Satellite DNA as a target for TaqMan real-time PCR detection of the pinewood nematode, Bursaphelenchus xylophilus

The pinewood nematode (PWN), Bursaphelenchus xylophilus , is a major pathogen of conifers, which impacts on forest health, natural ecosystem stability and international trade. As a consequence, it has been listed as a quarantine organism in Europe. A real-time PCR approach based on TaqMan chemistry was developed to detect this organism. Specific probe and primers were designed based on the sequence of the Msp I satellite DNA family previously characterized in the genome of the nematode. The method proved to be specific in tests with target DNA from PWN isolates from worldwide origin. From a practical point of view, detection limit was 1 pg of target DNA or one individual nematode. In addition, PWN genomic DNA or single individuals were positively detected in mixed samples in which B. xylophilius was associated with the closely related non-pathogenic species B. mucronatus , up to the limit of 0.01% or 1% of the mixture, respectively. The real-time PCR assay was also used in conjunction with a simple DNA extraction method to detect PWN directly in artificially infested wood samples. These results demonstrate the potential of this assay to provide rapid, accurate and sensitive molecular identification of the PWN in relation to pest risk assessment in the field and quarantine regulation.

Histoire et projet dans la valorisation du patrimoine de la soie : Le Filatoio di Caraglio (Coni. Italie) et le Real Filatório de Chacim (Trás-os-Montes, Portugal)

O objetivo deste trabalho é explorar a temática da valorização do patrimônio arquitetónico, herdado pela indústria da seda, baseando-se na história das técnicas, na gestão e na valorização do patrimônio industrial. É a partir do estudo de duas fábricas de seda na Europa: o Filatoio di Caraglio em Itália (data de construção: 1676-1678) e o Real Filatório de Chacim em Portugal (data de construção: 1788) que serão discutidas as estratégias encontradas para a conservação/restauração dos dois casos em questão, com base em suas histórias e contextos atuais. O Filatoio di Caraglio é uma das fábricas de seda mais antigas da Europa tendo como técnica o moinho alia piemontese publicado na Encyclopédie como uma das melhores invenções. No caso do Real Filatório de Chacim, constata-se que a introdução deste moinho por meio de técnicos italianos constituiu uma transferência tecnológica entre os dois países. RÉSUMÉ: L'objective de ce travail est d'explorer la thématique de la mise en valeur du patrimoine architectural hérité de industrie de la soie en se basent sur l'histoire des techniques, la gestion et la valorisation du patrimoine industriei. C'est à partir de l'étude de deux fabriques à soie en Europe: le Filatoio di Caraglio en Italie (date de construction: 1676-1678) et le Real Filatório de Chacim au Portugal (date de construction: 1788) que sont discutées les stratégies employées pour la conservation/restauration de ces deux cas basées sur leur histoire et sur les contextes actuels. Le Filatoio di Caraglio est une des soieries plus anciennes de l'Europe et était caractérisée par une technique du moulin alla piemontese publié dans l'Encyclopédie come une des meilleures inventions. Dans le cas du Real Filatório de Chacim, on constate que l'introduction de ce type de moulin par des techniciens italiens a constitué un transfert technique entre les deux pays. ABSTRACT: The purpose of this research is to examine the enhancement of architectural heritage inherited from the silk industry, focusing on technical history, and the management and valorization of industrial heritage. Conservation/restoration strategies are discussed by analyzing solutions proposed in two European silk mills: The Filatoio di Caraglio, in Italy (date of construction: 1676-1678) and the Real Filatório de Chacim, in Portugal (date of construction: 1788), taking into consideration their histories and current context. While the former is one of the oldest European silk mills, employing the alla piemontese technique, which was praised by the Encyclopédie, the latter was set up by ltalian technicians, establishing a technology transfer between the two countries.