Green mitigation strategy for cultural heritage: bacterial potential for biocide production

Several biosurfactants with antagonistic activity are produced by a variety of microorganisms. Lipopeptides (LPPs) produced by some Bacillus strains, including surfactin, fengycin and iturin are synthesized nonribosomally by mega-peptide synthetase (NRPS) units and they are particularly relevant as antifungal agents. Characterisation, identification and evaluation of the potentials of several bacterial isolates were undertaken in order to establish the production of active lipopeptides against biodeteriogenic fungi from heritage assets. Analysis of the iturin operon revealed four open reading frames (ORFs) with the structural organisation of the peptide synthetases. Therefore, this work adopted a molecular procedure to access antifungal potential of LPP production by Bacillus strains in order to exploit the bioactive compounds synthesis as a green natural approach to be applied in biodegraded cultural heritage context. The results reveal that the bacterial strains with higher antifungal potential exhibit the same morphological and biochemical characteristics, belonging to the genera Bacillus. On the other hand, the higher iturinic genetic expression, for Bacillus sp. 3 and Bacillus sp. 4, is in accordance with the culture antifungal spectra. Accordingly, the adopted methodology combining antifungal screening and molecular data is represent a valuable tool for quick identification of iturin-producing strains, constituting an effective approach for confirming the selection of lipopeptides producer strains.

Toxicological assessment of novel green biocides for cultural heritage.

The damaging of buildings and monuments by biological contamination is a cause of serious concern. Biocides based on chemical toxic compounds have been used to mitigate this problem. However, in the past decade many of the most effective biocides have been banned due to their environmental and health hazards. Therefore, proper remediation actions for microbiologically contaminated historic materials based on environmentally safe solution is of vital importance. Bacillus species are emerging as a promising alternative for built heritage treatment. They produce a great diversity of secondary metabolites with biological activity, well known to possess antagonistic activities against many fungal pathogens. In order to evaluate the antifungal activity of the novel biocides produced in our laboratory by cultures of selected bacterial strains, liquid interaction assays using four biodeteriogenic fungi were achieved, revealing a nearly 100% of inhibitory capacity to fungal proliferation. To confirm their effective safe toxicological properties, in vivo tests using two different biological models were performed. The lyophilized supernatant of the Bacillus culture broth showed no lethality against brine shrimp and also no toxicological effects in Swiss mice through administration of acute dose of 5000 mg/kg by oral gavage. In fact, the bioactive compounds were no lethal at the tested dose unlike Preventol® (commercial biocide) that induced acute toxicity with 10 times minor concentration dose administrated in the same conditions. Therefore, the new bioactive compounds that suppress growth of biodeteriogenic fungi on historical artworks, presenting at the same time no toxicity against other living organisms, constituting an efficient and green safe solution for biodegradation/biodeterioration treatment of Cultural Heritage.

Development and optimisation of protocols for surface cleaning of cultural heritage metals

The conservation and valorisation of cultural heritage is of fundamental importance for our society, since it is witness to the legacies of human societies. In the case of metallic artefacts, because corrosion is a never-ending problem, the correct strategies for their cleaning and preservation must be chosen. Thus, the aim of this project was the development of protocols for cleaning archaeological copper artefacts by laser and plasma cleaning, since they allow the treatment of artefacts in a controlled and selective manner. Additionally, electrochemical characterisation of the artificial patinas was performed in order to obtain information on the protective properties of the corrosion layers. Reference copper samples with different artificial corrosion layers were used to evaluate the tested parameters. Laser cleaning tests resulted in partial removal of the corrosion products, but the lasermaterial interactions resulted in melting of the desired corrosion layers. The main obstacle for this process is that the materials that must be preserved show lower ablation thresholds than the undesired layers, which makes the proper elimination of dangerous corrosion products very difficult without damaging the artefacts. Different protocols should be developed for different patinas, and real artefacts should be characterised previous to any treatment to determine the best course of action. Low pressure hydrogen plasma cleaning treatments were performed on two kinds of patinas. In both cases the corrosion layers were partially removed. The total removal of the undesired corrosion products can probably be achieved by increasing the treatment time or applied power, or increasing the hydrogen pressure. Since the process is non-invasive and does not modify the bulk material, modifying the cleaning parameters is easy. EIS measurements show that, for the artificial patinas, the impedance increases while the patina is growing on the surface and then drops, probably due to diffusion reactions and a slow dissolution of copper. It appears from these results that the dissolution of copper is heavily influenced by diffusion phenomena and the corrosion product film porosity. Both techniques show good results for cleaning, as long as the proper parameters are used. These depend on the nature of the artefact and the corrosion layers that are found on its surface.

