Effect of trees in the soil nutrient concentration and pasture produtivity in Mediterranean silvo-pastoral systems

Silvo-pastoral are mixed systems of trees and grass, which have been proposed as a means to extend the benefits of forest to farmed land. Agro-forestry systems under semi-arid Mediterranean conditions, called montados in Portugal and dehesas in Spain, cover substantial areas in the world. These silvo-pastoral systems are the most extensive European agro-forestry system, as they cover 3.5–4.0 Mha in Spain and Portugal. Long-term studies are essential to assess the magnitude of the temporal nutrient flow dynamics in terrestrial ecosystems and to understand the response of these systems to fertilizer management. In order to implement the conservation task and recovery of resources through silvo-pastoral systems it is necessary to know and correct potential limiting factors, especially the soil factor, and this requires agronomic knowledge as well as the implmentation of the available new technologies. In this context, this task aims at a better understanding of the contribution of the two components of montado ecosystem (trees and herbaceous vegetation) on the soil nutrient and water dynamics, that allow for the interpretation of the variability of pasture dry matter yield and help the farmer in the management of tree density. Collaterally the task will evaluate and calibrate new technologies that simplify the monitoring of soil, grassland, trees and grazing animals.

Análise da paisagem e das alterações de uso/ocupação do solo no Lubango e arredores

A cartografia temática surge como um aliado indispensável ao estudo e conhecimento do estado da paisagem e dos ecossistemas terrestres. Angola é um país com uma enorme lacuna em termos de informação cartográfica para suporte a diversas actividades desenvolvidas em várias áreas. É por isso necessária informação sobre a superfície terrestre que registe as alterações temporais ocorridas nos ecossistemas e identifique os factores que estão na sua origem de forma a implementar medidas que permitam uma gestão mais sustentável do seu enorme território. O presente estudo foi realizado no sector Sudoeste de Angola, abrangendo os municípios do Lubango, Humpata e Chibia, com uma área aproximada de 9600 km2. Os objectivos foram: a) cartografar o coberto do solo e monitorizar as alterações ocorridas na área de estudo no período 1990-2010, b) analisar e interpretar as alterações da estrutura da paisagem nos últimos 20 anos usando cartografia de ocupação do solo, produzida recorrendo a imagens de satélite, e métricas da paisagem, e c) quantificar e examinar a relação entre a fragmentação da paisagem e os factores geradores. A metodologia utilizada no estudo é composta por duas partes, sendo a primeira dedicada às operações de processamento e interpretação de imagens de satélite Landsat e, a segunda dedicada à produção de novas métricas de fragmentação da paisagem com recurso à ferramenta SIG utilizando operações de álgebra de mapas. O comportamento da fragmentação é analisado, à luz do efeito dos fatores ambientais e socioeconómicas ao nível da região. Os resultados obtidos, através da aplicação de técnicas de deteção remota e usando imagens Landsat TM e ETM+, permitiram a elaboração de mapas de coberto do solo, onde se distinguiram 8 classes temáticas e espectralmente diferentes. Verificou-se em termos globais que aproximadamente 38% da área sofreu algum tipo de alteração no período estudado, sendo as classes de miombo e áreas cultivadas as que mais alterações sofreram. A fragmentação da paisagem foi avaliada através da implementação de novas métricas, mostrando os resultados, que no período 1990-2000, a fragmentação foi superior à que se verificou entre 2000-2010. A nível espacial, a dinâmica de fragmentação foi mais acentuada, entre 1990-2000, na Humpata e, entre 2000-2010, no Lubango; ANALYSIS OF LANDSCAPE AND LAND USE LAND COVER CHANGE IN LUBANGO AND SURROUNDINGS ABSTRACT: Thematic cartography shows up as an essential ally in the study and knowledge of the state of landscapes and terrestrial ecosystems. Angola is a country with an enormous lack of cartographic information to support the several activities carried out in a variety of areas. This is the reason information on the earth’s surface is necessary, registering alterations which occur over time in ecosystems and identifying the associated factors in its origin, so as to implement metrics that allow a more sustainable management of its enormous territory. This study was carried out in the Southeast of Angola in the Lubango, Humpata and Chibia Municipalities, with an area of approximately 9600 km2. Our objectives were the following: a) land cover mapping and land cover changes monitoring over the period 1990 to 2010 using Landsat images, b) to analyze and interpret landscape structures changes using land cover maps, and landscapes metrics, and c) to quantify and to examine the relationship between landscape fragmentation and its drivers. The methodology developed in the study has two parts, the first includes Landsat satellite images processing and interpretation and, the second the production of new landscape fragmentation metrics with support to a GIS tool and algebraic mapping operations. The fragmentation behavior is analyzed, taking into account the effect of environmental and socioeconomic factors at a regional level. The results allowed obtaining land cover maps, in which 8 spectrally different thematic classes were distinguished. It was observed that 38% of the area suffered some type of alteration in the studied period, with higher changes observed in the classes of miombo and agriculture. Landscapes fragmentation results, evaluated through the implementation of new metrics, show that, values are greater in the period 1990-2000 than in 2000-2010. At municipality level, fragmentation dynamics were more accentuated in Humpata between 1990 -2000 and in Lubango between 2000 -2010.

