Atividade antimicrobiana de ácidos orgânicos e compostos clorados sobre micro-organismos patogênicos em carne de frango

The current scenario of the Brazilian poultry production is defined by high productivity motivated by exports to markets with elevated levels of sanitary requirement. The work aimed to evaluate the efficacy of chlorinated compounds (chlorine dioxide, dichloro and trichloro) and organic acids (citric, lactic and peracetic acids) in reducing the contamination of poultry by Salmonella spp., mesophiles and enterobacteriaceae. Were isolated 102 strains Salmonella spp. poultry carcass from June to September 2014. Strains were identified by PCR. Was determined the minimum inhibitory concentration (MIC) of antimicrobial compounds for the standard strains of S. Typhimurium, S. Enteritidis and S. Heidelberg. MIC of lactic acid and peracetic acid (20 to 10 g/L) was applied in strains of Salmonella spp. isolated from the slaughter. The MIC of the compounds lactic acid and sodium dichloro was applied in contaminated chiller water with Salmonella (109 CFU/mL) and this was determined Salmonella count in water. Thighs and drumsticks poultry were contaminated with S. Heidelberg (109 UFC/mL) and were applied dichloro (60 mg/L), lactic acid (20 g/L) and sodium hypochlorite (5,0 and 0,5 mg/L) compounds. In the identification by PCR, 93,1% of the strains were identified as Salmonella. For sodium dichloro the MIC was 60 mg/L for 15 minutes to S. Heidelberg and 60 mg/L for 20 minutes for S. Enteritidis. Lactic acid presented MIC of the 5 g/L for 10 minutes to S. Enteritidis 10 g/L for 15 minutes to S. Typhimurium and 20 g/L for 20 minutes to S. Heidelberg. For peracetic acid, MICs were 10 g/L for 10 minutes to S. Typhimurium and S. Heidelberg and 10 g/L for 20 minutes to S. Enteritidis. To citric acid, MICs were 10 g/L for 10 minutes to S. Typhimurium and S. Enteritidis and 25 g/L for 20 minutes to S. Heidelberg. In the isolated Salmonella strains, lactic acid inhibited 97,89% of the strains and peracetic inhibited 100% of the strains. In contaminated chiller water, the compounds reduced the growth of standards strains. When applied to contaminated poultry meat, there was a reduction of Salmonella spp. 1,06 log10 CFU/g relative to the positive control with the use of sodium hypochlorite at 5,0 mg/L, 0,97 log10 CFU/g with dichloro and 0,56 log10 CFU/g with sodium hypochlorite 0,5 mg/L. For mesophiles reduction observed was 0,90 log10 CFU/g relative to the positive control with the use of sodium hypochlorite at 5,0 mg/L, 0,83 log10 CFU/g with dichloro and there isn´t reduction with hypochlorite with sodium 0,5 mg/L. For enterobacteriaceae reduction was 1,0 log10 CFU/g relative to the positive control with the use of sodium hypochlorite at 5,0 mg/L, 0,79 log10 CFU/g with dichloro and 0,22 log10 CFU/g with sodium hypochlorite at 0,5 mg/L. Lactic acid inhibit growth of the microorganisms tested. The data supports the discussions to regulate the use of the technology coadjuvants in the slaughter of poultry.

Um olhar sobre a lei Maria da Penha no Paraná: avanços e desafios

This study aims to analyze the process of implementation of Maria da Penha Law in Paraná from the perception of persons directly involved in combating violence against women in that state. To achieve this goal, the implementation in Curitiba was taken as the main reference, due to its status as capital, being the headquarter of the political powers and the place where are some feminist social movements. We have chosen qualitative approach of interpretative nature as research methodology, because it is a method that allows the analysis of the responses and as a data collection technique. We also have chosen the individual semi-structured interview as interview mode, because it gives greater freedom to the interviewee to discuss the matter, but it is delimited to the study objectives. The research included nine persons, including members of the Judiciary and Public Ministry, public servants and activists. The importance of the study stems from the relevance of the numbers of violence against women in Brazil, and more specifically by the significant occurrence of this kind of acts in the state of Paraná, which currently occupies the 3rd place in the ranking for the most violent states. The paper also discusses gender relations by understanding that violence against women is the result of an asymmetrical power relationship between men and women; human rights because violence is a blatant disregard of women's human rights; on public policies and technologies to confront this form of violence. Among the policies, the Maria da Penha Law is highlighted as one of the most striking examples of public policy for combating violence against women. The research found out which was the participation of Paraná in the discussion and implementation of Maria da Penha Law, identifying relevant facts and people and also what was the repercussion obtained by this law. As for the implementation in Paraná, it was possible to determine progresses, difficulties and challenges of the process. The greatest advances obtained so far are the facilities of: Court of Domestic and Family Violence against Women in Curitiba, Maria da Penha Patrol and Women's Special City Office of Curitiba. As for the difficulties, they are related, among others things, to the physical structure, training of agents, political will, and even cultural issues, which are directly linked to gender issues. Thus it was found that the law is implemented in the state, but there are still several challenges to be achieved, which consist, mainly, of the structure increment for combating violence; awareness and change of mentality of public officials; training of service agents and a greater social participation in combating violence. We concluded that the need for change in gender relations, which is an educational and social evolutionary process and therefore time consuming, is also a challenge.