Os museus e o património cultural imaterial: Estratégias para o desenvolvimento de boa práticas

omando como referência fundamental o trabalho desenvolvido pela UNESCO em matéria de proteção do Património Cultural Imaterial (PCI), muito particularmente a Convenção para a Salvaguarda do Património Cultural Imaterial (2003), considerou-se oportuno refletir sobre as implicações que este enfoque traz para os museus. São indiscutíveis as repercussões que este instrumento trouxe para o reconhecimento da importância do PCI à escala internacional, motivando um crescendo de iniciativas em tomo da sua salvaguarda. São vários os agentes envolvidos na preservação deste património, no entanto o International Council of Museums (ICOM) reconhece um papel central aos museus nesta matéria. Mas para responder a este repto, os museus terão que repensar as suas estratégias de forma a relacionar-se mais com o PCI, contrariando uma longa tradição profundamente enraizada na cultura material. O presente estudo reflete sobre as possibilidades de actuação dos museus no sentido de dar resposta aos desafios da Convenção 2003, sendo certo que a partir das catividades dos museus é possível encontrar formas de estudar e de dar visibilidade a este património. Em função das especificidades de cada museu, podem ser encontradas estratégias de salvaguarda do PCI, entre as quais se pode incluir o inventário e a documentação (audiovisual, texto, áudio, imagem), a investigação, a divulgação através de exposições e publicações, difusão através da internet, educação não formal, entre outras actividades. Alguns museus começaram já a desenvolver abordagens integradas para a salvaguarda do PCI, cujos exemplos se apresentam. Este tema suscita vários desafios, implicando práticas museológicas inovadoras que possam reflectir o papel dos museus como promotores da diversidade e criatividade cultural. ABSTRACT: Recalling the UNESCO's work towards the protection of Intangible Cultural Heritage (ICH), in particular the Convention for the Safeguarding of the Intangible Cultural Heritage adopted in 2003, I took this opportunity to reflect upon the implications that this recognition brings to museums. The overwhelming success of this document has raised the importance of ICH at international level, motivating a growing number of initiatives towards its safeguard. Accordingly, to the 2003 Convention, there are many agents involved in the preservation of this heritage, yet the International Council of Museums (ICOM) recognizes a central role for museums. Nevertheless, to face this challenge, museums will have to rethink their relationship with ICH in opposition to their deep rooted tradition in material culture. The present study reflects upon the possibilities that museums have to answer the changeling 2003 Convention, recognizing that it’s possible through museum activities to find ways to study and give visibility to ICH. According to each museum specificities, it seems clear that strategies can be engaged in order to promote the safeguard of ICH, including inventory and documentation (audiovisual, audio, text and image), research, promotion through exhibitions, publications, dissemination trough internet and other means, informal education, among other activities. Many museums have already started exploring integrated approaches towards the safeguard of ICH and some of these examples are presented in this study. This theme is challenging, implying innovative museum practices which reflect on museums role towards the promotion of cultural diversity and creativity.

