Wild carrot differentiation in Europe and positive selection at DcAOX1 gene

By definition, the domestication process leads to an overall reduction of crop genetic diversity. This lead to the current search of genomic regions in wild crop relatives (CWR), an important task for modern carrot breeding. Nowadays massive sequencing possibilities can allow for discovery of novel genetic resources in wild populations, but this quest could be aided by the use of a surrogate gene (to first identify and prioritize novel wild populations for increased sequencing effort). Alternative oxidase (AOX) gene family seems to be linked to all kinds of abiotic and biotic stress reactions in various organisms and thus have the potential to be used in the identification of CWR hotspots of environment-adapted diversity. High variability of DcAOX1 was found in populations of wild carrot sampled across a West-European environmental gradient. Even though no direct relation was found with the analyzed climatic conditions or with physical distance, population differentiation exists and results mainly from the polymorphisms associated with DcAOX1 exon 1 and intron 1. The relatively high number of amino acid changes and the identification of several unusually variable positions (through a likelihood ratio test), suggests that DcAOX1 gene might be under positive selection. However, if positive selection is considered, it only acts on some specific populations (i.e. is in the form of adaptive differences in different population locations) given the observed high genetic diversity. We were able to identify two populations with higher levels of differentiation which are promising as hot spots of specific functional diversity.

Qualidade de vida e aptidão física funcional dos idosos do concelho de Évora

Já na antiguidade o Homem se debruçava sobre a temática do envelhecimento, todavia os anos passaram e este fenómeno constitui-se como um dos assuntos da ordem do dia que nos abrange a todos, porque todos queremos vivenciar o prazer de envelhecer, à semelhança dos nossos antepassados. Porém estes procuravam o elixir de uma vida eterna e nós ambicionamos o elixir de uma longa vida com qualidade. Objectivo: Avaliar a qualidade de vida e aptidão física funcional dos idosos residentes do Concelho de Évora em instituições da rede solidária e nos seus domicílios com idade igual ou superior a 75 anos. Métodos: Após a selecção aleatória e tendo por base os critérios de exclusão (sem défice cognitivo avaliado pelo MMS e sem problemas físicos que impedissem de realizar os testes funcionais) aceitaram participar no estudo 396 idosos do Concelho de Évora dos quais 196 se encontram institucionalizados nos lares da rede solidária e 200 residem nos seus domicílios. Os instrumentos de avaliação utilizados foram o EASYcare e a bateria de testes Rikly Jones- Senior Fitness Test. Conclusões: Os idosos não institucionalizados apresentam melhor qualidade de vida e aptidão física funcional face aos idosos institucionalizados. Os níveis de incapacidade do grupo de idosos são baixos, o que revela que estamos perante um grupo maioritariamente independente. Por sua vez os homens apresentam melhor auto­ percepção da sua qualidade de vida e melhor desempenho nos testes físicos realizados. Com o processo de envelhecimento é notório uma avaliação inferior nas diferentes áreas da qualidade de vida por parte dos idosos assim como um pior desempenho nos testes físicos. Verificou-se também que existem correlações significativas entre as áreas da qualidade de vida avaliadas e os parâmetros de aptidão física funcional considerados, estando estes interrelacionados. ABSTRACT: Introduction: Already in the antiquity Man was dealing with the aging topic, however the years passed and this issue is as one of the topics on the agenda that covers everyone, because we all want to experience the pleasure of growing old, like of our ancestors. Although they sought the elixir of eternal life and we are aiming the elixir of long life with quality. Aim: To evaluate the quality of life and functional fitness of elderly aged over 75 years, residents of the Municipality of Évora in the network institutions in their households. Methods: After random selection and based on the exclusion criteria (no cognitive impairment assessed by MMS and no conditions that hamper them from performing functional tests) agreed to participate in the study 396 elderly from Évora's Municipality of which 196 are institutionalized in nursing homes network of solidarity and 200 reside in their homes. The assessment instruments used were the EASYcare and battery of tests Rikly Jones- Senior Fitness Test. Conclusions: The non-institutionalized elderly have a better quality of life and functional fitness in relation to the institutionalized elderly. The levels of disability in the elderly group are low, indicating that this is a group mostly independent. ln tum the men have better self-perception of their quality of life and better performance in physical tests performed. With the aging process is an assessment of less known in different areas of life quality for elderly people as well as a poorer performance in physical tests. There was also that there are significant correlations between the areas of quality of life and evaluated the parameters of functional fitness considered and these interrelated.