Rede de conservação on farm para fruteiras nativas da fitofisionomia floresta com araucária: levantamento de informações

Currently, the biodiversity is considered as a powerful food security strategy, ecological and economical for humanity. Brazil is one of the main centers of genetic diversity of native fruit in the world. However, little is known about most of these species. In southwestern Paraná region, this diversity can be found, however, due to human action to increase genetic erosion, it is losing genotypes with potential for use. Thus, the conservation of genetic resources is essential for reduction strategies for damage caused to the environment and the lack of tech-nical information to increase the use of them. This study aimed to obtain information for cre-ating on farm net conservation in four citties this region. This study was carried out in rural properties from Dois Vizinhos, Itapejara D’Oeste, Verê and São Jorge D'Oeste citties, Paraná State, Brazil. It was action plan was established with the rural communities through gathering information with agents considered key in the process, it seeking the greatest number of farm-ers who had their properties in the native fruits as pitanga, jabuticaba, uvaia, cereja-do-mato, guabiroba, guabiju, sete capote, goiaba serrana, araça amarelo e vermelho trees. Semi-structured questionnaire was applied, which concerned issues of presence, handling and use of Myrtaceae fruit trees on their properties and informed consent term. There was a survey of the number and native fruits present in each property. The characterization of each household in terms of diversity handled and used in native fruit was performed. It was realized the soil col-lect in 200 properties with the presence of at least some native fruit tree naturally occurring, in order to determine the preference of the species for the chemical characteristics of the soil. The four citties have native fruits trees in quantity and diversity for the creation of on farm net conservation, with farmers demonstrating knowledge of their role as guardians of this heritage of humanity.

Espectroscopia no infravermelho e método quimiométrico para a pesquisa de antimicrobianos em leite da microrregião de Pato Branco - PR

Considering the social and economic importance that the milk has, the objective of this study was to evaluate the incidence and quantifying antimicrobial residues in the food. The samples were collected in dairy industry of southwestern Paraná state and thus they were able to cover all ten municipalities in the region of Pato Branco. The work focused on the development of appropriate models for the identification and quantification of analytes: tetracycline, sulfamethazine, sulfadimethoxine, chloramphenicol and ampicillin, all antimicrobials with health interest. For the calibration procedure and validation of the models was used the Infrared Spectroscopy Fourier Transform associated with chemometric method based on Partial Least Squares regression (PLS - Partial Least Squares). To prepare a work solution antimicrobials, the five analytes of interest were used in increasing doses, namely tetracycline from 0 to 0.60 ppm, sulfamethazine 0 to 0.12 ppm, sulfadimethoxine 0 to 2.40 ppm chloramphenicol 0 1.20 ppm and ampicillin 0 to 1.80 ppm to perform the work with the interest in multiresidues analysis. The performance of the models constructed was evaluated through the figures of merit: mean square error of calibration and cross-validation, correlation coefficients and offset performance ratio. For the purposes of applicability in this work, it is considered that the models generated for Tetracycline, Sulfadimethoxine and Chloramphenicol were considered viable, with the greatest predictive power and efficiency, then were employed to evaluate the quality of raw milk from the region of Pato Branco . Among the analyzed samples by NIR, 70% were in conformity with sanitary legislation, and 5% of these samples had concentrations below the Maximum Residue permitted, and is also satisfactory. However 30% of the sample set showed unsatisfactory results when evaluating the contamination with antimicrobials residues, which is non conformity related to the presence of antimicrobial unauthorized use or concentrations above the permitted limits. With the development of this work can be said that laboratory tests in the food area, using infrared spectroscopy with multivariate calibration was also good, fast in analysis, reduced costs and with minimum generation of laboratory waste. Thus, the alternative method proposed meets the quality concerns and desired efficiency by industrial sectors and society in general.

Validação de metodologia analítica por CLAE-DAD para determinação de compostos fenólicos e atividade biológica em coprodutos agroindustriais

