Distinguishing the distributions of two cryptic frogs (Anura: Discoglossidae) using molecular data and environmental modeling

Currently, the identification of two cryptic Iberian amphibians, Discoglossus galganoi Capula, Nascetti, Lanza, Bullini and Crespo, 1985 and Discoglossus jeanneae Busack, 1986, relies on molecular characterization. To provide a means to discern the distributions of these species, we used 385-base-pair sequences of the cytochrome b gene to identify 54 Spanish populations of Discoglossus. These data and a series of environmental variables were used to build up a logistic regression model capable of probabilistically designating a specimen of Discoglossus found in any Universal Transverse Mercator (UTM) grid cell of 10 km × 10 km to one of the two species. Western longitudes, wide river basins, and semipermeable (mainly siliceous) and sandstone substrates favored the presence of D. galganoi, while eastern longitudes, mountainous areas, severe floodings, and impermeable (mainly clay) or basic (limestone and gypsum) substrates favored D. jeanneae. Fifteen percent of the UTM cells were predicted to be shared by both species, whereas 51% were clearly in favor of D. galganoi and 34% were in favor of D. jeanneae, considering odds of 4:1. These results suggest that these two species have parapatric distributions and allow for preliminary identification of potential secondary contact areas. The method applied here can be generalized and used for other geographic problems posed by cryptic species.

Um café pela natureza, qual a melhor opção

O café é originário das florestas tropicais da Etiópia e do Sudão, crescendo sobre uma canópia diversa. Devido ao facto de haver uma tendência para o desaparecimento do sombreamento tradicional e consequente perda de biodiversidade, os sistemas de certificação oferecem a oportunidade de aproximar objectivos económicos, sociais e ambientais de modo a beneficiar os agricultores. Este estudo tem como objectivo dar a conhecer a todos os intervenientes da cadeia de custódia, principalmente aos consumidores, qual a certificação que podem optar por se a sua principal preocupação quando tomam um café for a natureza. Caracterizou-se o valor ecológico do sistema de produção sustentável e do sistema convencional. Analisaram-se e comparam-se os diferentes critérios ambientais dos sistemas de certificação, através da construção de uma matriz. Os resultados sugerem que a certificação que melhor responde ao pretendido é a Rainforest Alliance, no entanto, a dupla ou tripla certificação parecem ser também boas opções. ABSTRACT; Coffee originates in Ethiopia and Sudan’s ram forests, growing above a diverse canephora. Due to the fact that there is a tendency to the vanishing of traditional shadowing and consequent loss of biodiversity, certification schemes offer the opportunity of bringing together economical, social and environmental goals allowing benefits to farmers. This study aims to bring cognizance to every intervenient of the custody chain, mainly to consumers, which certification scheme to choose if their major concern when drinking a coffee is nature. The ecological value of sustainable production system has been characterized as well as the conventional system. Different environmental criteria of certification schemes have been analysed and compared trough the construction of an evaluation template. Results suggest that the certification that better suits the desired goal is the Rainforest Alliance, but double or triple certification also appear as options that might be followed.