1 resultado para 1074
em ABACUS. Repositorio de Producción Científica - Universidad Europea
Filtro por publicador
- Rhode Island School of Design (1)
- University of Cagliari UniCA Eprints (1)
- ABACUS. Repositorio de Producción Científica - Universidad Europea (1)
- Aberystwyth University Repository - Reino Unido (1)
- Acceda, el repositorio institucional de la Universidad de Las Palmas de Gran Canaria. España (1)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (2)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (1)
- Aquatic Commons (12)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (1)
- Archive of European Integration (2)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (3)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (23)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (31)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (150)
- Boston College Law School, Boston College (BC), United States (1)
- Boston University Digital Common (1)
- Bucknell University Digital Commons - Pensilvania - USA (3)
- Cámara de Comercio de Bogotá, Colombia (1)
- Cambridge University Engineering Department Publications Database (10)
- CentAUR: Central Archive University of Reading - UK (74)
- Chapman University Digital Commons - CA - USA (2)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (37)
- Cochin University of Science & Technology (CUSAT), India (2)
- Collection Of Biostatistics Research Archive (2)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (7)
- CUNY Academic Works (2)
- Dalarna University College Electronic Archive (1)
- Deakin Research Online - Australia (46)
- Department of Computer Science E-Repository - King's College London, Strand, London (1)
- DI-fusion - The institutional repository of Université Libre de Bruxelles (2)
- Digital Archives@Colby (3)
- Digital Commons - Michigan Tech (1)
- Digital Commons - Montana Tech (2)
- Digital Commons @ Winthrop University (1)
- Digital Howard @ Howard University | Howard University Research (1)
- Digital Repository at Iowa State University (1)
- DigitalCommons - The University of Maine Research (2)
- DigitalCommons@The Texas Medical Center (5)
- DigitalCommons@University of Nebraska - Lincoln (7)
- Digitale Sammlungen - Goethe-Universität Frankfurt am Main (3)
- Diposit Digital de la UB - Universidade de Barcelona (1)
- Duke University (5)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (3)
- Gallica, Bibliotheque Numerique - Bibliothèque nationale de France (French National Library) (BnF), France (8)
- Greenwich Academic Literature Archive - UK (11)
- Helda - Digital Repository of University of Helsinki (2)
- Indian Institute of Science - Bangalore - Índia (48)
- Instituto Politécnico do Porto, Portugal (1)
- Livre Saber - Repositório Digital de Materiais Didáticos - SEaD-UFSCar (1)
- Lume - Repositório Digital da Universidade Federal do Rio Grande do Sul (1)
- Massachusetts Institute of Technology (1)
- Memoria Académica - FaHCE, UNLP - Argentina (6)
- Ministerio de Cultura, Spain (17)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (2)
- Portal de Revistas Científicas Complutenses - Espanha (1)
- Publishing Network for Geoscientific & Environmental Data (148)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (122)
- Queensland University of Technology - ePrints Archive (53)
- Repositório Digital da UNIVERSIDADE DA MADEIRA - Portugal (2)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (61)
- SAPIENTIA - Universidade do Algarve - Portugal (1)
- School of Medicine, Washington University, United States (3)
- Universidad del Rosario, Colombia (7)
- Universidade Federal do Pará (17)
- Universidade Federal do Rio Grande do Norte (UFRN) (4)
- Universitat de Girona, Spain (2)
- Université de Lausanne, Switzerland (7)
- Université de Montréal, Canada (3)
- University of Connecticut - USA (9)
- University of Southampton, United Kingdom (1)
- WestminsterResearch - UK (3)
Resumo:
We examined the effects of cofactors and DNA on the stability, oligomeric state and conformation of the human mitochondrial DNA helicase. We demonstrate that low salt conditions result in protein aggregation that may cause dissociation of oligomeric structure. The low salt sensitivity of the mitochondrial DNA helicase is mitigated by the presence of magnesium, nucleotide, and increased temperature. Electron microscopic and glutaraldehyde cross-linking analyses provide the first evidence of a heptameric oligomer and its interconversion from a hexameric form. Limited proteolysis by trypsin shows that binding of nucleoside triphosphate produces a conformational change that is distinct from the conformation observed in the presence of nucleoside diphosphate. We find that single-stranded DNA binding occurs in the absence of cofactors and renders the mitochondrial DNA helicase more susceptible to proteolytic digestion. Our studies indicate that the human mitochondrial DNA helicase shares basic properties with the SF4 replicative helicases, but also identify common features with helicases outside the superfamily, including dynamic conformations similar to other AAA+ ATPases.