Cultural Values Change in the Rehabilitation of Historic Schools in Portugal

Despite the importance of the preservation of the historic built environment for the benefit of present and future generations, there is a lack of knowledge of the effects of architectural rehabilitation decisions on the cultural significance of historic buildings. Architectural heritage conservation literature has focused almost exclusively on providing principles and guidelines, describing intervention methodologies, and discussing predicted impacts of design on material values. This thesis argues that a focus on the actual effects is needed if the sociocultural sustainability of historic buildings significance is to be achieved. Supported by an extensive literature review and informed by personal insights from the researcher’s everyday practice, an adapted model of the Theory of Change based on Weiss (1995) was designed, providing a tool to evaluate the effects of rehabilitation on cultural significance [ERECS]. Using a selection of six recently rehabilitated historic secondary schools in Portugal (liceus), this research investigated architectural decisions and their effects on the cultural values of this building typology for education, focusing on three objectives, corresponding to three stages of interventions: understanding the existing cultural significance, identifying the design strategies applied and assessing the short-term effects of design decisions on the cultural values. Stressing the role of stakeholders in rehabilitation processes, data were collected from the buildings and architectural projects, the decision makers in the conservation process, and the school community. Although confirming that the evaluation of the effects of architectural decisions on cultural values is a complex task, the findings demonstrate that the historic liceus have historical, architectural and sociocultural values, and whilst strategies did not value social values, material cultural values were generally considered and preserved, contributing to the enhancement of intangible values. The implications of this theory-based and evidence-based research highlight the importance of evaluating actual effects for cultural heritage theory, architectural conservation practice and heritage management policy.

Os moinhos do Rio Almansor no Concelho de Montemor-o-Novo : Um património a conhecer, preservar e valorizar

Esta dissertação tem como tema "Os Moinhos do Rio Almansor no Concelho de Montemor-o-Novo- um património a conhecer, preservar e valorizar", é composta por cinco capítulos, nos quais se pretendeu demonstrar a importância que o património molinológico tem na actualidade, abordar a própria evolução do conceito que abrange hoje os moinhos na vertente cultural, apresentação de alguns estudos e experiências de valorização a nível nacional e internacional deste património, breve descrição da evolução tecnológica tradicional da moagem e análise dos factores geomorfológicos e históricos que estiveram na origem desta actividad em Montemor-o-Novo, o que possibilitou um melhor conhecimento e compreensão do seu desenvolvimento. Um dos principais objectivos foi dar a conhecer os moinhos existentes ao longo das margens do Rio Almansor no Concelho de Montemor, a partir do levantamento patrimonial realizado no terreno, durante o estágio realizado na Câmara Municipal, nomeadamente a sua identificação e caracterização arquitectónica e molinológica. No último capítulo apresenta-se, em resposta ao objectivo principal desta dissertação, uma proposta de valorização dos moinhos, que possa contribuir para a preservação e salvaguarda do pouco, que ainda existe deste património, neste Concelho. A metodologia que se seguiu para a realização desta dissertação assentou em cinco vertentes: em primeiro lugar no levantamento de campo, que foi alvo de pesquisa de localização e identificação dos moinhos no terreno; realização de entrevistas; pesquisa de fontes e de documentação antiga e recolha de bibliografia. Como resultados obtidos pode-se destacar ao longo deste trabalho de dissertação, um conhecimento geral do desenvolvimento da moagem em Portugal, a caracterização e identificação dos moinhos hidráulicos existentes no rio Almansor neste Concelho e a proposta de preservação e valorização deste património molinológico montemorense que poderá servir como exemplo para outras propostas similares, assim como também contribuir para a melhoria de futuras propostas. ABSTRACT; The theme of this dissertation is "The mills of the River Almansor in Montemor­o-Novo County - a cultural heritage to know, preserve and treasure". It's constituted by five chapters, in which we intended to demonstrate the importance that the molinological heritage has nowadays. ln a cultural view it is disseminated on a national and international level by the approaching of the evolution of the concept like it is demonstrated by some studies and experiments here presented, as well as a brief description of the technological evolution of the traditional grinding and analysis of the geomorphologic and historical factors that were in the origin of this activity in Montemor-o-Novo, which allowed a better knowledge and understanding of their development. One of the main goals was to portray the existing mills alongside the river banks of Almansor, in the County of Montemor. lt was made throughout a survey of the patrimonial background on the field, during the training held by the City Hall, including its identification and molinological architectural characterization. ln the last chapter was presented as a response to the main reason of this dissertation, a revival proposal of the mills, which may contribute to the preservation and protection of the few that still remains, in this County. The methodology that was followed for this thesis was based on five aspects: first, the field survey, revealing the research and identification of the location of the mills on the field; interviews, research of sources, old documentation and collection of bibliography.