The southern region of Brazil, especially the states of Parana and Santa Catarina stand out for growing grapes and apples for fresh consumption and in order to add value to these products, process the material for the production of wine, juices and jellies . As a result large quantities of by-products, such as peels, seeds and pulp are produced becoming environmental problems. Studies reuse of these by-products have attracted interest because they have shown a high biological potential, due to the presence of high levels of phenolic compounds, which are associated with a lower incidence of disease caused by oxidative stress, due to its antioxidant, antiinflammatory and antibacterial properties. Currently, few studies are presented on the phenolic composition and biological potential of waste grape variety Bordô (Vitis labrusca) and apple (Malus domestica) Gala variety, cultivated in southern Brazil. Within this context, the objectives of this study were: compare the efficiency of solidliquid and liquid-liquid extraction, perform the optimization and validation of analytical methodology by HPLC-DAD for the separation, identification and quantification of multiclass phenolic compounds, evaluate the activity antioxidant by sequestering methods of free radical 2,2-diphenyl-1 picrilhidrazina (DPPH) and 2,2-azino-bis (3- ethyl-benzthiazoline-6-sulphonic acid) (ABTS) solution, reduction of Fe3+ in Fe2+ method (FRAP), ORAC, RP-HPLC-ABTS online, Rancimat and determination of total phenolics three agro-industrial byproducts, pomace and stems grape Bordô produced in Paraná Southwest region and Gala apple pomace coming from the Santa Catarina West. Optimization and validation of chromatographic method showed satisfactory quality parameters for the compounds of interest and the solidliquid extraction was more efficient in extracting phenolic evaluated. The three byproducts evaluated showed significant levels of phenolic compounds when analyzed by HPLC, especially flavonoids, catechin and epicatechin besides that showed significant antioxidant capacity. The grape stems extract had the highest sequestration capacity of DPPH and ABTS radical and reduced iron, and high content of phenolic compounds. The apple pomace extract showed the best response to the Rancimat method, which indicates a high potential to protect the oil from lipid oxidation, was no significant difference when compared to synthetic antioxidant TBHQ. The results of this study showed that the agro-industrial coproducts analyzed are rich in phenolic compounds of high antioxidant capacity and therefore must be better explored by the food and pharmaceutical industries.

Desenvolvimento e aplicação de modelo de calibração multivariada para determinação de açúcares e ácidos orgânicos em bebidas comerciais utilizando espectroscopia no infravermelho

The routine analysis for quantization of organic acids and sugars are generally slow methods that involve the use and preparation of several reagents, require trained professional, the availability of special equipment and is expensive. In this context, it has been increasing investment in research whose purpose is the development of substitutive methods to reference, which are faster, cheap and simple, and infrared spectroscopy have been highlighted in this regard. The present study developed multivariate calibration models for the simultaneous and quantitative determination of ascorbic acid, citric, malic and tartaric and sugars sucrose, glucose and fructose, and soluble solids in juices and fruit nectars and classification models for ACP. We used methods of spectroscopy in the near infrared (Near Infrared, NIR) in association with the method regression of partial least squares (PLS). Were used 42 samples between juices and fruit nectars commercially available in local shops. For the construction of the models were performed with reference analysis using high-performance liquid chromatography (HPLC) and refractometry for the analysis of soluble solids. Subsequently, the acquisition of the spectra was done in triplicate, in the spectral range 12500 to 4000 cm-1. The best models were applied to the quantification of analytes in study on natural juices and juice samples produced in the Paraná Southwest Region. The juices used in the application of the models also underwent physical and chemical analysis. Validation of chromatographic methodology has shown satisfactory results, since the external calibration curve obtained R-square value (R2) above 0.98 and coefficient of variation (%CV) for intermediate precision and repeatability below 8.83%. Through the Principal Component Analysis (PCA) was possible to separate samples of juices into two major groups, grape and apple and tangerine and orange, while for nectars groups separated guava and grape, and pineapple and apple. Different validation methods, and pre-processes that were used separately and in combination, were obtained with multivariate calibration models with average forecast square error (RMSEP) and cross validation (RMSECV) errors below 1.33 and 1.53 g.100 mL-1, respectively and R2 above 0.771, except for malic acid. The physicochemical analysis enabled the characterization of drinks, including the pH working range (variation of 2.83 to 5.79) and acidity within the parameters Regulation for each flavor. Regression models have demonstrated the possibility of determining both ascorbic acids, citric, malic and tartaric with successfully, besides sucrose, glucose and fructose by means of only a spectrum, suggesting that the models are economically viable for quality control and product standardization in the fruit juice and nectars processing industry.

