Galanin interacts with Neuropeptide Y Y1 receptor in the dentate gyrus of the hippocampus through GALR2/NPYY1R heterodimers

We have shown Galanin(GAL) and Neuropeptide Y Y1(NPYY1) interactions at behavioural, cellular and receptor levels through GALR2/NPYY1R heterodimers in the amygdala. The aim of this work was to analyze GAL/NPYY1R interactions in the Dentate Gyrus(DG) of the Hippocampus, using autoradiographic, in situ hybridization and in situ proximity ligation assay(PLA). Rats(n=6) were sacrificed 15 minutes or 5 hours after icv injections of GAL(3nmol) and DG sections were incubated with NPYY1R agonist [I125]-[Leu31,Pro34]PYY(25 pM) or NPYY1R-33PdATP specific probe, for autoradiography and in situ hybridization respectively. Autoradiograms were analyzed using NIH image analysis system and Student’s unpaired t-test was used. For PLA, DG sections were incubated with anti-GALR2 Rabbit(1:100) and anti-NPYY1R Goat(1:200). PLA signals were detected with PLA PLUS or MINUS probes for rabbit or goat/mouse antibodies. PLA signals were visualized by using a confocal microscope Leica TCS-SL confocal microscope(Leica). We observed that GAL significant increased the NPYY1R agonist [I125]-[Leu31,Pro34]PYY binding in the DG by 20% (p<0,05) and the NPYY1R mRNA expression in the granular layer of DG by 31% (p<0,001). Moreover, PLA-positive red clusters were found specifically in the polymorphic layer and subgranular zone of the DG. No PLA clusters were observed neither in the molecular layer of the DG nor in the corpus callosum, an area that seems to lack of GALR2 receptor. These results demonstrate a novel mechanism of interaction between GAL and NPY1R in the DG at receptor level, probably involving the formation of GALR2/NPYY1R heteroreceptor complexes. Study supported by Junta de Andalucia CVI6476.

Lingüística de corpus y análisis cuantitativo en la gramática (histórica) del español: el caso de las oraciones completivas de sustantivo

El uso de (grandes) corpus textuales como base empírica para el análisis de fenómenos gramaticales ocupa un lugar central dentro de la lingüística contemporánea. La gramática histórica del español no es ninguna excepción, y desde principios del presente milenio los historiadores de la lengua disponen de dos grandes corpus diacrónicos ampliamente usados en el mundo entero, como son el CORDE de la Real Academia Española y el Corpus del español de Mark Davies (2002-). Al lado de los grandes corpus muestras de textos de menor extensión, pero con características relevantes para la investigación en cuestión, también se utilizan como base de análisis empíricos.

Rivalidad de preeminencias entre el cabildo civil y eclesiástico en los cortejos procesionales del Corpus Christi. Antequera (siglo XVI)

Entre 1598 y 1606 la procesión del Corpus en Antequera quedó suspendida por desavenencias entre los miembros del concejo y el clero de la Colegiata por ocupar los mejores puestos y más cercanos a la Sagrada Forma para significar así su posición predominante en la sociedad del momento. Las alteraciones a describir en el presente artículo forman parte del proceso de institucionalización de una fiesta, cuyo culmen estético y simbólico se confirma durante el siglo XVII, bajo la ideología contrarreformista de Trento. Between 1598 and 1606 the Corpus Christi’s procession in Antequera was suspended because of disagreements between the members of the Council and the clergy. These disagreements focused on the matter of who must occupy the best positions in the procession (that is: as close to the Communion Bread as possible). These locations signify the status in that society. The changes described in this paper are part of the process of institutionalization of a festivity, which reach its crowning moment (aesthetically and symbolically) during the seventeenth century, under the ideology of Trento.