Le patrimoine culturel universitaire: une méthodologie pour la conservation et la valorisation des biens culturels mobiliers. Le cas de l'Instituto Superior José Antonio Echeverría à la CUJAE - La Havane

Une préoccupation importante pour le patrimoine culturel universitaire vient de se développer en Europe depuis les années 1980. Dans ce riche patrimoine sont inclus les biens mobiliers de valeur historique, scientifico-technique, académique, documentaire, artistique, architecturale et urbaine. Le continent européen a développé durant ces dernières années des actions significatives qui comprennent la création d’organisations, de comités à caractère international, ainsi que la rédaction de recommandations et règlements au niveau européen et institutionnel. Plusieurs universités ont pris des initiatives dans le domaine de la conservation et de la valorisation. À Cuba, il existe un grand intérêt pour le patrimoine culturel de la nation. Malgré la situation économique difficile depuis plusieurs années, Cuba a réussi des bons résultats dans la gestion des centres historiques et des biens patrimoniaux de la nation. Cependant, la thématique liée au patrimoine universitaire est encore peu discutée sur le territoire national, malgré depuis 2010, des actions pour étudier ce patrimoine sont mis en oeuvre grâce aux initiatives des universités de la capitale cubaine. Notre étude de cas, l’Instituto Superior Politécnico José Antonio Echeverría, appartenant au campus CUJAE1, fondé en 1964, a produit un patrimoine culturel universitaire de valeur scientifique, artistique et documentaire qui le distingue des autres universités. Malheureusement, ils existent plusieurs problèmes qui affectent les biens culturels mobiliers de l’ISPJAE et son campus. À partir de la situation actuelle, nous proposons une méthodologie en faveur de la conservation et la valorisation de ses biens, basée sur l’étude des tendances actuelles dans les universités européennes et cubaines étudiées. Les actions proposées favoriseront aussi les biens immobiliers, en tenant compte qu’ils ont un fort lien avec ceux mobiliers; ABSTRACT: An important concern for the university cultural heritage has just developed in Europe since the 1980s. The real asses of historic, scientific-technical, academic, documentary, artistic, architectural values are included in these rich heritages. The European continent developed during the last years significant actions which include the creation of organizations, committees with international character, as well as the writing of recommendations and regulations at the European and institutional level. Several universities took initiatives in the fields of the preservation and the valuation. In Cuba, there is a big interest for the cultural heritage of the nation. In spite of the difficult economic situation for several years, Cuba made a success of good results in the management of the historic centers and the patrimonial asses of the nation. However, the subject related to the university heritage is again little discussed in the national territory in spite of since 2010, several actions to study this heritage are implemented, thanks to the initiatives of the universities of the Cuban capital. Our case of study, Instituto Superior Politécnico José Antonio Echeverría, belonging to the campus CUJAE, established in 1964, produced a university cultural heritage of the scientific, artistic and documentary value, which distinguishes it from other universities. Unfortunately, there are several problems, which affect the real cultural asses of the ISPJAE and its campus. From the current situation, we propose a methodology in favor of the preservation and the valuation of its properties, based on the study of the current trends in the studied European and Cuban universities. The proposed actions will also favor the real property, taking into account that they have a strong link with those movable cultural asses; RIASSUNTO: Una preoccupazione importante per il patrimonio culturale universitario inizia a svilupparsi in Europa dagli anni 1980. In questo ricco patrimonio sono inclusi i beni mobili di valore storico, scientifico-tenico, accademico, documentario, artistico, architettonico e urbano. Il continente europeo ha sviluppato durante questi ultimi anni delle azioni significative che comprendono la creazione di organizazioni, di comitati internazionali, così come la redazione di raccomandazioni e regolamenti a livello europeo e istituzionale. Molte università hanno intrapreso delle iniziative nella conservazione e nella valorizzazione. A Cuba esiste un grande interesse per il patrimonio culturale della nazione. Malgrado la difficile situazione economica da molti anni Cuba ha ottenuto dei buoni risultati nella gestione di centri storici e di beni patrimoniali del Paese. Comunque, la tematica relativa al patrimonio universitario è ancora poco discussa anche se dal 2010 siano messe in opera delle azioni per studiare questo patrimonio, grazie alle iniziative di alcune università della capitale cubana. Il nostro caso di studio, l’Instituto Superior Politécnico José Antonio Echeverría –ISPJAE-, appartenente al campus CUJAE fondato nel 1964, ha prodotto un patrimonio culturale universitario di valore scientifico, artistico e documentale che la distinguono delle altre università. Purtroppo, esistono molti problemi che influenzano i beni culturali mobili dell’ISPJAE e del suo campus. A partire della situazione attuale e facendo parte di questa ricerca, si propone una metodologia a favore della conservazione e della valorizzazione dei loro beni fondata sullo studio delle tendenze attuali in università europee e cubane. Alcune delle azioni proposte sono finalizzate e destinate anche ai beni immobili, tenendo conto che questi hanno un forte rapporto con i beni mobili.

