6 resultados para análise da sequência da região ITS

em Repositório Institucional da Universidade Estadual de São Paulo - UNESP


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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Black fungi are able to adapt to extreme environmental conditions, such as: high temperatures, the presence of toxic chemical substances and lack of nutrients. Besides, they are also potential pathogens to humans. The natural environment of many black fungi is still unknown and some studies are being conducted to evaluate the biodiversity of this group and their different habitats. This study aimed to isolate black fungi in domestic environments and facilities, such as toothbrushes, fridge sealing rubbers, bathroom strainers and divisions, windows, wall tiles and bath sponge. For the collection, material surfaces were scratched with a scalpel and the resulting fragments were sewed in Mycosel agar (DifcoTM), supplemented with actidione to inhibit the growth of highly-sporulating fungi. Plates were incubated at 25ºC for three weeks. The 46 isolated fungi were maintained on MA2% slants at 8ºC and cryopreserved at -80ºC. Fungal identification was performed through the analysis of macro and microscopic features and ITS rDNA sequencing. The following black fungi taxa were found: Ascomycota sp., Cladosporium spp., Dothideomycete sp., Exophiala alcalophila, Ochroconis mirabilis and Rhinocladiella atrovirens. Non-melanized fungi were also found, such as Geosmithia sp., Penicillium sp. and Rhodotorula mucilaginosa. The temperature tests showed that isolated black fungi were not able to grow at 37°C, however, this temperature proved to be fungistatic to 43% of them. According to literature, all black fungi isolated in this study are opportunistic pathogens and additional studies are necessary to evaluate the risk that these micro-organisms offer to health, once they were isolated from domestic environments

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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This work deals with the continuation of Scientific Initiation research initiated by Tamura (2012), which draws up a mixture of soil and recycled aggregate analysis for paving of low traffic volume roads using local materials from the Vale do Paraíba region. The main steps of the process were the soil and recycled aggregate characterization, along with grading tests and California Bearing Ratio (CBR) applied to the soil, the recycled aggregate and the mixture of soil and recycled aggregate; aiming their use as base and sub-base in paving urban roads. The results are discussed, emphasizing the mechanical behavior. The current results are compared with the results of IC Tamura research, taking decisions over this job to the final product best result. For a greater understanding of the work in general were described paving, its structure, its components and its physical behavior. It has been intended to emphasize the importance of the mixture of soil and recycled aggregate to meet the quality requirements and compliance with the specifications of current technical standards, in the context of durability, natural materials economy, decreases volume in landfills and cost reduction

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)