Living on the intertidal/subtidal edge: ecology of the stalked barnacle Pollicipes Pollicipes and of the sea urchin Paracentrotus lividus

This thesis contributes to the knowledge of temperate rocky shore ecology, with direct implications in the management and conservation of two important local marine resources that inhabit the very edge of subtidal and intertidal habitats on wave-swept rocky shores: the sessile filter feeding stalked barnacle Pollicipes pollicipes and the mobile keystone herbivore sea urchin Paracentrotus lividus. Research was conducted along gradients of wave exposure on rocky shores of Southern Europe. The abundance of P. lividus was four times higher in SW Portugal than in NW Italy. Most of the variation in urchin abundance occurred at small spatial scales, probably shaped by habitat complexity. In SW Portugal, sea urchin attachment forces while in burrows were measured and related to burrow shape, urchin size and habitat. Burrowing behaviour enhances sea urchin attachment force and might be an adaptive response to hydrodynamic stress. Abundance of P. pollicipes in SW Portugal is highly and positively related to wave exposure at local and regional scales. Predation and recruitment processes seem to be important drivers of these abundance patterns. A distribution model of P. pollicipes abundance in relation to wave exposure was developed for the SW coast of Portugal and might be used for improvement of its management and conservation. Growth of P. pollicipes was studied by applying a novel method using fluorescent calcein for marking and to estimate growth. Growth rate decreased with barnacle size and was highly variable amongst individuals, particularly in smaller barnacles. No effect of shore level on barnacle growth was detected. An assessment of the state of the fishery, conservation and management of the stalked barnacle in different regions of continental Portugal was made, highlighting an overall negative tendency of this state and recommending a change into a co-management system; Resumo: Viver no limite intertidal / subtidal: ecologia do percebe Pollicipes pollicipes e do ouriço-do-mar Paracentrotus lividus Esta tese contribui para o conhecimento da ecologia do litoral rochoso e tem implicações diretas na gestão e conservação de dois recursos marinhos locais que habitam o interface subtidal/intertidal de costas rochosas sujeitas a agitação marinha: o percebe Pollicipes pollicipes, animal séssil e filtrador e o ouriço-do-mar Paracentrotus lividus, animal móvel e herbívoro. Foram realizados vários estudos ao longo de gradientes de hidrodinamismo em costas rochosas do sul da Europa. A abundância de P. lividus foi quatro vezes superior no sudoeste de Portugal relativamente ao noroeste de Itália. Grande parte da variação na abundância de P. lividus ocorreu a pequenas escalas espaciais, provavelmente influenciada pela complexidade do habitat. A força com que o ouriço-do-mar se fixa ao substrato foi medida no terreno no sudoeste de Portugal, tendo esta sido relacionada com a forma da depressão que ocupa, o tamanho individual e o habitat. O comportamento escavador desta espécie aumenta a sua força de fixação ao substrato e poderá ser uma resposta adaptativa ao hidrodinamismo.. A abundância de P. pollicipes na costa sudoeste de Portugal, a diferentes escalas espaciais, está relacionada de forma positiva com a agitação marinha, e é influenciada pela predação e pelo recrutamento desta espécie. Foi desenvolvido um modelo de distribuição e abundância de P. pollicipes para esta costa baseado na relação com a agitação marinha, cujos resultados podem ser usados para melhorar a gestão e conservação deste recurso. Um novo método com recurso a calceina fluorescente foi desenvolvido para marcar percebes e estudar o seu crescimento. A taxa de crescimento diminuiu com o tamanho do animal, sendo altamente variável entre indivíduos, sobretudos nos de menores dimensões. O estado da apanha, conservação e gestão do percebe em diferentes regiões de Portugal Continental apresentou uma tendência global negativa, e recomenda-se uma alteração para um sistema de cogestão deste recurso.

Performance of a vertical slot fish pass for the sea lamprey Petromyzon marinus L. and habitat recolonization.

In 2011, a vertical-slot fish pass was built at the Coimbra Açude-Ponte dam (Mondego River, Portugal), approximately 45 km upstream from the river mouth. The performance of this infrastructure for sea lamprey passage was evaluated between 2011 and 2015 using several complementary methodologies, namely radio telemetry [conventional and electromyogram (EMG)], passive integrated transponder (PIT) telemetry and electrofishing surveys. During the study period, the electrofishing revealed a 29-fold increase in the abundance of larval sea lamprey upstream of the fish pass. Of the 20 radio-tagged individuals released downstream from the dam, 33% managed to find and successfully surpass the obstacle in less than 2 weeks, reaching the spawning areas located in the upstream stretch of the main river and in one important tributary. Fish pass efficiency was assessed with a PIT antenna installed in the last upstream pool and revealed a 31% efficiency, with differences between and within migratory seasons. Time of day and river flow significantly influenced the attraction efficiency of the fish pass, with lampreys negotiating it mainly during the night period and when discharge was below 50m3 s_1. Sea lampreys tagged with EMG transmitters took 3 h to negotiate the fish pass, during which high muscular effort was only registered during passage, or passage attempts, of the vertical slots. The use of complementary methodologies provided a comprehensive passage evaluation for sea lamprey, a species for which there is a considerable paucity of valuable data concerning behavioural, physiological and environmental influences on obstacle negotiation.