A real time Taqman RT-PCR for the detection of rabbit hemorrhagic disease virus 2 (RHDV2)

As key prey, the wild rabbit downsize constitutes a major drawback on the endangered Iberian lynx (Lynx pardinus) re-introduction in the Iberia. Several captive breeding units mostly located in Alentejo, endeavour the wild rabbit repopulation of depleted areas assigned for the lynx re-introduction. Here we report an RHDV2 outbreak that occurred in early 2016 in a wild rabbit captive breeding unit located in Barrancos municipality. The estimated mortality rate between March and April 2016 was approximately 8.67%. Anatomopathologic examination was carried out for 13 victimized rabbits. Molecular characterization was based on the complete vp60 capsid gene. The 13 rabbit carcasses investigated showed typical macroscopic RHD lesions testing positive to RHDV2- RNA. Comparison of the vp60 nucleotide sequences obtained from two specimens with others publically available disclosed similarities below 98.22% with RHDV2 strains originated in the Iberia and Azores and revealed that the two identical strains from Barrancos-2016 contain six unique single synonymous nucleotide polymorphisms. In the phylogenetic analysis performed, the Barrancos-2016 strains clustered apart from other known strains, meaning they may represent new evolutionary RHDV2 lineages. No clear epidemiological link could be traced for this outbreak where the mortalities were lower compared with previous years. Yet, network analysis suggested a possible connection between the missing intermediates from which the strains from Barrancos 2013, 2014 and 2016 have derived. It is therefore possible that RHDV2 has circulated endemically in the region since 2012, with periodic epizootic occurrences. Still, six years after its emergence in wild rabbits, RHDV2 continues to pose difficulties to the establishment of natural wild rabbit populations that are crucial for the self-sustainability of the local ecosystems.

Zeitgeist or chameleon? A quantitative analysis of CSR definitions.

Despite its increasing relevance, corporate social responsibility (CSR) remains hobbled by problems, variously charged as being chameleon, vacuous or an utterly meaningless concept. One reason is the absence of an agreed upon normative basis underpinning CSR. This is in large part due to the concept lacking a universally accepted definition. This paper explores how the concept of CSR has evolved over time drawing from 110 definitions of the construct. Using co-word analysis of definitions from 1953 to 2014, the study maps how the structure of the definitions has evolved during the field's historical development. The research uncovers the key terms underpinning the phenomenon, the centrality of these terms as well as mapping their interrelationships and evolution. The findings suggest that, despite the profusion and definitional heterogeneity over the six decades of the development of the field, there are six recurrent, enduring dimensions that underpin the CSR concept. These dimensions are economic, social, ethical, stakeholders, sustainability and voluntary. This paper makes several contributions to the academic literature. The systematic, quantitative analysis of definitions brings an objectivity that previous qualitative bibliometric analyses of CSR have lacked. The time period selected is substantially longer than previous analyses and captures the complete historical evolution of the concept. Moreover, the analysis provides the basis for the development of a new, comprehensive, yet concise, definition of CSR that captures all six of the recurring dimensions underpinning the concept.