“Biomarkers in a One Health perspective: current knowledge of their ability to merge human and animal health

Biomarkers are nowadays essential tools to be one step ahead for fighting disease, enabling an enhanced focus on disease prevention and on the probability of its occurrence. Research in a multidisciplinary approach has been an important step towards the repeated discovery of new biomarkers. Biomarkers are defined as biochemical measurable indicators of the presence of disease or as indicators for monitoring disease progression. Currently, biomarkers have been used in several domains such as oncology, neurology, cardiovascular, inflammatory and respiratory disease, and several endocrinopathies. Bridging biomarkers in a One Health perspective has been proven useful in almost all of these domains. In oncology, humans and animals are found to be subject to the same environmental and genetic predisposing factors: examples include the existence of mutations in BR-CA1 gene predisposing to breast cancer, both in human and dogs, with increased prevalence in certain dog breeds and human ethnic groups. Also, breast feeding frequency and duration has been related to a decreased risk of breast cancer in women and bitches. When it comes to infectious diseases, this parallelism is prone to be even more important, for as much as 75% of all emerging diseases are believed to be zoonotic. Examples of successful use of biomarkers have been found in several zoonotic diseases such as Ebola, dengue, leptospirosis or West Nile virus infections. Acute Phase Proteins (APPs) have been used for quite some time as biomarkers of inflammatory conditions. These have been used in human health but also in the veterinary field such as in mastitis evaluation and PRRS (porcine respiratory and reproductive syndrome) diagnosis. Advantages rely on the fact that these biomarkers can be much easier to assess than other conventional disease diagnostic approaches (example: measured in easy to collect saliva samples). Another domain in which biomarkers have been essential is food safety: the possibility to measure exposure to chemical contaminants or other biohazards present in the food chain, which are sometimes analytical challenges due to their low bioavailability in body fluids, is nowadays a major breakthrough. Finally, biomarkers are considered the key to provide more personalized therapies, with more efficient outcomes and fewer side effects. This approach is expected to be the correct path to follow also in veterinary medicine, in the near future.

O conhecimento profissional do professor e as investigações matemáticas na sala de aula: Um estudo nos 1º e 2º ciclos do ensino básico

