Wild carrot differentiation in Europe and positive selection at DcAOX1 gene

By definition, the domestication process leads to an overall reduction of crop genetic diversity. This lead to the current search of genomic regions in wild crop relatives (CWR), an important task for modern carrot breeding. Nowadays massive sequencing possibilities can allow for discovery of novel genetic resources in wild populations, but this quest could be aided by the use of a surrogate gene (to first identify and prioritize novel wild populations for increased sequencing effort). Alternative oxidase (AOX) gene family seems to be linked to all kinds of abiotic and biotic stress reactions in various organisms and thus have the potential to be used in the identification of CWR hotspots of environment-adapted diversity. High variability of DcAOX1 was found in populations of wild carrot sampled across a West-European environmental gradient. Even though no direct relation was found with the analyzed climatic conditions or with physical distance, population differentiation exists and results mainly from the polymorphisms associated with DcAOX1 exon 1 and intron 1. The relatively high number of amino acid changes and the identification of several unusually variable positions (through a likelihood ratio test), suggests that DcAOX1 gene might be under positive selection. However, if positive selection is considered, it only acts on some specific populations (i.e. is in the form of adaptive differences in different population locations) given the observed high genetic diversity. We were able to identify two populations with higher levels of differentiation which are promising as hot spots of specific functional diversity.

Relatório da prática de ensino supervisionada em educação física na Escola Secundária/3 André de Gouveia

O presente Relatório de Estágio surge no âmbito da Prática de Ensino Supervisionada, que decorreu no ano letivo de 2011/2012 na Escola Secundária André de Gouveia, em Évora. O objetivo do mesmo é aliar à componente científica que foi adquirida no Mestrado de Educação Física nos Ensino Básico e Secundário o trabalho desenvolvido enquanto estudante estagiário. Para tal, o meu trabalho foi auxiliado pelo Programa Nacional de Educação Física e pelo Projeto Educativo em vigor na Escola. Assim, foi realizada uma descrição do trabalho realizado durante o ano letivo e a respetiva reflexão crítica, tendo em conta quatro dimensões profissionais do professor, nomeadamente: dimensão profissional social e ética, dimensão de desenvolvimento do ensino e da aprendizagem, dimensão da participação na escola e relação com a comunidade e, por último, a dimensão do desenvolvimento profissional ao longo da vida; REPORT OF THE SUPERVISED TEACHING PRACTICE OF PHYSICAL EDUCATION IN THE SECUNDARY SCHOOL/3 ANDRÉ DE GOUVEIA Abstract: This report comes under the Supervised Teaching Practice which took place in the school year 2011/2012 at Secondary School André de Gouveia, in Évora.The purpose of it is to combine the scientific component which was acquired in the Master of Physical Education in the Elementary and Secondary Education the work as a trainee student. To this end, my work was aided by the National Program of Physical Education and the Educational Project used in the school. Thus, a critical reflection of the school year was made, taking into account four professional dimensions of training of future teachers, namely: social professional and ethical dimension, development dimension of teaching and learning, participation in school size and relationship with the community and, finally, the dimension of professional development throughout life.