Lineage, Marriage, and Social Mobility: the Teles de Meneses Family in the Iberian Courts (Fifteenth and Sixteenth Centuries)

This article discusses the social mobility strategies of the Teles de Meneses family throughout the fifteenth and sixteenth centuries, seeking to understand their influence on the family’s social evolution and improved ranking at the court. Marriage policy and service in the Iberian courts are analyzed over three different generations and from two standpoints: first, the preservation of the family’s preacquired status; second, the diversification of the services performed in the various settings where its influence could be exercised. This will highlight the reasons behind the social evolution of this family and the subsequent granting of titles to some of its members.

Fluorescence of nitrobenzoxadiazole (NBD)-labeled lipids in model membranes is connected not to lipid mobility but to probe location

Nitrobenzoxadiazole (NBD)-labeled lipids are popular fluorescent membrane probes. However, the understanding of important aspects of the photophysics of NBD remains incomplete, including the observed shift in the emission spectrum of NBD-lipids to longer wavelengths following excitation at the red edge of the absorption spectrum (red-edge excitation shift or REES). REES of NBD-lipids in membrane environments has been previously interpreted as reflecting restricted mobility of solvent surrounding the fluorophore. However, this requires a large change in the dipole moment (Dm) of NBD upon excitation. Previous calculations of the value of Dm of NBD in the literature have been carried out using outdated semi-empirical methods, leading to conflicting values. Using up-to-date density functional theory methods, we recalculated the value of Dm and verified that it is rather small (B2 D). Fluorescence measurements confirmed that the value of REES is B16 nm for 1,2-dioleoyl-sn-glycero-3- phospho-L-serine-N-(NBD) (NBD-PS) in dioleoylphosphatidylcholine vesicles. However, the observed shift is independent of both the temperature and the presence of cholesterol and is therefore insensitive to the mobility and hydration of the membrane. Moreover, red-edge excitation leads to an increased contribution of the decay component with a shorter lifetime, whereas time-resolved emission spectra of NBD-PS displayed an atypical blue shift following excitation. This excludes restrictions to solvent relaxation as the cause of the measured REES and TRES of NBD, pointing instead to the heterogeneous transverse location of probes as the origin of these effects. The latter hypothesis was confirmed by molecular dynamics simulations, from which the calculated heterogeneity of the hydration and location of NBD correlated with the measured fluorescence lifetimes/REES. Globally, our combination of theoretical and experiment-based techniques has led to a considerably improved understanding of the photophysics of NBD and a reinterpretation of its REES in particular.

Exploring chalcolithic diet and mobility of humans and animals from Perdigões site

Chalcolithic diet at Perdigões (Reguengos de Monsaraz) was investigated using stable carbon and nitrogen isotope analysis of human bone collagen. Strontium isotopes of faunal dental enamel were used to establish the site local range and to distinguish the presence of non-local individuals. ATR-FTIR and EA analysis were used to determine the degree of bone diagenesis and to evaluate the reliability of the stable isotopic composition for paleodietary reconstruction. The individuals from which paleodietary results were obtained had a diet based on C3 terrestrial resources and some animal protein. Data from Perdigões site, was compared with the published data from other Iberian Chalcolithic populations. Site comparison revealed that diet through Iberian Peninsula have mainly maintained terrestrial dietary focus consistent with animal husbandry and farming on C3 plants with occasional intake of freshwater or marine resources. Strontium isotopic composition of enamel revealed that some of the individuals from Perdigões site were non-local; Resumo: A dieta de indivíduos do período Calcolítico dos Perdigões (Reguengos de Monsaraz) foi investigada através de análise de isótopos estáveis de carbono e azoto no colagénio ósseo. Isótopos de estrôncio do esmalte dentário de fauna foram utilizados para estabelecer o sinal local dos Perdigões, permitindo distinguir a presença de alguns indivíduos e fauna não-locais. A avaliação da diagénese óssea foi efectuada através de análises realizadas com analisador elementar e com ATR-FTIR, de forma a validar dos resultados isotópicos obtidos. Os indivíduos para os quais foi possivel obter resultados isotópicos de carbono e azoto apresentam uma dieta baseada em recursos terrestres, plantas C3, e alguma proteína animal. A comparação efectuada com outros sitios arqueológicos da Peninsula Ibérica revelaram que a dieta das populações é sobretudo feita à base de plantas C3, com ingestão de alguma proteina animal e ingestão ocasional de produtos marinhos ou provenientes de água doce.