Multicentric genome-wide association study for primary spontaneous pneumothorax

Despite elevated incidence and recurrence rates for Primary Spontaneous Pneumothorax (PSP), little is known about its etiology, and the genetics of idiopathic PSP remains unexplored. To identify genetic variants contributing to sporadic PSP risk, we conducted the first PSP genome-wide association study. Two replicate pools of 92 Portuguese PSP cases and of 129 age- and sex-matched controls were allelotyped in triplicate on the Affymetrix Human SNP Array 6.0 arrays. Markers passing quality control were ranked by relative allele score difference between cases and controls (|RASdiff|), by a novel cluster method and by a combined Z-test. 101 single nucleotide polymorphisms (SNPs) were selected using these three approaches for technical validation by individual genotyping in the discovery dataset. 87 out of 94 successfully tested SNPs were nominally associated in the discovery dataset. Replication of the 87 technically validated SNPs was then carried out in an independent replication dataset of 100 Portuguese cases and 425 controls. The intergenic rs4733649 SNP in chromosome 8 (between LINC00824 and LINC00977) was associated with PSP in the discovery (P = 4.07E-03, ORC[95% CI] = 1.88[1.22–2.89]), replication (P = 1.50E-02, ORC[95% CI] = 1.50[1.08–2.09]) and combined datasets (P = 8.61E-05, ORC[95% CI] = 1.65[1.29–2.13]). This study identified for the first time one genetic risk factor for sporadic PSP, but future studies are warranted to further confirm this finding in other populations and uncover its functional role in PSP pathogenesis.

A gestão de recursos humanos numa organização de saúde

A Gestão de Recursos Humanos é uma área que nos últimos anos se tem vindo a destacar, possibilitando uma empresa a prosperar ou não. Assim, desde que as estratégias organizacionais sofreram uma redefinição, os recursos humanos das empresas são vistos como uma fonte de vantagem competitiva. Este Trabalho Final de Mestrado faz uma descrição das atividades desenvolvidas, segundo o plano elaborado, no estágio académico durante 6 meses, com vista ao desenvolvimento pessoal, mas acima de tudo profissional e de experiência na área dos Recursos Humanos, através do qual se visa a aquisição e o desenvolvimento de conhecimentos e aptidões de prática organizacional numa organização de saúde. A metodologia utilizada foi do tipo observação participante e a análise da teoria para melhor entender e agir diligentemente nas tarefas em que se participa. O trabalho final seguirá a forma de um Relatório de Estágio e consequentes críticas que possam ter surgido durante o mesmo; Abstract: The Human Resources Management in a Health Organization The Human Resource Management is an area that in recent years has emphasized, enabling a company to prosper or not. So, since the organizational strategies suffered a redefinition, human resources companies are seen as a source of competitive advantage. This Master Final Work is a description of activities, according to the plan drawn up in the academic internship for 6 months with a view to personal development, but above all professional and experience in the field of Human Resources, through which it aims the acquisition and development of knowledge and skills of organizational practice in a healthcare organization. The methodology used was the type participant observation and analysis of the theory to better understand and act diligently on the tasks in which they participate. The final work will follow the form of an internship report and subsequent criticism that may have arisen in the meantime.

Thermoregulatory response in hair sheep and shorn wool sheep

This study’s main goal was to evaluate the thermoregulatory responses velocity through the variation of rectal temperature (RT), related to the thermolytic pathways, respiratory rate (RR) and sweating rate (SR) among different sheep breeds. Ninety female sheep, eighteen of each breed: Santa Ines and Morada Nova (Brazilian hair breeds), Texel, Suffolk and Ile de France (wool breeds) were challenged during three non-consecutive summer days (22◦42′S, 47◦18′W, and 570m of altitude, maximum air temperature of 33.5◦C, average relative humidity of 52±6.9%). The physiological variables were registered at 0800h (T1), 1300 h (T2: after 2 h of shade rest), 1400 h (T3) (after one hour of sun exposure) and in the shade at 1415 h (T4), 1430 h (T5), 1445 h (T6) and 1500 h (T7) and a thermotolerance index (TCI) was calculated as (10-(T7 to T4)-T1). The statistical analysis was performed by a mathematical model including the fixed effects of breeds and time frames, and the interaction between these effects, besides random effects such as animal and day. The Santa Ines breed presented the lowest RT after sun exposure (39.3 ± 0.12 ◦ C; P < 0.05) and it was the only one to recover morning RT 60 min after heat stress (38.7 and 38.9 for 1300 h and 1500 h; P > 0.05). Hair breeds presented RR lower (P < 0.05) than wool breeds. Although thick wool or hair thickness differs among and within hair and wool breeds (P < 0.05), SR did not differ among breeds and time (227.7 ± 16.44 g m−2 h−1 ; P > 0.05). The thermotolerance index did not differ among breeds, but it showed similar response (P > 0.05) 45 min or 1 h of shade after sun exposure. One week post shearing is not enough to wool breeds present to show thermotolerance similar to hair breeds.