Absence of LPA1 receptor results in altered pattern of limbic activation after tail suspension test

Stress serves as an adaptive mechanism and helps organisms to cope with life-threatening situations. However, individual vulnerability to stress and dysregulation of this system may precipitate stress-related disorders such as depression. The neurobiological circuitry in charge of dealing with stressors has been widely studied in animal models. Recently our group has demonstrated a role for lysophosphatidic acid (LPA) through the LPA1 receptor in vulnerability to stress, in particular the lack of this receptor relates to robust decrease of adult hippocampal neurogenesis and induction of anxious and depressive states. Nevertheless, the specific abnormalities in the limbic circuit in reaction to stress remains unclear. The aim of this study is to examine the differences in the brain activation pattern in the presence or absence of LPA1 receptor after acute stress. For this purpose, we have studied the response of maLPA1-null male mice and normal wild type mice to an intense stressor: Tail Suspension Test. Activation induced by behaviour of brain regions involved in mood regulation was analysed by stereological quantification of c-Fos immunoreactive positive cells. We also conducted multidimensional scaling analysis in order to unravel coativation between structures. Our results revealed hyperactivity of stress-related structures such as amygdala and paraventricular nucleus of the hypothalamus in the knockout model and different patterns of coactivation in both genotypes using a multidimensional map. This data provides further evidence to the engagement of the LPA1 receptors in stress regulation and sheds light on different neural pathways under normal and vulnerability conditions that can lead to mood disorders.

Propuesta metodológica para la Educación Patrimonial a través de la puesta en escena de las películas de romanos: el valor artístico y el valor documental

La Educación Patrimonial tiene como objetivo que nuestros alumnos aprendan a legitimar o poner en valor el patrimonio cultural. Las “películas de romanos”, más concretamente la puesta en escena de estos films son un recurso didáctico consolidado; pero la Didáctica de las Ciencias Sociales no cuenta con una metodología que permita dar criterio de certeza al valor histórico-documental así como al valor histórico-artístico de la puesta en escena de las películas de romanos. Proponemos una metodología para la verificación del valor histórico-documental e histórico-artístico de los escenarios de las “películas de romanos”. Heritage Education has as objective that our students learn to qualify or value cultural heritage. The "films about ancient Rome", specifically the scenography of these films are an educational resource consolidated, but Didactics of Social Science doesn't have a didactic methodology which allows for validating to the historical-documentary value as the historical-artistic value of the scenography of "films about ancient Rome". We propose a methodology for the verification of the historical-documentary and historical-artistic value for the scene of these films.