Computerized key to the genus Bursaphelenchus Fuchs, analysis of species clusters based on morphology, using information of insect vectors and associated plants, with a revision of the genus.

The genus Bursaphelenchus includes B. xylophilus (Steiner et Buhrer, 1934) Nickle, 1981, which is of world economic and quarantine importance. Distinction among several species of the pinewood nematodes species complex (PWNSC) is often difficult. Besides standard morphology, morphometrics and molecular biology, new tools are welcome to better understand this group. The computerized (or e-) key of this genus, presented in this communication, includes 74 species (complete list of valid species of the world fauna) and 35 characters, that were used by the taxonomic experts of this group, in the original descriptions. Morphology of sex organs (male spicules and female vulval region) was digitized and classified to distinguish alternative types. Several qualitative characters with overlapping character states (expressions) were transformed into the morphometric indices with the discontinuous ranges (characters of ratios of the spicule dimensions). Characters and their states (expressions) were illustrated in detail and supplied by brief user-friendly comments. E-key was created in the BIKEY identification system (Dianov & Lobanov, 1996-2004). The system has built-algorithm ranging characters depending on their diagnostic values at each step of identification. Matrix of species and the character states (structural part of the e-key database) may be easily transformed using statistical packages into the dendrograms of general phenetic similarities (UPGMA, standard distance: mean character difference). It may be useful in the detailed analysis of taxonomy and evolution of the genus and in its splitting to the species groups based on morphology. The verification of the dendrogram using the information on the species links with insect vectors and their associated plants, provided an opportunity to recognize the five clusters (xylophilus, hunti, eremus sensu stricto, tusciae and piniperdae sensu stricto), which seem to be the natural species groups. The hypothesis about the origin and the first stages of the genus evolution is proposed. A general review of the genus Bursaphelenchus is presented.

Review of the genus Bursaphelenchus Fuchs, 1937 (Nematoda, Aphelenchida) with some conclusions on the host-parasite and vector-parasite evolution.

INTRODUCTION AND GOALS: Genus Bursaphelenchus includes several pests of the world importance for the rural economy, the most dangerous are the Bursaphelenchus xylophilus (the pinewood nematode caused decline of the pine trees in south Asia and in one spot area in Europe, Portugal, Peninsula de Setubal) and the Bursaphelenchus cocophilus, causing the decline of coco-palm plantations in Carribean and Latin American regions. The peculiarity of the host-parasite association of the genus that the nematode life cycle includes three trophic components: plant (mostly a tree), insect vector and a fungus. Goals of the presentation is to list all species of the world fauna and all efficient diagnostic characters, then create the identification tool and analyze the similarity of species and possible ways and causes of the host-parasite evolution of the group. RESULTS: Complete list of species with synonymy and a catalogue of all efficient diagnostic characters with their states, selected from papers of the most experienced taxonomists of the genus, are given for the genus Bursaphelenchus. List of known records of Bursaphelenchus species with names of natural vectors and plants and their families is given (for world pests the most important groups of trees and insects are listed). The tabular, traditional and computer-aided keys are presented. Dendrograms of species relationships (UPGMA, standard distance: mean character difference) based on all efficient taxonomic characters and separately on the spicule characters only, are given. Discussion whether the species groups are natural or purely diagnostic ones is based on the relationships dendrograms and the vector and associated plant ranges of Bursaphelenchus species; the xylophilus species group (B. xylophilus, B. abruptus, B. baujardi, B. conicaudatus, B. eroshenkii, B. fraudulentus, B. kolymensis, B. luxuriosae; B. mucronatus), the hunti group (B. hunti, B. seani, B. kevini and B. fungivorus) are probably the natural ones. CONCLUSIONS: The parasitic nematode association includes three trophic components: plant, insect vector and fungus. The initial insect-plant complex Scolytidae-Pinaceae is changeable and only in rare occasions the change of the preferred vector to Cerambycidae (the xylophilus group), Hymenoptera (the hunti group) led to formation of the natural species-groups. From the analysis it is clear that although the vector range is changeable it is comparatively more important for the evolution of the genus Bursaphelenchus than associations with plants at the family level. Data on the fungi species (3rd component in natural Bursaphelenchus associations) are insufficient for the detailed comparative analysis.

