Modified CUPRAC spectrophotometric quantification of total polyphenol content in beer samples using Cu(II)/neocuproine complexes

Cu(II)/neocuproine (2,9-dimethyl-1,10-phenanthroline) complexes were utilized for spectrophotometric determination of total polyphenol content in beers. This procedure is based on the reduction of Cu(II) by polyphenols in hydroethanolic medium (pH 7.0) in the presence of neocuproine, yielding Cu(I)/complexes with maximum absorption at 454 nm. The sensitivity of the proposed method was compared with the AOAC method using tannic and gallic acid as standards. The average apparent molar absorptivity, in L cm(-1) mol(-1), of tannic acid (3.50 +/- 0.20) x 10(5) and gallic acid (5.12 +/- 0.21) x 10(4), was twice as high for the proposed method. A lower limit of detection (LOD) (2.9 x 10(-1) mg L-1) was found when tannic acid was used in the proposed method. Additionally, less interference from the most common additives in beers was noticed. Total content of polyphenols was analyzed in 17 Brazilian samples. Results ranged from 35.5 to 556 mg L-1 of tannic acid, with higher values for recovery rates (45.4-118%, mean 85.0%) than for gallic acid. Although tannic acid is a mixture of polygalloyl glucoses, the total polyphenol content found in the samples suggests that tannic acid should be used as a standard. This is the first attempt to use this particular copper complex to quantify total polyphenol content in beer samples. (C) 2012 Elsevier Inc. All rights reserved.

Experimental and Theoretical Study on the One- and Two-Photon Absorption Properties of Novel Organic Molecules Based on Phenylacetylene and Azoaromatic Moieties

This Article reports a combined experimental and theoretical analysis on the one and two-photon absorption properties of a novel class of organic molecules with a pi-conjugated backbone based on phenylacetylene (JCM874, FD43, and FD48) and azoaromatic (YB3p2S) moieties. Linear optical properties show that the phenylacetylene-based compounds exhibit strong molar absorptivity in the UV and high fluorescence quantum yield with lifetimes of approximately 2.0 ns, while the azoaromatic-compound has a strong absorption in the visible region with very low fluorescence quantum yield. The two-photon absorption was investigated employing nonlinear optical techniques and quantum chemical calculations based on the response functions formalism within the density functional theory framework. The experimental data revealed well-defined 2PA spectra with reasonable cross-section values in the visible and IR. Along the nonlinear spectra we observed two 2PA allowed bands, as well as the resonance enhancement effect due to the presence of one intermediate one-photon allowed state. Quantum chemical calculations revealed that the 2PA allowed bands correspond to transitions to states that are also one-photon allowed, indicating the relaxation of the electric-dipole selection rules. Moreover, using the theoretical results, we were able to interpret the experimental trends of the 2PA spectra. Finally, using a few-energy-level diagram, within the sum-over-essential states approach, we observed strong qualitative and quantitative correlation between experimental and theoretical results.

Absortâncias e emitâncias efetivas de superfícies: um método para estimá-las, um modelo experimental e exemplos de suas importâncias

Imperfeições, saliências e reentrâncias existentes nas superfícies típicas das edificações constituem obstáculos que podem alterar significativamente o comportamento delas em relação aos fluxos por radiação. Além de ampliar a área efetiva de troca de calor, criam sombras e reflexões, que não ocorreriam em. Assim, os procedimentos de cálculo, ao invés de considerar as superfícies perfeitamente lisas e planas, deveriam aplicar correções nas propriedades radiativas das superfícies. Denominam-se efetivas as absortâncias e emitâncias que resultam dessas correções. O presente artigo apresenta um método simples desenvolvido para avaliar essas influências e exemplifica a importância das correções acima mencionadas, apresentando resultados de simulações elaboradas no programa EnergyPlus para uma edificação de geometria simples, submetida ao longo de 1 ano ao clima da cidade de Brasília. Adotando-se absortâncias e emitâncias efetivas, os resultados das simulações revelam diferenças de até 2,8 ºC nas temperaturas internas do ar em relação às obtidas desprezando-se as influências que as ondulações das telhas exercem sobre os fluxos radiantes. Considerando o uso de condicionadores de ar, essa diferença pode representar uma variação de cerca de 30% nas estimativas de consumo de energia.