Análise de vizinhança: uma nova abordagem para avaliar a rede proposicional de mapas conceituais*

A análise detalhada de Mapas Conceituais (MCs) pode revelar informações latentes que não são percebidas a partir da mera leitura do seu conjunto de proposições. O presente trabalho tem como objetivo propor a Análise de Vizinhança (AViz) como uma forma inovadora de avaliar os MCs obtidos em sala de aula. A seleção de um Conceito Obrigatório (CO) permite ao professor verificar como os alunos o relacionam com outros conceitos, os quais são classificados como Conceitos Vizinhos (CVs). As proposições estabelecidas entre o CO e os CVs são suficientes para indicar o nível de compreensão do aluno sobre o tema mapeado. MCs (n = 69) sobre mudanças climáticas formam o primeiro conjunto de dados empíricos que ratifica o potencial da AViz. O CO selecionado foi dispersão, a fim de avaliar se os alunos conseguem relacionar esse fenômeno físico com o caráter global desse problema ambiental. Os padrães identificados a partir da AViz sugerem que, apesar de serem submetidos a uma mesma sequência didática, nem todos os alunos conseguiram utilizar o CO de forma adequada. Isso pode ser explicado a partir da Teoria da Aprendizagem Significativa de David Ausubel, que destaca o papel fundamental dos conhecimentos prévios no processo de assimilação de novas informações.

Androgen responsive intronic non-coding RNAs

Abstract Background Transcription of large numbers of non-coding RNAs originating from intronic regions of human genes has been recently reported, but mechanisms governing their biosynthesis and biological functions are largely unknown. In this work, we evaluated the existence of a common mechanism of transcription regulation shared by protein-coding mRNAs and intronic RNAs by measuring the effect of androgen on the transcriptional profile of a prostate cancer cell line. Results Using a custom-built cDNA microarray enriched in intronic transcribed sequences, we found 39 intronic non-coding RNAs for which levels were significantly regulated by androgen exposure. Orientation-specific reverse transcription-PCR indicated that 10 of the 13 were transcribed in the antisense direction. These transcripts are long (0.5–5 kb), unspliced and apparently do not code for proteins. Interestingly, we found that the relative levels of androgen-regulated intronic transcripts could be correlated with the levels of the corresponding protein-coding gene (asGAS6 and asDNAJC3) or with the alternative usage of exons (asKDELR2 and asITGA6) in the corresponding protein-coding transcripts. Binding of the androgen receptor to a putative regulatory region upstream from asMYO5A, an androgen-regulated antisense intronic transcript, was confirmed by chromatin immunoprecipitation. Conclusion Altogether, these results indicate that at least a fraction of naturally transcribed intronic non-coding RNAs may be regulated by common physiological signals such as hormones, and further corroborate the notion that the intronic complement of the transcriptome play functional roles in the human gene-expression program.

Relationship between Amazon biomass burning aerosols and rainfall over La Plata Basin

High aerosol loads are discharged into the atmosphere by biomass burning in Amazon and Central Brazil during the dry season. These particles can interact with clouds as cloud condensation nuclei (CCN) changing cloud microphysics and radiative properties and, thereby, affecting the radiative budget of the region. Furthermore, the biomass burning aerosols can be transported by the low level jet (LLJ) to La Plata Basin where many mesoscale convective systems (MCS) are observed during spring and summer. This work proposes to investigate whether the aerosols from biomass burning may affect the MCS in terms of rainfall over La Plata Basin during spring. Since the aerosol effect is very difficult to isolate because convective clouds are very sensitive to small environment disturbances, detailed analyses using different techniques are used. The binplot, 2D histograms and combined empirical orthogonal function (EOF) methods are used to separate certain environment conditions with the possible effects of aerosol loading. Reanalysis 2, TRMM-3B42 and AERONET data are used from 1999 up to 2012 during September-December. The results show that there are two patterns associated to rainfall-aerosol interaction in La Plata Basin: one in which the dynamic conditions are more important than aerosols to generate rain; and a second one where the aerosol particles have a role in rain formation, acting mainly to suppress rainfall over La Plata Basin.