Mathematics Library at IMI-BAS (1)
- Cambridge University Engineering Department Publications Database (22)
- CentAUR: Central Archive University of Reading - UK (37)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (7)
- CiencIPCA - Instituto Politécnico do Cávado e do Ave, Portugal (2)
- Cochin University of Science & Technology (CUSAT), India (3)
- Coffee Science - Universidade Federal de Lavras (2)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (1)
- CORA - Cork Open Research Archive - University College Cork - Ireland (7)
- Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest (5)
- Dalarna University College Electronic Archive (9)
- Deakin Research Online - Australia (117)
- Department of Computer Science E-Repository - King's College London, Strand, London (2)
- DI-fusion - The institutional repository of Université Libre de Bruxelles (1)
- Digital Archives@Colby (2)
- Digital Commons - Michigan Tech (4)
- Digital Commons @ DU | University of Denver Research (2)
- Digital Commons at Florida International University (30)
- Digital Peer Publishing (3)
- DigitalCommons@The Texas Medical Center (5)
- Doria (National Library of Finland DSpace Services) - National Library of Finland, Finland (1)
- DRUM (Digital Repository at the University of Maryland) (6)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (5)
- Glasgow Theses Service (3)
- Greenwich Academic Literature Archive - UK (4)
- Helda - Digital Repository of University of Helsinki (8)
- Illinois Digital Environment for Access to Learning and Scholarship Repository (1)
- Indian Institute of Science - Bangalore - Índia (31)
- Institute of Public Health in Ireland, Ireland (1)
- Instituto Politécnico de Viseu (1)
- Instituto Politécnico do Porto, Portugal (1)
- Instituto Superior de Psicologia Aplicada - Lisboa (2)
- Iowa Publications Online (IPO) - State Library, State of Iowa (Iowa), United States (1)
- Memoria Académica - FaHCE, UNLP - Argentina (3)
- Memorial University Research Repository (1)
- National Center for Biotechnology Information - NCBI (2)
- Nottingham eTheses (3)
- Portal de Revistas Científicas Complutenses - Espanha (2)
- Projetos e Dissertações em Sistemas de Informação e Gestão do Conhecimento (1)
- QSpace: Queen's University - Canada (3)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (60)
- Queensland University of Technology - ePrints Archive (148)
- RCAAP - Repositório Científico de Acesso Aberto de Portugal (1)
- Repositório Alice (Acesso Livre à Informação Científica da Embrapa / Repository Open Access to Scientific Information from Embrapa) (1)
- Repositório Científico do Instituto Politécnico de Lisboa - Portugal (2)
- Repositório digital da Fundação Getúlio Vargas - FGV (15)
- Repositório Institucional da Universidade de Aveiro - Portugal (6)
- Repositório Institucional da Universidade Federal do Rio Grande do Norte (2)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (36)
- Repositorio Institucional UNISALLE - Colombia (1)
- Research Open Access Repository of the University of East London. (1)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (3)
- School of Medicine, Washington University, United States (1)
- Scielo Uruguai (1)
- The Scholarly Commons | School of Hotel Administration; Cornell University Research (1)
- Universidad de Alicante (2)
- Universidad del Rosario, Colombia (1)
- Universidad Politécnica de Madrid (23)
- Universidade Complutense de Madrid (2)
- Universidade Federal do Pará (1)
- Universidade Federal do Rio Grande do Norte (UFRN) (8)
- Universidade Técnica de Lisboa (1)
- Universitat de Girona, Spain (2)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (1)
- Université de Montréal (3)
- Université de Montréal, Canada (15)
- Université Laval Mémoires et thèses électroniques (1)
- University of Canberra Research Repository - Australia (2)
- University of Connecticut - USA (1)
- University of Michigan (13)
- University of Queensland eSpace - Australia (32)
- University of Washington (4)
- WestminsterResearch - UK (2)
- Worcester Research and Publications - Worcester Research and Publications - UK (9)
Resumo:
High quality, pure DNA is required for ensuring reliable and reproducible results in molecular diagnosis applications. A number of in-house and commercial methods are available for the extraction and purification of genomic DNA from faecal material, each one offering a specific combination of performance, cost-effectiveness, and easiness of use that should be conveniently evaluated in function of the pathogen of interest. In this comparative study the marketed kits QIAamp DNA stool mini (Qiagen), SpeedTools DNA extraction (Biotools), DNAExtract-VK (Vacunek), PowerFecal DNA isolation (MoBio), and Wizard magnetic DNA purification system (Promega Corporation) were assessed for their efficacy in obtaining DNA of the most relevant enteric protozoan parasites associated to gastrointestinal disease globally. A panel of 113 stool specimens of clinically confirmed patients with cryptosporidiosis (n = 29), giardiasis (n = 47) and amoebiasis by Entamoeba histolytica (n = 3) or E. dispar (n = 10) and apparently healthy subjects (n = 24) were used for this purpose. Stool samples were aliquoted in five sub-samples and individually processed by each extraction method evaluated. Purified DNA samples were subsequently tested in PCR-based assays routinely used in our laboratory. The five compared methods yielded amplifiable amounts of DNA of the pathogens tested, although performance differences were observed among them depending on the parasite and the infection burden. Methods combining chemical, enzymatic and/or mechanical lysis procedures at temperatures of at least 56 °C were proven more efficient for the release of DNA from Cryptosporidium oocysts.