NGS y Metagenómica

The Next Generation Sequencing (NGS) allows to sequence the whole genome of an organism, compared to Maxam and Gilbert and Sanger sequencing that only allow to sequence, hardly, a single gene. Removing the separation of DNA fragments by electrophoresis, and the development of techniques that let the parallelization (analysing simultaneously several DNA fragments) have been crucial for the improvements of this process. The new companies in this ambit, Roche and Illumina, bet for different protocols to achieve these goals. Illumina bets for the sequencing by synthesis (SBS), requiring the library preparation and the use of adapters. Likewise, Illumina has replaced Roche because its lower rate of misincorporation, making it ideal for studies of genetic variability, transcriptomic, epigenomic, and metagenomic, in which this study will focus. However, it is noteworthy that the last progress in sequencing is carried out by the third generation sequencing, using nanotechnology to design small sequencers that sequence the whole genome of an organism quickly and inexpensively. Moreover, they provide more reliable data than current systems because they sequence a single molecule, solving the problem of synchronisation. In this way, PacBio and Nanopore allow a great progress in diagnostic and personalized medicine. Metagenomics provide to make a qualitative and quantitative analysis of the various species present in a sample. The main advantage of this technique is the no necessary isolation and growth of the species, allowing the analysis of nonculturable species. The Illumina protocol studies the variable regions of the 16S rRNA gene, which contains variable and not variables regions providing a phylogenetic classification. Therefore, metagenomics is a topic of interest to know the biodiversity of complex ecosystems and to study the microbiome of patients given the high involvement with certain microbial profiles on the condition of certain metabolic diseases.

Periodismo literario y conflicto social en México en las crónicas de Charles Bowden y Judith Torrea

La presente comunicación analiza las relaciones entre periodismo literario y conflicto social a partir de la visión que sobre la violencia, el narcotráfico, los asesinatos, el crimen, las desapariciones etc., tienen las crónicas de Charles Bowden y Judtih Torrea. Bowden es un periodista norteamericano, recientemente fallecido, que ha vivido en primera persona el problema de la violencia a un lado y a otro de la frontera entre México y Estados Unidos. Por su parte, Judith Torrea, es una periodista española, Premio Ortega y Gasset 2010 de periodismo en Internet, afincada en Juárez y cuya obra se ha centrado en las desapariciones de mujeres en Juárez. La perspectiva de ambos profesionales, de procedencia y generación distinta, nos permite abordar el fenómeno del conflicto social mexicano no sólo desde el ángulo del análisis pormenorizado del problema, sino de la visión personal y en muchos casos subjetiva del periodista que se enfrenta in situ, y de manera personal a estas situaciones de conflicto. Nos centraremos para este doble análisis, de un lado La ciudad del crimen: Ciudad Juárez y los nuevos campos de exterminio de la economía global del periodista norteamericano Charles Bowden y Juárez en la sombra: Crónica de una ciudad que se resiste a morir de la periodista española Judith Torrea. Creemos poder contribuir de esta manera al estudio del periodismo literario a través de la crónica como género a partir de las adaptaciones que esta sufre en función del contexto del que trata. Centramos el caso en el estudio de Ciudad Juárez dada la importancia de este caso y su repercusión social y el eco internacional que tiene a lo que se añade el carácter fronterizo del problema y por tanto un fenómeno que posee una singularidad específica. This communication analyses the relationship between literary journalism and social conflict, from the point of view Charles Bowden and Judtih Torrea chronicles have about violence, drug trafficking, murders, crime, disappearances, etc. Bowden is an American journalist, recently deceased, who has experienced on the first hand the problem of violence on the border between Mexico and the United States. Meanwhile, Torrea is a Spanish journalist, awarded in 2010 with the Journalism on the Internet Ortega y Gasset Prize, settled in Juarez conflict and whose work is focused on the disappearances of women in Juarez. The perspectives of both professionals, who are from different origin and generation, allow us to deal with the situation of Mexican social conflict, from the angle of detailed analysis of the problem, and from the journalist’s personal view, often subjective, who had to cope with this reality. For this double analysis, we will focus, firstly, on Murder City: Ciudad Juarez and the Global Economy's New Killing Fields by the American journalist Charles Bowden and, secondly, on City of Juarez: Under the Shadow of Drug Trafficking by the Spanish journalist Judith Torrea. Accordingly, we contribute to the study of literary journalism through the chronicle as genre with the adaptation it suffers depending on the context. Ciudad Juarez is the center of this investigation due to the importance of this case and its social impact and international repercussions. Besides, the border problem contributes to give a specific singularity to this.