Cytogenetic instability of dental pulp stem cell lines

Human adult stem cells (hASCs) offer a potentially renewable source of cell types that are easily isolated and rapidly expanded for use in regenerative medicine and cell therapies without the complicating ethical problems that are associated with embryonic stem cells. However, the eventual therapeutic use of hASCs requires that these cells and their derivatives maintain their genomic stability. There is currently a lack of systematic studies that are aimed at characterising aberrant chromosomal changes in cultured ASCs over time. However, the presence of mosaicism and accumulation of karyotypic abnormalities within cultured cell subpopulations have been reported. To investigate cytogenetic integrity of cultured human dental stem cell (hDSC) lines, we analysed four expanded hDSC cultures using classical G banding and fluorescent in situ hybridisation (FISH) with X chromosome specific probe. Our preliminary results revealed that about 70% of the cells exhibited karyotypic abnormalities including polyploidy, aneuploidy and ring chromosomes. The heterogeneous spectrum of abnormalities indicates a high frequency of chromosomal mutations that continuously arise upon extended culture. These findings emphasise the need for the careful analysis of the cytogenetic stability of cultured hDSCs before they can be used in clinical therapies.

Rituals and Knowledge Construction: Ethical Dilemmas on Creating Oppositions

What is knowledge construction for? Mesopotamian rituals were practiced in order to grasp the future and guide war strategies. Nowadays, scientific rules are developed to avoid mysticism-constructing more accurate laws to explain the reality. Both rituals and science were, and usually are, grounded in a conception that to know is to decipher the correct meaning behind the expressive relief of the world. Contemporary studies on anthropology have shown that the opposition between nature and culture is the basis of a number of problems in human sciences aiming to comprehend the intricate relation between body and violence and overcome ethical dilemmas.