Estudo químico dos escapos de Paepalanthus geniculatus Kunth

Eriocaulaceae species are popularly known as sempre-vivas. This family comprising approximately 1.200 species divided into 10 genera, with high endemic levels. Paepalanthus genus has high incidence in the Espinhaço Range region and the report of biological activities. Despite the large number of previous studies with Eriocaulaceae species, Paepalanthus geniculatus has no studies about their scapes, which demonstrates the need for new research to identify their chemical and biological composition. The aim of this work was to study the chemical composition of P. geniculatus ethanolic extract seeking the isolation and identification of metabolites and evaluate the radical scavenging activity of the extract and isolated substances. P. geniculatus were collected in Serra do Cipó-MG in 2013 (Voucher: SANO 3193) and the scapes were dried, crushed and the powder was percolated with ethanol. With the etanolic extract it is carried out a fractionation by gel permeation chromatography, yielding fractions analyzed by TLC assay. The fraction J89 (45,6 mg) was purified by semipreparative HPLC-PDA, resulting in the isolation of substance S1. The fractionation was also performed by medium pressure liquid chromatography, yielding fractions analyzed by TLC assay. The fraction number 4 (92,6 mg) were purified by semipreparative HPLC-PDA resulting in the isolation of substances S2 and S3. The substances S1 and S3 were analyzed by mono and twodimensional NMR, resulting in the identification of 3,4-dihydroxybenzoic acid (protocatechuic acid) and the flavonoid 6-hydroxyquercetin-7-O-β-Dglucopyranoside. The substance S2 were identified by comparison with standards, were it was possible to determine the presence of the flavonoid 6-hydroxy-7- methoxyquercetin-3-O-β-D-glucopyranoside. The evaluation of radical scavenging activity for the extract and the isolated substances using DPPH, showed consistent activity to S1...

Fatores ergonômicos críticos de mochos odontológicos e sua relação com a produtividade e satisfação de cirurgiões dentistas

The purpose of the ergonomics is to generate adequate working conditions, by the correct use of equipment and appropriate anatomic positions. Ergonomics has become essential in the dentist's life while in the dental practice. The misuse of equipment in performing tasks or the wrong choice of equipment can initiate various problems like back pain, bursitis, repetitive strain injury (RSI), limitation of movement, and stress. Using a survey that was implemented with professional dentists linked to Paulista Association of Dental Surgeons (APCD) and graduate students at the Universidade Estadual Paulista (UNESP) in the São José dos Campos (SP) region, this study intends to propose an adequate research instrument to identify ergonomic critical factors of dental stools and their relation to productivity and satisfaction of dentists. The survey was developed with Google Docs Forms tool and was designed by analysis of other studies, existing surveys and other papers in this area. Relevant content were discussed and developed questions about the stool used by dentists in their activities, the occurrence of any problems arising from the exercise of their profession and the views of respondents about the influence of using the dental stool in these cited problems as well as their productivity. Due to obstacles encountered during the project, particularly bureaucratic issues, the survey cannot be implemented as needed, resulting in a test with reduced number of answers. The users' opinion varies according to the stool used, bringing some difficulty to generalizing the answers, but if treated properly, it is possible to obtain relevant conclusions about the main aspects perceived by users in relation to the stool and the needs pointed as most important