Este estudo tem como objetivo contribuir para a compreensão do conhecimento profissional do professor de Matemática envolvido no desenvolvimento de tarefas de investigação na sala de aula, focando-se em quatro questões orientadoras: (i) como é que os professores colocam em prática as tarefas de investigação, considerando a planificação e condução das aulas? (ii) que dificuldades sentem os professores no desenvolvimento de tarefas de investigação na sala de aula? (iii) como é que o conhecimento didático dos professores influencia o desenvolvimento de tarefas de investigação? (iv) como é que o conhecimento didático dos professores é influenciado pelo desenvolvimento de tarefas de investigação? A fundamentação teórica assenta sobre as duas grandes temáticas do estudo: o conhecimento profissional do professor, sendo discutida a sua natureza, estrutura e conteúdo, e as tarefas de investigação, das quais se aprofunda a natureza e a utilização em sala de aula. Apresenta-se ainda investigação empírica referente ao conhecimento profissional do professor envolvido no desenvolvimento de tarefas de investigação. O estudo assumiu uma abordagem interpretativa, concretizando-se através dois estudos de caso, um de uma professora de 1. ° Ciclo e outro de um professor de Matemática do 2. ° Ciclo do Ensino Básico. Estes professores trabalharam em colaboração com a investigadora sobre tarefas de investigação, que até aí não conheciam, planificando, lecionado e refletindo acerca de um conjunto de aulas com tarefas desta natureza. Foram recolhidos dados durante as sessões de trabalho referidas, bem como nas aulas e ainda em entrevistas realizadas a cada um dos professores. Uma leitura transversal dos casos permite retirar algumas conclusões. A possibilidade de enquadramento nos conteúdos programáticos parece ser o principal critério de seleção das tarefas por parte dos professores. Na planificação, é a inventariação dos conteúdos que a tarefa mobiliza a principal prioridade, na busca da redução do grau de incerteza relacionado com as eventuais reações dos alunos. Quanto à condução de tarefas de investigação, a fase de introdução foi a mais breve, tendo servido o objetivo de conduzir à compreensão do propósito da tarefa por parte dos alunos e também reforçar alguns aspetos metodológicos do trabalho investigativo. A fase de desenvolvimento da tarefa foi aquela a que o professor de 2. ° Ciclo atribuiu maior importância, embora também valorizada pela professora de 1. ° Ciclo. Aqui, os professores consideram que o seu papel é acompanhar e orientar o trabalho dos alunos, ajudando a ultrapassar possíveis obstáculos, mantendo uma atitude questionadora. Quanto à fase de discussão, a grande preocupação dos professores é garantir a compreensão das ideias apresentadas pelos alunos. No entanto, existem duas estratégias distintas: enquanto a professora de 1. ° Ciclo, que muito valorizou esta fase, estimula a interação aluno-aluno, atribuindo-lhes a responsabilidade de validar/rejeitar as conjeturas apresentadas, o professor de 2. ° Ciclo centra em si toda esta fase, assumindo a responsabilidade de corrigir as ideias apresentadas. O desenvolvimento de tarefas de investigação é influenciado pelo conhecimento didático, revelando-se determinantes o conhecimento dos alunos e da forma como aprendem e o conhecimento do processo instrucional. O desenvolvimento de tarefas de investigação influencia várias componentes do conhecimento didático do professor, permitindo-lhe fortalecer o seu conhecimento matemático, desenvolver um conhecimento mais completo dos alunos e da forma como aprendem. Por último, a prática letiva com tarefas de investigação fornece ao professor dados importantes de como planificar e conduzir tarefas de natureza aberta, de forma a tirar o máximo partido das suas potencialidades. ABSTRACT: This study’s aim is to contribute to the understanding of the professional knowledge of the Maths teacher, involved in the development of investigative tasks in the classroom, focusing four guiding questions: (i) how do teachers give practical way to the investigative tasks, having in mind the lesson plans and lesson execution? (ii) Which difficulties do the teachers face when developing the investigative tasks in the classroom? (iii) How does the teachers' didactical knowledge influence the development of the investigative tasks? (iv) How is the teachers' didactical knowledge influenced by the development of the investigative tasks? The theorical framework is based on two broad themes of the study: teacher's professional knowledge (discussing its nature, structure and contents) and the investigative tasks (whose nature and use in the classroom is deepened). Besides, empirical research in what concerns to the professional knowledge of the teacher involved in the development of investigative tasks is also added. The study assumed an interpretative approach, materialised through two case studies, one of a primary school teacher, the other of a preparatory school Maths teacher. The teachers worked in cooperation with the researcher on the investigative tasks, which, until then, they weren't familiar with, planning, teaching and thinking about a set of lessons with tasks of this kind. Data were collected during the work meetings, as well as in the lessons and also in interviews to each one of the teachers. A transversal appreciation of the cases allows us to draw some conclusions. The possibility of integrating the programmatic contents seems to be the main criteria for the selection of the tasks by the teachers. ln the planning, the main priority of the task is the inventory of contents, aiming to reduce the level of uncertainty in what concerns to eventual reactions of the students. ln what concerns to the conduction of the investigative tasks, the introduction phase was the shortest, fulfilling the aim of leading to the understanding of the proposal of the task by the students and also to reinforce some methodological aspects of the investigative work. The task's development phase was the one the preparatory school teacher dedicated more importance, though it was also valued by the primary school teacher. ln this aspect, the teachers consider that their role is to follow and lead the students’ work, helping them to overtake eventual obstacles, keeping the questioning attitude. ln what concerns the discussion phase, the big concern of the teachers is to reassure the understanding of the ideas presented by the students. However, there are two different strategies: while the primary school teacher, who really valued this phase, stimulates the student-student interaction, giving them the responsibility of validating/rejecting the presented conjectures; the preparatory school teacher centers around himself all this phase, assuming the responsibilities of correcting the presented ideas. The development of the investigative tasks is influenced by the didactical knowledge, and it is determinant the knowledge about the students and about the way they learn and the instructional knowledge. The development of the investigative tasks influences several components of the didactical knowledge of the teacher, allowing him/her to reinforce his mathematical knowledge, developing a more complete knowledge about the students and the way they learn. Finally, the lessons execution with investigative tasks provides the teacher with important data about how to plan and conduct open nature tasks, in order to take the biggest advantage of their potential.