Modelo para distribuição de recursos nos municípios brasileiros baseado na lei de diretrizes orçamentárias, análise multicritério e programação linear

The municipal management in any country of the globe requires planning and allocation of resources evenly. In Brazil, the Law of Budgetary Guidelines (LDO) guides municipal managers toward that balance. This research develops a model that seeks to find the balance of the allocation of public resources in Brazilian municipalities, considering the LDO as a parameter. For this using statistical techniques and multicriteria analysis as a first step in order to define allocation strategies, based on the technical aspects arising from the municipal manager. In a second step, presented in linear programming based optimization where the objective function is derived from the preference of the results of the manager and his staff. The statistical representation is presented to support multicriteria development in the definition of replacement rates through time series. The multicriteria analysis was structured by defining the criteria, alternatives and the application of UTASTAR methods to calculate replacement rates. After these initial settings, an application of linear programming was developed to find the optimal allocation of enforcement resources of the municipal budget. Data from the budget of a municipality in southwestern Paraná were studied in the application of the model and analysis of results.

Características químicas do solo e desempenho de culturas após quatro anos de aplicações de cama de aviário em diferentes épocas e níveis

Due to the high supply and its attractive cost, the poultry litter has been used in the southwestern region of Parana to the improvement of soil fertility seeking greater production of grains and pastures. However, the use without technical knowledge can minimize the benefits of poultry litter or even cause undesirable effects on soil, environmental pollution and also productivity losses in the used crops. The objective of this study was to evaluate the influence of different times of poultry litter application, predating the winter crop, associated with increasing levels, about soil chemical properties, release of nutrients and crop performances in four consecutive years (2011-2014). In the first three years the experimental design was randomized blocks with a split plot system and four replications. In the main plots were tested four poultry litter application times preceding the wheat production: 0, 15, 30 and 45 days before sowing (DAS); in the subplots were applied four poultry litter levels (wet basis): 0, 4, 8 and 12 Mg ha-1. Last year one more subdivision of plots was done, evaluating the use or not of nitrogen in coverage in wheat, at a dose of 100 kg N ha-1. The wheat cultivar used in the four years was the BRS 220. In three years it was evaluated the residual effect on soybean production (cultivar - BMX Turbo RR) and in one year on the beans. The chemical soil attributes were evaluated at four depths 0-2,5cm, 2,5-5cm, 5-10cm and 10-20cm, and also the rate of decomposition and nutrient release of poultry litter and the crop productivity. The different times of application concerning the poultry litter had little influence on the studied variables, demonstrating that the producer does not need to have a specific date (before planting) to the application of poultry litter. Potassium was fully released 60 days after the allocation of litter bags into the field; for nitrogen and phosphorus the release was slower. The use of increasing levels of poultry litter increased the levels of various soil elements, highlighting the potassium which reached 20 cm deep in the second year of evaluation. The increase in pH and in the base saturation occurred only in the upper layers, while the phosphorus reached 10 cm deep in the third year of the study. It was observed increased pH and base saturation. The use of increasing doses of poultry litter contributed to the wheat plant nutrition, significantly increasing the weight of a thousand grains, and the grain yield of wheat in all the evaluated years; the nitrogen fertilization in coverage also had significant effect for the fourth evaluated year. Also there was a significant response from the residual effect of poultry litter for crops planted in summer for both soybeans and beans.

Proposta de um modelo de gestão de custos para pequenas propriedades rurais familiares com foco na tomada de decisão

This work was general-purpose, develop a proposal of a theoretical model of decisionmaking with a focus on management of small family farms costs, which enables support for decision making. And the following objectives: i) develop a structured methodology, which allows to form a literary basis to provide scientific support for the implementation of research; ii) develop based on the literature the dimensions and variables of the necessary models to propose an application and iii tooling) to implement the proposed model within the dimensions and variables, and validate every stage background and perform the necessary conclusions to verify the effectiveness of applied model. In terms of methodology, we used a structured methodology, which allowed forming a bibliographic portfolio of 29 articles, and through the research constructs developed, based on an existing model, an activity segmentation model for aid farmer of small family farms in the decision-making process with emphasis on cost management. The model was applied in six family farms in the South West region of Parana and Santa Catarina West. With regard to the search results, it was identified that the model can be applied to the specific context for which it was created. It was also possible to identify that the proposed model was valid and relevant to aid in the management of family farms by identifying, through the targeting of productive activities, investment priorities guided by the balance between managing costs and return activities. Moreover, possible to target the activities of six surveyed properties, demonstrating that the property 02, has the shape of more complex segmentation should be divided into three groups of activities, which can be conducted in parallel without any restrictions between activities. Other properties have the segmentation of the simplest activities, allowing viewing in this way that there are activities of groups that require prioritizing investments. Specifically the property 01 and 04 have the highest priority investment target groups, the most prominent activities of groups representing respectively 49.32% and 47.40%, which are represented by grain production activities on the property 01 and grain production, beef cattle and eggs on the property 04.