Convento de S. José da Esperança - Évora: proposta de valorização histórico-arquitectónica

A presente dissertação de mestrado visa a apresentação de uma proposta de valorização para o espaço edificado do Convento de S. José da Esperança, da cidade de Évora. Procurámos traçar a história do Convento de S. José ao longo de um período de cerca de 200 anos, apresentando ainda as várias funções do imóvel, desde a sua extinção até à atualidade. Abordámos a questão da instalação física do cenóbio, relacionando-a com as razões da escolha da Ordem Carmelita Descalça. Contextualizámos este especto com as origens da Ordem do Carmo, a reforma desta Ordem, originando o nascimento da Ordem Carmelita Descalça, e a sua entrada em Portugal. Sublinhámos a importância das fundadoras e o seu legado testamentário para a subsistência desta instituição religiosa e como essa subsistência conheceu vicissitudes e contrariedades várias ao longo da vida da comunidade. Procurámos depois entender o espaço conventual edificado, pelo que procedemos a uma análise histórico-arquitetónica do conjunto conventual, nomeadamente a ampliação dos espaços, e depois da extinção e da mudança de propriedade, a adaptação a novas funções e as necessárias intervenções nele ocorridas. Por fim, atendendo à história do Convento, à riqueza patrimonial que ele representa tanto para a sociedade em geral como para os Eborenses em particular, atendendo à sua inserção no Centro Histórico de Évora - CHE, com a classificação de Património Mundial atribuída pela UNESCO em 1986, e atendendo ao vasto quadro legislativo nacional e internacional relativo à proteção e valorização do património cultural, apresentámos a nossa proposta de valorização do Conjunto Conventual, que nos parece, em conformidade com o exposto, a mais apropriada àquele espaço. ABSTRACT: This master's thesis aims to propose a recovery area for the valorization of the building space of Convento de S. José da Esperança, in Évora. We traced the history of the Convent of S. José over a period of about 200 years, by exploring the various functions of the building, since its extinction as religious community until now. We addressed the question of the physical installation the life of the religious community, linking it with the reasons for the choice of the Ordem Carmelita Descalça's to settle there. This aspect was then contextualized with the origins of the Ordem do Carmo, the reform of the Order, resulting in the birth of the Ordem Carmelita Descalça, and its entry into Portugal. We emphasized the importance of the founders and their legacy to the livelihood of the subsistence of this religious institution. Along the history the Ordem experienced several setbacks and problems over the life of the community. Our study led to an understanding of the conventual space as a physical building. For that we undertook an historical and architectonical analysis of the conventual set, namely the enlargement of spaces, and after the extinction and the property change, the adaptation to new functions and the necessary interventions that it suffered. We presented our proposal of historical and architectonical valorization of the convent, taking into account the history of the Convent, as a rich heritage, not only for the city of Évora, but also to the country, as a whole. We took into account the fact that the convent is inside the historical centre of Évora (World Heritage City- UNESCO- since 1986), and it must comply to national and international legislative framework for the protection and enhancement of cultural heritage. Our proposal of recovery of the conventual set is, in line with the above, the most appropriate to that space.