Seláceos do Farol das Lagostas (Bacia do Cuanza, Angola)

O presente trabalho consiste na apresentação da diversidade de seláceos do Farol das Lagostas pertencente à Bacia do Cuanza. Os condrichthyes são componentes comuns da fauna aquática desde o Paleozoico. No entanto, devido à natureza cartilaginosa do esqueleto, o registo paleontológico dos mesmos restringe-se, basicamente, às partes mineralizadas como dentes, dentículos dérmicos e espinhas cefálicas O lugar Farol das Lagostas contém uma fauna de seláceos relativamente rica e variada, representada neste estudo por cerca de 1.000 dentes isolados, além de outras peças esqueléticas. Foram identificados e descritos quarenta e quatro taxa, pertencentes às seguintes ordens: Hexanchiformes, Squaliformes, Pristiophoriformes, Squatiniformes, Lamniformes, Carcharhiniformes, Rajiformes e Myliobatiformes. Trata-se de uma diversidade faunística cuja maioria dos géneros possui representantes atuais. Os Carcharhiniformes representam 53% dos taxa identificados. Estão representadas as famílias Scyliorhinidae, Triakidae, Hemigaleidae, Carcharhinidae e Sphyrnidae, com 18 espécies. Verifica-se a presença de formas bentónicas e nectónicas que indicam condições térmicas moderadas, quentes e tropicais a temperadas, que habitam frequentemente zonas costeiras. A relação faunística identificada corresponde a uma zona litoral. Ora, considerando o conjunto de dados em face da presença de fauna pelágica e dos grandes predadores, especialmente Isurus hastalis e Carcharocles megalodon, podemos admitir que existisse então um Golfo relativamente largo de uma faixa Atlântica aberta (Antunes & Balbino, 2004); ABSTRACT: Selachians from Farol the Lagostas (Cuanza Basin, Angola) The present work consists of the presentation of diversity of selachians from Farol das Lagostas, which belongs to the Cuanza Basin. The condrichthyes are a part of aquatic fauna since the Paleozoic. However, due to the nature of the cartilaginous skeleton, the paleontological registration of the same ones is restricted to mineralized parts like teeth, dermal denticles and cephalic spines. The of formation, Farol das Lagostas has a fauna of selachian relatively rich and diverse, represented in this study for around 1.000 isolated teeth, and other skeletal parts. Forty four taxa were described and identified, belonging to the following orders: Hexanchiformes, Squaliformes, Pristiophoriformes, Squatiniformes, Lamniformes, Carcharhiniformes, Rajiformes e Myliobatiformes. It’s a faunal diversity whose most genera have current representatives. The Carcharhiniformes represente 53% of the identified taxa. The represented families are Scyliorhinidae, Triakidae, Hemigaleidae, Carcharhinidae e Sphyrnidae, with 18 species. There is presence of benthic and nektonic forms that indicate moderate thermal conditions, the temperate and tropical hot, often inhabit coastal areas. The identified faunal relation corresponds to a coastal zone. So, considering the data set, due to the presence of pelagic fauna and large predators, especially Isurus hastalis e Carcharocles megalodon, we can admit that then there was a relatively large Gulf of open Atlantic range (Antunes & Balbino, 2004).

The flora and vegetation of rocky outcrops in three municipalities in the northern region of Ceará, Brazil: phytosociological characterization

This study aims to identify the flora and vegetation of rocky outcrops of low altitude and confined in the municipalities of Sobral, Groaíras and Santa Quitéria (Ceará state, Brazil), to propose a phytosociological classification for the xerophilous communities. We selected five stations in areas with high proportion of bare rock (> 80%), and the field work were conducted in March 2014 and 2015 respectively (3º 56’ S and 40º 23’ W, 4º 01’ S and 40º 05’ W, 4º 07’’ S and 40º 08’ W, 4º 09’ S and 40º 09’ W and 4º 03’ S and 40º 00’ W). Floristic relevés were made following the Braun-Blanquet classic sigmatist method. The minimum areas of the floristic relevés vary between 8 e 16 m². All the plant species growing in cracks, crevices and vegetation "spots" that can be found in these habitats were identified. The classification of the relevés was made through the Twinspan. The floristic list is composed of 89 species, distributed in 61 genera and 29 families. Fabaceae was the most representative in species richness, 20 species, followed by Poaceae (10 spp.), Euphorbiaceae (7 spp.) and Convolvulaceae (6 spp.). 22 Brazilian endemisms have been identified. Based in the phytosociological analysis and in the classification results we identified five groups and two communities can be clearly distinguished: community of Pilosocereus gounellei FA.C.Weber) Byles & Rowley and Encholirium spectabile Mart. ex Schult. & Schult.f. and the community of Crateva tapia L. and Combretum leprosum Mart..

