O sistema de informação do tribunal colectivo dos géneros alimentícios: Estudo orgânico-funcional, produção e tramitação documental

Este trabalho é um estudo sobre o sistema de informação Tribunal Colectivo dos Géneros Alimentícios, como nasceu e como se desenvolveu, através do estudo orgânico-funcional, análise da produção e tramitação documental, desde a sua criação (1930) até à sua extinção (1976), através da aplicação do método de investigação quadripolar. Este método vai desenrolar-se mediante três actividades, cujo exercício se integra numa abordagem sistémica: a) Estudo da natureza da instituição a partir da análise dos seus fins, dos seus órgãos, das suas funções e da sua prática administrativa, tendo em conta tanto a evolução destes elementos ao longo do tempo como as relações que se estabelecem entre eles, através da análise de leis orgânicas e outros diplomas legais. b) Identificação e delimitação das séries documentais a partir da análise dos modos de produção documental, as características externas e internas destes tipos de documentos e as operações de conservação, descrição e organização arquivística. c) Apresentação do quadro de classificação: estrutura hierárquica e lógica que reflicta as funções deste sistema de informação. Pretende-se dar resposta às seguintes questões: 1- como classificar arquivisticamente a documentação acumulada no Arquivo Tribunal Colectivo dos Géneros Alimentícios? 2- Classificar arquivisticamente os processos de infracções/crimes do Tribunal de Recurso da Intendência-Geral da Segurança Pública e os processos de infracções/crimes até 1936 no sistema Tribunal Colectivo dos Géneros Alimentícios. 3- A produção e o circuito documental do Tribunal Colectivo dos Géneros Alimentícios são semelhantes à produção documental dos tribunais de jurisdição ordinária? 4- Quais a(s) especificidade(s) a nível processual e de tipologias documentais. ABSTRACT: This thesis is a study on the information system of the Tribunal Colectivo dos Géneros Alimentícios: how it started and how it was developed, and we do so proceding with the study of the organizational system, of the channeling of the proceedings and documents, since its creation (1930) until its ending (1976), using the quadripolar research method. This method will develop into three different steps, following a systemic approach and using the Quadripolar Method: a) A study of the nature of the institution supported by the analysis of its purposes, its organs, its functions and its administrative practices, bearing in mind the evolution of these elements throughout time and the connections that are established between them, with the support of some organic laws and other legal diplomas. b) Identification and caracterization of the document series; the method that it will be used will include an analysis of the process of document production, and also of both the internal and external characteristics of that type of documents and the archival conservation, description and organization. c) Demonstration of the classification board: hierarchic structure and the logic that is behind the institution of this documental base. This work intends to answer the following questions: 1. How to classify archival documentation accumulated in the Tribunal Colectivo dos Géneros Alimentícios? 2. How to classify the offense/crimes processes at the Tribunal de Recurso at Intendência-Geral da Segurança Pública and the offense/crimes processes until 1936 at Tribunal Colectivo dos Géneros Alimentícios? 3. Are the circuit and the document production of the Tribunal Colectivo dos Géneros Alimentícios similar to the production of documents in regular jurisdiction courts? 4. What are the specific level procedural and documentary types?

A rapid staining-assisted wood sampling method for PCR-based detection of pine wood nematode Bursaphelenchus xylophilus in Pinus massoniana wood tissue

For reasons of unequal distribution of more than one nematode species in wood, and limited availability of wood samples required for the PCR-based method for detecting pinewood nematodes in wood tissue of Pinus massoniana, a rapid staining-assisted wood sampling method aiding PCR-based detection of the pine wood nematode Bursaphelenchus xylophilus (Bx) in small wood samples of P. massoniana was developed in this study. This comprised a series of new techniques: sampling, mass estimations of nematodes using staining techniques, and lowest limit Bx nematode mass determination for PCR detection. The procedure was undertaken on three adjoining 5-mg wood cross-sections, of 0.5 · 0.5 · 0.015 cm dimension, that were cut from a wood sample of 0.5 · 0.5 · 0.5 cm initially, then the larger wood sample was stained by acid fuchsin, from which two 5-mg wood cross-sections (that adjoined the three 5-mg wood cross-sections, mentioned above) were cut. Nematode-staining-spots (NSSs) in each of the two stained sections were counted under a microscope at 100· magnification. If there were eight or more NSSs present, the adjoining three sections were used for PCR assays. The B. xylophilus – specific amplicon of 403 bp (DQ855275) was generated by PCR assay from 100.00% of 5-mg wood cross-sections that contained more than eight Bx NSSs by the PCR assay. The entire sampling procedure took only 10 min indicating that it is suitable for the fast estimation of nematode numbers in the wood of P. massonina as the prelimary sample selections for other more expensive Bx-detection methods such as PCR assay.