De praça a praça: Praças de toiros portuguesas (1850-1950): contributo para a sua salvaguarda e divulgação

O presente trabalho pretende demonstrar o valor patrimonial das praças de toiros, enquanto manifestações arquitetónicas representativas de uma manifestação cultural e patrimonial herdada - a Tauromaquia. Assim sendo, é nossa intenção criar mecanismos que despertem interesse pelo referido património e que lhe seja reconhecido valor arquitetónico e cultural, sendo que aliada à materialidade do edificado identificámos também aspetos imateriais: costumes, tradições e rituais que, aliados à prática tauromáquica contribuem para a nossa identificação enquanto povo. Através da inventariação do património tauromáquico imóvel existente dentro das balizas cronológicas apontadas procuramos sensibilizar a sociedade para o papel que estes edifícios assumem na história e na cultura das populações, contribuindo simultaneamente para a sua preservação e valorização. O presente trabalho teve como base um vasto leque de leituras alusivas ao tema da tauromaquia que permitiu traçar um percurso de molde a evidenciar a evolução dos preceitos da arte tauromáquica, onde se insere o aparecimento e desenvolvimento das praças de toiros portuguesas. Julgamos que um dos aspetos mais importantes deste trabalho seja o facto da identificação dos espaços e o registo das suas características nos ter permitido conhecer e dar a conhecer a existência de uma tipologia patrimonial que se encontra como que oculta, que para muitos chega mesmo a ser desconhecida ou ignorada, mas que faz parte do nosso património, transcendendo uma dimensão puramente material. ABSTRACT: This paper aims to demonstrate the heritage value of bullrings as architectural manifestations, representing a cultural heritage and legacy - Bullfighting. First of all, the construction of these buildings requires a “will of manifestation" and the recognition of values with which part of the Portuguese people identifies itself. lt is therefore our intention to create mechanisms to awaken interest in this heritage, so that its architectonic and cultural value is recognized. Along with the materiality of the buildings, we have also identified intangible aspects, such as: customs, traditions and rituals, that when allied to bullfighting contribute to our identity as a people. With the inventorying of the bullfighting assets existing within the established timelines, we seek to enlighten society about the role that these buildings assume both in history and culture, as well as contributing to its preservation and enhancement. This research was based on a vast range of readings related to bullfighting which allowed us to outline the path of the evolution of the bullfighting art, which includes the appearing and development of Portuguese bullrings. We believe that one of the most important aspects in this paper is the fact that the identification of the places and the registration of their features has allowed us not only to acknowledge but also to display the existence of a heritage that has been hidden. For many people this is unknown or even ignored, but it's part of our heritage, transcending a purely material dimension.

