Fixing, storage time and DNA extraction applied to dT-RFLP for ecological and molecular studies of marine nematodes assemblages

The application of molecular methods offers an alternative faster than traditional methods based on morphology It is nearly impossible to process all the samples in short period using traditional methods, and the deterioration of marine sediments rapidly occurs The dT-RFLP (directed Terminal-Restriction Fragment Length Polymorphism) allows a rapid assessment of biodiversity changes of nematodes assemblages The use of a not suitable fixing, storage time and DNA extraction could be a limitation in molecular analysis like dT-RFLP and real time PCR.Objetives: the best fixative •the level of DNA degradation over the time •the best DNA extraction method for marine nematodes and suitable for dT-RFLP analysis

Acoustic Propagation over Periodic and Quasi-Period Rough Surfaces Proceedings

Numerical techniques such as the Boundary Element Method, Finite Element Method and Finite Difference Time Domain have been used widely to investigate plane and curved wave-front scattering by rough surfaces. For certain shapes of roughness elements (cylinders, semi-cylinders and ellipsoids) there are semi-analytical alternatives. Here, we present a theory for multiple scattering by cylinders on a hard surface to investigate effects due to different roughness shape, the effects of vacancies and variation of roughness element size on the excess attenuation due to a periodically rough surfaces.

Calibration of Grassmaster II to estimate green and dry matter yield in Mediterranean pastures: effects of pasture moisture content

Accurate assessment of standing pasture biomass in livestock production systems is a major factor for improving feed planning. Several tools are available to achieve this, including the GrassMaster II capacitance meter. This tool relies on an electrical signal, which is modified by the surrounding pasture. There is limited knowledge on how this capacitance meter performs in Mediterranean pastures. Therefore, we evaluated the GrassMaster II under Mediterranean conditions to determine (i) the effect of pasture moisture content (PMC) on the meter’s ability to estimate pasture green matter (GM) and dry matter (DM) yields, and (ii) the spatial variability and temporal stability of corrected meter readings (CMR) and DM in a bio-diverse pasture. Field tests were carried out with typical pastures of the southern region of Portugal (grasses, legumes, mixture and volunteer annual species) and at different phenological stages (and different PMC). There were significant positive linear relations between CMR and GM (r2 = 0.60, P < 0.01) and CMR and DM (r2 = 0.35, P < 0.05) for all locations (n = 347). Weak relationships were found for PMC (%) v. slope and coefficient of determination for both GM and DM. A significant linear relation existed for CMR v. GM and DM for PMC >80% (r2= 0.57, P < 0.01, RMSE = 2856.7 kg ha–1, CVRMSE=17.1% to GM; and r2= 0.51, P < 0.01,RMSE = 353.7 kg ha–1, CVRMSE = 14.3% to DM). Therefore, under the conditions of this current study there exists an optimum PMC (%) for estimating both GM and DM with the GrassMaster II. Repeated-measurements taken at the same location on different dates and conditions in a bio-diverse pasture showed similar and stable patterns between CMR and DM (r2= 0.67, P < 0.01, RMSE = 136.1 kg ha–1, CVRMSE = 6.5%). The results indicate that the GrassMaster II in-situ technique could play a crucial role in assessing pasture mass to improve feed planning under Mediterranean conditions.

The Palace of Quinta do Marquês do Alegrete: restitution strategies for space, time and memory

Palaces, as an architectural typology, can be found in recreation Quintas that surrounded the main cities in Portugal, which preserved its rural character since the 16th century until the middle of the 19th century. Consisting of cultivated land and farm buildings, the palace of the Quinta was the owner´s temporary residence, for summer holidays and festive events, with gardens, pavilions, fountains and lakes for recreational purposes and leisure. The focus on palaces, as a historic building and as in need of new uses, clearly shows how current the debate on contemporary interventions in this heritage typology is. Interventions in architectural heritage require multidisciplinary teams to identify conservation strategies which enable a qualified use of its spaces, such as for example the experience of security and well-being, which can contribute to a better quality of life and simultaneously to the quality of the urban environment. This paper presents the Palace of Quinta Alegre and its rehabilitation project for contemporary use and public esteem, both of which are considered fundamental prerequisites for its sustainable maintenance in space, in time and in memory. [versão Portuguesa] Sob a denominação de tipologia arquitectónica, o edifício Palácio pode ser encontrado nas Quintas de Recreio que rodeavam as principais cidades Portuguesas, tendo preservado o seu carácter rural, desde o século XVI até metade do século XIX. Consistindo as Quintas em terra cultivada e edifícios rurais, o Palácio da Quinta consistia na residência temporária do proprietário, para férias de verão e eventos comemorativos, dispondo de jardins, pavilhões, fontes e lagos para recreação e lazer. O tema dos Palácios, entendido como edifício histórico que procura novos usos, demonstra como é actual o debate sobre intervenções contemporâneas nesta tipologia de valor patrimonial. A intervenção em património arquitectónico requer a definição de estratégias de conservação por equipas multidisciplinares que permitam estabelecer um uso qualificado dos seus espaços, proporcionando experiências sensoriais de bem-estar e segurança, contribuindo para uma melhor qualidade de vida e, simultaneamente, para a qualidade do ambiente urbano em que se insere. Este artigo tem por objectivo apresentar o Palácio da Quinta Alegre e o projecto de reabilitação, devolvendo-o a um uso contemporâneo e à estima pública, factores fundamentais para a sua manutenção sustentável no espaço, no tempo e na memória.