Development of molecular approaches to assess the diversity and abundance of marine nematodes assemblages for assessment of Good Environmental Status of the estuarine and marine ecosystems

In Europe, the concerns with the status of marine ecosystems have increased, and the Marine Directive has as main goal the achievement of Good Environmental Status (GES) of EU marine waters by 2020. Molecular tools are seen as promising and emerging approaches to improve ecosystem monitoring, and have led ecology into a new era, representing perhaps the most source of innovation in marine monitoring techniques. Benthic nematodes are considered ideal organisms to be used as biological indicator of natural and anthropogenic disturbances in aquatic ecosystems underpinning monitoring programmes on the ecological quality of marine ecosystems, very useful to assess the GES of the marine environment. dT-RFLP (directed Terminal-Restriction Fragment Length Polymorphism) allows to assess the diversity of nematode communities, but also allows studying the functioning of the ecosystem, and combined with relative real-time PCR (qPCR), provides a high-throughput semi-quantitative characterization of nematode communities. These characteristics make the two molecular tools good descriptors for the good environmental status assessment. The main aim of this study is to develop and optimize the dT-RFLP and qPCR in Mira estuary (SW coast, Portugal). A molecular phylogenetic analysis of marine and estuarine nematodes is being performed combining morphological and molecular analysis to evaluate the diversity of free-living marine nematodes in Mira estuary. After morphological identification, barcoding of 18S rDNA and COI genes are being determined for each nematode species morphologically identified. So far we generated 40 new sequences belonging to 32 different genus and 17 families, and the study has shown a good degree of concordance between traditional morphology-based identification and DNA sequences. These results will improve the assessment of marine nematode diversity and contribute to a more robust nematode taxonomy. The DNA sequences are being used to develop the dT-RFLP with the ability to easily process large sample numbers (hundreds and thousands), rather than typical of classical taxonomic or low throughput molecular analyses. A preliminary study showed that the digest enzymes used in dT-RFLP for terrestrial assemblages separated poorly the marine nematodes at taxonomic level for functional group analysis. A new digest combination was designed using the software tool DRAT (Directed Terminal Restriction Analysis Tool) to distinguished marine nematode taxa. Several solutions were provided by DRAT and tested empirically to select the solution that cuts most efficiently. A combination of three enzymes and a single digest showed to be the best solution to separate the different clusters. Parallel to this, another tool is being developed to estimate the population size (qPCR). An improvement in qPCR estimation of gene copy number using an artificial reference is being performed for marine nematodes communities to quantify the abundance. Once developed, it is proposed to validate both methodologies by determining the spatial and temporal variability of benthic nematodes assemblages across different environments. The application of these high-throughput molecular approaches for benthic nematodes will improve sample throughput and their implementation more efficient and faster as indicator of ecological status of marine ecosystems.