O lugar da educação patrimonial no currículo escolar

Esta dissertação pretende contribuir no âmbito da gestão e valorização do Património Histórico-Cultural, para o estabelecimento de uma estratégia de valorização e enriquecimento dos currículos escolares portugueses do Ensino Básico, como forma de divulgar, preservar e educar para o Património de um país. Neste contexto emergiu um design de investigação que se afigura pertinente e centrado na prática docente ao fazer um estudo de caso, baseado na opinião recolhida junto dos alunos do 1º ciclo do ensino básico de um agrupamento de escolas sobre a noção que os mesmos têm de Património. A compreensão e valorização da Educação Patrimonial num processo contínuo de descoberta e aprendizagem são tarefas que só a "Escola" pode fazer, formando os indivíduos tomando-os competentes nesta área do conhecimento. Os objectos patrimoniais, monumentos, sítios e centros históricos, ou o património imaterial e natural, são recursos educacionais importantes, permitem a aquisição de competências motivacionais, para qualquer área do currículo ou aproximam áreas aparentemente distantes no processo de ensino e cidadania. ABSTRACT: This thesis aims to contribute in the management and enhancement of Cultural - Heritage for the establishment of a strategy for recovery and enrichment of school curricula elementary Portuguese as a means to educate, preserve and disclose to the heritage of a country. ln this context emerged a research design that seem relevant and focused on teaching to make a case study, based on the feedback gathered from pupils of 1 primary school, a grouping of schools on the notion that their balance sheets. Understanding and appreciation of Heritage Education in a continuous process of discovery and learning are tasks that only the “School” can do, forming the individuals making them competent in this field of knowledge. Heritage objects, monuments, sites and historical centres, or intangible and natural heritage, are important educational resources, enable the motivational skills acquisition, to any area of the curriculum or assemble seemingly distant areas in education and citizenship.

Repensar=Reutilizar. o abandono como oportunidade. Fábrica Robinson: o instante na construção de um tempo provisório

Esta investigação procura mostrar/problematizar programas que questionem a reutilização de lugares, através de uma proposta de projeto provisório de arquitetura, no panorama do Património Industrial, num processo de revitalização. O intuito é procurar modelos/ações que possam responder às transformações, partindo do problema do abandono, dos espaços pós-industriais. O objetivo principal consiste em criar um processo de aproximação à problemática da reutilização cujo processo de elaboração de projeto encontra nas suas formas de produção e operação um discurso que faz a síntese entre prática artística, posição curatorial e arquitetura, na elaboração de um programa para um lugar revitalizando-o. O objeto deste estudo faz parte de uma investigação de pesquisa por projeto e encontra lugar no conjunto arquitetónico da Fábrica Robinson, em Portalegre, cuja apropriação inicial de parte do Convento de S. Francisco dá hoje lugar ao Espaço Robinson. O reconhecimento deste conjunto, como lugar de encontro entre um passado recente e um futuro pendente, marcou a proposta projetual. Proposta de projeto temporário cujo conceito estabelece a viabilidade de apropriação como forma de atuação provisória, subjacente ao ato de reutilizar, criando novos programas de ocupação, promovendo novas ideias e definições de património e assim devolvendo o significado às estruturas industriais vazias. O que preservar e como conservar pode demarcar a importância de salvaguarda deste património e a previsão da sua transmissão para o futuro, fixada pela ideia de preservação. A Fábrica Robinson e a revitalização urbana deste espaço configura, hoje, uma nova oportunidade para estes conjuntos arquitetónicos, e a importância da sua salvaguarda, na valorização do Património Industrial. O trabalho pretende demonstrar a ordem de relação entre programa e lugar no debate interdisciplinar e a hipótese de concretização projetual que preencha um vazio temporalmente suspenso; ABSTRACT: Rethinking = Reuse: The abandonment as opportunity Robinson Factory: The instant in the construction of a provisory time This research aims to show architectural programs that question the reuse of places in the context of industrial heritage, through an architectural design, in a revitalization process. The aim is to look to models / actions that, starting with the abandonment problem, may answer to the post-industrial spaces transformations. The main goal is to create an approximation process to the problem of reuse, whose project design process finds in its production and operations forms a symbiosis between artistic practice, curatioral approach and architecture, in the development of a program to revitalize a place. The object of this study is part of a research project / research by design concerning the architectural complex Fábrica Robinson located in Portalegre, whose original appropriation of part of Convento de S. Francisco is today occupied by Espaço Robinson. The acknowledgment of this complex as a space placed in-between a recent past and a pending future marked the design proposed. A temporary design proposal whose concept establishes the validity of the temporary action, underlying to the act of reuse, creating new occupation programs, promoting new ideas and heritage definitions and thus given back meaning to the empry industrial structures. What to preserve and how to save may mark the importance of safeguarding this heritage and the prediction of its transmission to the future, set by the idea of preservation. The Fábrica Robinson and the urban regeneration of this area sets itself as a new opportunity to safeguard these architectural ensembles and enhance the value of the industrial heritage. This study intend to show the relationship between the program and a vacant place, through a temporary proposal, supported by the interdisciplinary debate and the hypothesis of design implementation that completes a void temporarily suspended.