The flora and communities Carnaubal Palm Forest in the northern region of Ceará - Brazil

The “dicótilo-palmácea” mixed forest is found in the fluvial plains (floodplains) of watercourses on the Ceará semiarid region (Brazil), distinguishing from the surrounding vegetation (caatinga) by the prevalence of larger tree species. In the river’s margins, presenting high variability in the extension of the riverbanks, arise floodplains in pedologic complexes mainly composed by neossols and argissols, resulting from the deposition of sediments. In these areas of high fertility soils and subjected to flooding during part of the year, it develops a particular type of riparian vegetation dominated by carnauba palm tree (Copernicia prunifera (Mill.) H.E. Moore) forming a particular type of riparian forest, designated by carnaubal palm forest. We aimed to carry out floristic and phytosociological surveys of carnauba palm forests located in the northern region of Ceará. The classical sigmatist method of Braun-Blanquet was applied and classification analysis (Twinspan) was perfomed. The field work occurred in March 2014 and 2016 in eight areas: Fazenda Pedra Branca (03º 37’ 10’’ S e 40º 18’ 30’’ W, 104 m asl), Vale do Rio Bom Jesus (04º 04’ 42’’ S e 39º 57’ 08’’ W, 200 m asl), Lagoa do Peixe (03º 56’ 28’’ S e 40º 23’ 23’’ W, 97 m asl), Fazenda Peixes (04º 06’ 03’’ S e 40º 32’ 43’’ W, 114 m asl), Fazenda Natividade (04º 02’ 50’’ S e 40º 29’ 03’’ W, 109 m asl), Fazenda Morro Alto (02º 53’ 42’’ S e 39º 54’ 51’’ W, 16 m asl), Fazenda Araticum (03º 04’ 58’’ S e 40º 09’ 36’’ W, 19 m asl) and Fazenda Experimental da UVA (03º 37' 04'' S 40º 18' 18'' W, 200 m asl).The floristic list consists of 170 species, distributed between 127 genera and 50 families. Twenty-seven Brazilian endemic species were identified, from which 8 are exclusive of the Caatinga biome. The Fabaceae was the most representative family, with the highest number of species (28), followed by Poaceae (17), Malvaceaea (14), Euphorbiaceae (12), Asteraceaea (9), Convolvulaceae and Rubiaceae (9). The dominant life forms were therophytes (34%), phanerophytes (30%) and chamaephytes (18%). Two communities were identified as a result of the classification analysis using the Twinspan.

The flora and vegetation in an area of the Caatinga in Taperuaba, Sobral, Ceará state, Brazil

The Caatinga, covering about 800.000 km2, is the predominant vegetation type of the semi-arid region of Brazil. The Caatinga biome comprises several phytophysiognomies and floristic compositions, with many endemic species, especially in Fabaceae, Cactaceae, Euphorbiaceae, Bignoniaceae e Combretaceae. Despite considerable advances, the Brazilian semi-arid needs more studies and inventories of biodiversity, especially the Ceará state. On the basis of these considerations, the present study aims to identify the flora and vegetation, in order to characterize the phytophysiognomy in an area of the Caatinga, in locality of Taperuaba, municipality of Sobral, Ceará, Brazil. Field work was conducted in March 2015 and 2016 respectively, in three transects. The life-forms were established in accordance of Raunkiaer´s system. The floristic list is composed of 87 species, distributed in 66 genera and 36 families. The flora comprises 22 Brazilian endemic species. The most representative family was Fabaceae with 15 species, followed by Malvaceae (7) Convolvulaceae (6), Euphorbiaceae (5) and Poaceae (5). The biological spectrum had a high proportion of therophytes (29,9%), chamaephytes (29,9%) and phanerophytes (26,4%). In the area were identified two phytophysiognomies: outcrops communities highlighting succulent phanerophytes (Pilosocereus chrysostele (Vaupel) Byles & G.D. Rowley subsp. cearensis P.J. Braun & Esteves and P. gounellei (F.A.C. Weber) Byles & Rowley), chamaephytes (Encholirium spectabile Mart. ex Schult. & Schult. f. and Lepidaploa chalybaea (Mart. ex DC.) H. Rob.) and therophytes (Mitracarpus baturitensis Sucre), mixed with communities including small trees and shrubs on deeper soil, composed of Cereus jamacaru DC., a succulent phanerophyte, and many woody phanerophytes, such as Cordia oncocalyx Allemão, Crateva trapia L., Mimosa caesalpiniifolia Benth., M. tenuiflora (Willd.) Poir., Poincianella bracteosa (Tul.) L.P. Queiroz and P. pyramidalis (Tul.) L.P. Queiroz.