Pine wilt disease: a worldwide threat to forest ecosystems

The pinewood nematode (PWN), Bursaphelenchus xylophilus, the causal agent of pine wilt disease (PWD), is a serious pest and pathogen of forest tree species, in particular among the genus Pinus. It was first reported from Japan in the beginning of the XXth century, where it became the major ecological catastrophe of pine forests, with losses reaching over 2 million m3/ year in the 1980s. It has since then spread to other Asian countries such as China, Taiwan and Korea, causing serious losses and economic damage. In 1999, the PWN was first detected in the European Union (EU), in Portugal, and immmediately prompted several government (national and EU) actions to assess the extent of the nematode’s presence, and to contain B. xylophilus and its insect vector (Monochamus galloprovincialis) to an area with a 30km radius in the Setúbal Peninsula, 20 km south of Lisbon. International wood trade, with its political as well as economic ramifications, has been seriously jeopardized. The origin of the population of PWN found in Portugal remains elusive. Several hypotheses may be considered regarding pathway analysis, basically from two general origins: North America or the Far East (Japan or China). World trade of wood products such as timber, wooden crates, palettes, etc… play an important role in the potential dissemination of the pinewood nematode. In fact, human activities involving the movement of wood products may be considered the single most important factor in spreading of the PWN. Despite the dedicated and concerted actions of government agencies, this disease continues to spread. Very recently (2006), in Portugal, forestry and phytosanitary authorities (DGRF and DGPC) have announced a new strategy for the control and ultimately the erradication of the nematode, under the coordination of the national program for the control of the pinewood nematode (PROLUNP). Research regarding the bioecology of the nematode and insect as well as new detection methods, e.g., involving real-time PCR, has progressed since 1999. International agreements (GATT, WTO) and sharing of scientific information is of paramount importance to effectively control the nematode and its vector, and thus protect our forest ecosystems and forest economy.

List of the terrestriaL nematodes (nematoda) from Azores

Os nemátodes são um grupo de invertebrados, não segmentados que formam um Filo (Nematoda) bem definido e claramente distinto dos outros grupos de organismos. Este Filo constitui um dos grupos animais mais disseminados no planeta, e em termos de número de indivíduos os nemátodes são o grupo animal mais abundante na Terra: quatro em cada cinco animais da Biosfera são nemátodes. Apesar de microscópicos, os animais multicelulares que constituem este grupo são capazes de explorar uma enorme variedade de habitats, nos mares, nas águas doces, nos solos, como parasitas de animais ou de plantas, ou mesmo em condições extremas, como nos solos secos da Antárctida ou em fontes termais (Baldwin et al. 1999). A actual informação sobre a nematofauna do arquipélago dos Açores encontra-se fraccionada e espalhada em diversas publicações científicas, mas igualmente em dados ainda não publicados (Sturhan, comunicação pessoal). Entre as diversas publicações de nemátodes para os Açores, há que salientar os trabalhos realizados por Sturhan (1973, 1975, 1983) e Macara (1994), que muito contribuíram para o conhecimento e distribuição actual das espécies de vida livre e parasitas de plantas; bem como os trabalhos de Afonso-Roque (1995) e Casanova et al. (1996) relativamente a espécies parasitas de animais, reportadas para as diferentes ilhas do arquipélago. A lista de nemátodes apresentada neste capítulo, tem como base as publicações conhecidas para o arquipélago (ver listagem de publicações até 2010 em www. naturdata.com), bem como o registo de espécies assinaladas pela primeira vez para o arquipélago (Sturhan, comunicação pessoal). A classificação utilizada baseia-se na recente revisão da sistemática e filogenia proposta por De Ley & Blaxter (2002) para todo o Filo, até ao nível da Família. A classificação dos restantes taxa segue o critério utilizado para a fauna terrestre da Fauna Europaea (http://www. faunaeur.org). É apresentada a distribuição das espécies nas nove ilhas dos Açores, usando-se a seguinte simbologia: COR – Corvo; FLO – Flores; FAI – Faial; PIC – Pico; GRA – Graciosa; SJG – São Jorge; TER – Terceira; SMG – São Miguel e SMR – Santa Maria.