Novos desenvolvimentos para a deteção de leveduras e bactérias presentes em argamassas

The development of simple and rapid new approaches for analysing microbial communities colonising Cultural Heritage materials is pivotal for its safeguard. Fluorescence in situ hybridisation technique using ribosomal RNA directed probes (RNA-FISH) has demonstrated a great potential for this purpose. A protocol for analysing filamentous fungi in mortars has been already developed in previous studies. In this work this protocol has been adapted for detecting bacteria and yeasts. Good results have been obtained for the analysis of suspensions of isolates. In this way, the optimized protocol was applied in microsamples from synthetic mortar artificially inoculated with yeast and bacterial isolates. Promising results have been obtained for the ex situ analysis of yeast and bacteria thriving in mortar microsamples.

Reconstituição histórica virtual do retábulo-mor da Igreja do Espírito Santo de Évora: aplicação ao Património da infografia web-based

As a part of a multidisciplinary and integrated research, including conservation sciences and history, a proposal is presented for the historical reconstitution and the virtual restoration of the mannerist altarpiece of the main altar at the Espírito Santo Church, in Évora. The collected data is abundant and the scientific information, because of its technicality, is less prone to be easily understood by the general public, thus becoming less accessible. Web-based infographics are explored as privileged forms of disseminating results and raising awareness to Cultural Heritage. The project materializes as an Internet platform where data and a reconstitution proposal are shared in a visual and interactive way. In addition to the digital virtual reconstitution (2D), some tridimensional models (3D) are presented of various elements of the altarpiece, obtained using methods of computer graphics and digital photogrammetry

Darkening on Lead based pigments: microbiological contribution

The biodeterioration/biodegradation process is an important issue for the conservation of cultural heritage that needs urgent answers to their rehabilitation. In this way, the role of microorganisms in surfaces alteration was exploited. This work revealed a strong relationship between the microbiological proliferation and the damaged areas, evidencing the important role of the microorganisms in mural paintings alteration process. The oxidation of lead-based pigments noticeably contributes to the pigments alteration, and seems to be correlated with the presence of biodeteriorative microorganisms. The study of the mechanisms underlying the microbiological attack of mural paintings has been explored to understand as much as possible the proliferative ability and biodeteriorative capacity of the microorganisms, related to darkening on lead-based pigments

Combined Use of NMR, LC-ESIMS and Antifungal Tests for Rapid Detection of Bioactive Lipopeptides Produced by Bacillus

The science and technology interact with the art in several ways. Biotechnological coupled with analytical approaches can play an important role in protecting and preserving cultural heritage for future generations. Many microorganisms influenced by environmental conditions are the main responsible for biological contamination in built heritage. Biocides based on chemical compounds have been used to mitigate this problem. Thus, it is vitally important to develop proper remediation actions based on environmentally innocuous alternative. Bacillus specie is emerging as an optimistic alternative for built heritage treatment due to their capacity to produce secondary metabolites with antagonistic activities against many fungal pathogens. Therefore, the intent of this work was to access a rapid evaluation of antifungal potential of bioactive metabolites produced by Bacillus strains and simultaneously their characterization using spectroscopic (NMR) and chromatographic techniques (LCESI- MS). The high antifungal activity obtained for Bacillus sp. active compounds produced in this study confirms the great potential to suppress biodeteriogenic fungi growth on historical artworks. Additionally, the proposed methodology allowed to access bioactive metabolites produced without need of the laborious total previous isolation and could be used as a viable alternative to be employed for screening and production of new green biocides.